Study of Streptomyces spp. to control purple blotch disease caused by Alternaria porri in shallot plant

Rateh Lailatul Risdiyanti, Noni Rahmadhini, Penta Suryaminarsih, Tri Mujoko

Abstract


Alternaria porri is a fungal pathogen that causes purple blotch on shallots, this fungus can cause crop yield loss of 3 – 57%. A. porri was obtained from the Sumber Brantas onion farm and then isolated by planting infected tissue. Streptomyces spp. is a filamentous bacterium that is abundant in the soil and can be used as a biological agent, decomposer and plant growth promote. Streptomyces spp. was obtained one isolate from shallot land location in Pare, Kediri, East Java (BMP: Bawang Merah Pare) and three isolates from Sidera, Palu, Central Sulawesi (BMS: Bawang Merah Sidera) (BMS1, BMS2, BMS3). The purpose of this study was to determine the efficacy of Streptomyces spp. to control A porri consist of in vitro and In vivo antagonist test. Antagonist test was carried out by dual culture method and the Streptomyces spp. which can inhibit the development of A porri would be used antagonist test   in vivo. The study was designed using a Factorial Complete Randomized Design with two factors, candidate isolates of biological control agents and concentrations of Streptomyces spp. be diluted into 5%, 10%, and 15%, it will applied on shallot plant Bauji variety. The results of the In vitro test shown the highest inhibition zone from BMP 17.75% while BMS1 13.75%, BMS2 8,75%, and 8.50%. in vivo test shown lowest disease severity value in BMP 15% concentration was 1.13% while diseases severity of BMS1 was 1.27%, and BMS2 was 1.80%. Therefore, Streptomyces spp. has potency as an alternative pesticide for sustainable agriculture.

Keywords


Biological control, Antagonist, diseases severity

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References


Anjarsari, DT, Prasetyawati ET, & Wuryandari Y. (2021). Inhibitory Test of Bacillus sp. against Phytophthora palmivora Causes Cocoa Fruit Rot Disease. Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur, 2022. https://doi.org/https://doi.org/ 10.11594/nstp.2022.2003

Fardiyanti R, Kasrina K, & Bustamam H. (2021). Various types of Streptomyces sp in the rizosphere of the liliacea tribe in the village of sumber bening. Konservasi Hayati, 17(1), 29–34. https://doi.org/https://doi.org/10.33369/ hayati.v17i1.14731

Galande S, Chitale R, Wangikar H, Wagh S, D. A. and B. P. (2020). Isolation and Identification of Aeromycoflora in Banana Field from Baramati Area Dist. Pune, Maharashtra, India.

Handayani NMDW, Muthahanas I, & Nikmatullah A. (2021). Application of biopesticides Streptomyces sp. in controlling disease in potato (Solanum tuberosum L.) plants in the medium plain. agroteksos, 30(2), 109–124. https://doi.org/https://doi.org/10.29303/agroteksos.v30i2.701

Hersanti H, Sudarjat S, & Damayanti A. (2019). The ability of Bacillus subtilis and Lysinibacillus sp. in silica nano and carbon fiber to induce shallot resistance to purple spotted disease (Alternaria porri (Ell.) Cif). Agrikultura, 30(1), 8–16. https://doi.org/https://doi.org/10. 24198/agrikultura.v30i1.22698

Mohsin SM, Islam MR, Ahmmed ANF, Nisha HAC, & Hasanuzzaman M. (2016). Cultural, morphological and pathogenic characterization of alternaria porri causing purple blotch of onion. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44(1), 222–227. https://doi.org/10.15835/nbha44110110

Nurosid IS, Nurdiana D, & Tauhid A. (2018). Effect of various concentrations of biological agent solutions on purple spot disease (Alternaria porri), growth and yield of shallot (Allium ascalonicum L.) variety tuk-tuk. Jagros: Journal of agrotechnology science, 3(1), 39–50. https://doi.org/http://dx.doi.org/10.52434 /jagros.v3i1.452

Purnomo E, Mukarlina R, & others. (2017). Streptomyces spp. bacterial antagonist test against Phytophthora palmivora BBK01 Fungus Causes of Fruit Rot on Cocoa Plants. Journal Protobiont, 6(2). https://doi.org/:http: //dx.doi.org/10.26418/protobiont.v6i2.20795

