ISOLATION OF COMMON CARP b-ACTIN PROMOTER

Abstrak

Promoter in the transgene construct plays an important
role on regulating of transgene expression level
in transgenic fish. In fish transgenesis, researcher
convinced that use all-fish promoter more safety
and prospective. This study was performed to isolate
â-actin promoter, - the promoter which has ubiquitous,
constitutive, house keeping characteristics, from
common carp. -actin promoter from common carp
(ccBA) was isolated using PCR method with FBP1,
RBP1 and RBP2 primers. Sequencing was performed
using ABI PRISM 3100 machine and analysis of
sequences was conducted using GENETYX version 7
software. The results of sequence analysis showed that
the length of DNA fragment obtained approximately
1,5 kb and containing the evolutionary conserved
sequences of transcription factor for â-actin promoter
including CCAT, CArG and TATA box. The results
demonstrated that isolated sequence was a common
carp â-actin promoter.
Key words: Homologous promoter, GFP, carp transgenic