Puspita ES, & Yulianti L. (2016). Fuzzy logic based weather forecasting system design. Journal Media Infotama, 12(1). https://doi.org/https:// doi.org/10.37676/jmi.v12i1.267

Putri RA. (2018). Effectiveness of rhizobacteria Streptomyces sp. to suppress pepper yellow leaf curl virus on chili in the field. Journal Fitopatologi Indonesia, 14(5), 183. https://doi.org/https://doi.org/10.14692/jfi.14.5.183

Queendy V, & Roza RM. (2019). Antifungal activity of actinomycetes from arboretum university of riau against Fusarium oxysporum f.sp lycopersici and ganoderma boninense. Journal of Biology 12(1), 73–88. https://doi.org/https://doi.org/10.15408/kauniyah.v12i1.8793

Raharini AO, Kawuri R, & Khalimi K. (2012). The use of Streptomyces sp. as biocontrol of wilt disease in red chili (Capsicum annuum L.) caused by Fusarium oxysporum f. sp. capsici. Journal of Agrotrop, 2(2), 151–159.

Putri RJ, Kawuri R, Darmadi AAK, & Narayani I. (2021). Potential of Streptomyces sp. in preventing the in vitro growth of Colletotrichum acutatum, the causative agent of infection in Capsicum annum L. Journal of Udayana Biology. 25(2), 197–207, https://doi.org/10.24843/JBIOUNUD.2021.v25.i02.p12

Setyawati TR, Kurniatuhadi R, & Yanti AH. (2021). Colony Morphological Characters of Streptomyces Spp. which was isolated from Nipah Worm Habitat Substrate (Namalycastis Rhodochorde) on Different Medium.. Prosiding Seminar Nasional Penerapan Ilmu Pengetahuan Dan Teknologi, 147–154.

Suryaminarsih P, Harijani, WS, Mindari W, & Wurjani W. (2018). Study of humic acid and multiantagonis of Streptomyces sp, Trichoderma sp application techniques for horticulture plant on marginal soil. 1(Icst), 249–252. https://doi.org/10.2991/icst-18.2018.52

Suryaminarsih P, Harijani WS, Syafriani E, Rahmadhini N, & Hidayat R. (2019). Application of Streptomyces sp. as a biological agent for controlling fruit flies (Bactrocera sp.) and plant growth promoting bacteria (PGPB) in tomato and chili plants. AGRIUM: Journal of Agricultural Sciences, 22(1), 62–69. http://dx.doi.org/10.30596%2 Fagrium.v22i1.3106

Suryaminarsih P, Megasari D, & Mujoko T. (2020). the effect of temperature pressure on multiantagonists Streptomyces sp., Tricho-derma sp. biological control of Fusarium oxysporum Wilt Pathogens . Biological Control of . 2020, 85–91. https://doi.org/https://doi.org/10.11594/nstp.2020.0609

Suryaminarsih P, & Mujoko T. (2020). Competition of biological agents of Streptomyces sp, Gliocladium sp, and Trichoderma harzianum to Fusarium oxysporum in tomato rhizophere. CROPSAVER-Journal of Plant Protection, 3(1), 17–21. https://doi.org/10.24198/ cropsaver.v3i1.24173

Syahrok SF, Widiyati, W, Pribadi DU, Wiyatiningsih, S., & Suryaminarsih, P. (2021). Effect of giving Trichoderma sp. and Streptomyces sp. on the existence of root pull nematode and the growth of cherry tomato plants. Journal of AGROHITA: Journal of Agrotechnology, Faculty of Agriculture, Muhammadiyah University of South Tapanuli, 6(2), 132–138. https://doi.org/http://dx.doi.org/10.31604/jap.v6i2.4109

Vurukonda SSKP, Giovanardi D, & Stefani E. (2018). Plant growth promoting and biocontrol activity of Streptomyces spp. as endophytes. International Journal of Molecular Sciences, 19(4), 952. https://doi.org/https://doi.org/10. 3390/ijms19040952




DOI: https://doi.org/10.24198/cropsaver.v6i1.43647

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