PERKEMBANGAN POLYMERASE CHAIN REACTION (PCR) DAN RAPID DIAGNOSTIC TEST (RDT) SEBAGAI PENGUJIAN COVID-19

KENNY DWI SIDHARTA, JAMES PRASETYO LAKSONO, TINA ROSTINAWATI

Abstrak


Pandemi COVID-19 yang disebabkan oleh SARS-CoV-2 saat ini tengah menjadi masalah kesehatan global. Bila tidak ditangani dengan baik, penyakit ini dapat menyebabkan komplikasi fatal terutama pada pasien-pasien tertentu. Salah satu tahapan dalam menegakkan diagnosis COVID-19 adalah dengan uji laboratorium. Saat ini, WHO telah menetapkan beberapa metode untuk pengujian laboratorium COVID-19, di antaranya menggunakan PCR dan RDT. Artikel ini mengulas perkembangan metode PCR dan RDT saat ini sebagai pengujian COVID-19.  Ditemukan bahwa sampai saat ini metode PCR lebih direkomendasikan dalam pemeriksaan infeksi SARS-CoV-2 sementara pengembangan dan validasi dari metode RDT masih diperlukan untuk meningkatkan efektifitasnya.


Teks Lengkap:

PDF

Referensi


Bachman, J., 2013. Reverse-Transcription PCR (RT-PCR). Methods in Enzymology, Volume 530, pp. 67-74.

Bai, Y. et al., 2020. Presumed Asymptomatic Carrier Transmission of COVID-19. JAMA, 323(14), pp. 1406-1407.

Biswas, A. et al., 2020. Emergence of Novel Coronavirus and COVID-19: whether to stay or die out?. Critical Reviews in Microbiology. DOI: 10.1080/1040841X.2020.1739001.

Bruning, A. H. L. et al., 2017. Rapid Tests for Influenza, Respiratory Syncytial Virus, and Other Respiratory Viruses: A Systematic Review and Meta-analysis. Clinical Infectious Diseases, 65(6), pp. 1026-1032.

Chan, J.-W.et al., 2020. Improved molecular diagnosis of COVID-19 by the novel, highly sensitive and specific COVID-19-RdRp/Hel real-time reverse transcription-polymerase chain reaction assay validated in vitro and with clinical specimens. J. Clin. Microbiol. DOI: 10.1128/JCM.00313-20.

Chen, N. et al., 2020. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. Lancet, 395(10233), pp. 507-513.

Chu, D. K. W. et al., 2020. Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia. Clinical Chemistry, 66(4), pp. 549-555.

Corman, V. M. et al., 2020. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill, 25(3), p. pii=2000045.

Giesker, K. & Hensel, M., 2014. Bacterial Vaccines. Germany: Elsevier.

Goldenberg, S., 2013. Molecular-based diagnostics, including future trends. Medicine (Abingdon), 31(11), pp. 663-666.

Grody, W. W., Nakamura, R. M., Strom, C. M. & Kiechle, F. L., 2010. Molecular Diagnostics: Techniques and Applications for the Clinical Laboratory. Academic Press.

Li, Q. et al., 2020. Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus–Infected Pneumonia. N Engl J Med, Volume 382, pp. 1199-1207.

Lu, H., Stratton, C. W. & Tang, Y.-W., 2020. Outbreak of pneumonia of unknown etiology in Wuhan, China: The mystery and the miracle. J Med Virol, 92(4), pp. 401-402.

Maddocks, S. & Jenkins, R., 2016. Understanding PCR: A Practical Bench-Top Guide. Academic Press.

Marx, V., 2015. PCR heads into the field. Nat Methods, Volume 12, pp. 393-397.

Nao, N. et al., 2020. Detection of second case of 2019-nCoV infection in Japan. [Online]

Available at: https://www.niid.go.jp/niid/en/2019-ncov-e/9334-ncov-vir3-2.html

[Accessed 1 October 2020].

Okba, N. M. A. et al., 2020. SARS-CoV-2 specific antibody responses in COVID-19 patients. Emerging Infectious Disease. DOI: https://doi.org/10.1101/2020.03.18.20038059.

Petherick, A., 2020. Developing antibody tests for SARS-CoV-2. The Lancet, 395(10230), pp. 1101-1102.

Sheridan, C., 2020. Fast, portable tests come online to curb coronavirus pandemic. Nature Biotechnology, Volume 38, pp. 509-522.

Sohrabi, C. et al., 2020. World Health Organization declares global emergency: A review of the 2019 novel coronavirus (COVID-19). Int J Surg, Volume 76, pp. 71-76.

Wang, D. et al., 2020. Clinical Characteristics of 138 Hospitalized Patients With 2019 Novel Coronavirus–Infected Pneumonia in Wuhan, China. JAMA, 323(11), pp. 1061-1069.

World Health Organization, 2020. Advice on the use of point-of-care immunodiagnostic tests for COVID-19. [Online]

Available at: https://www.who.int/news-room/commentaries/detail/advice-on-the-use-of-point-of-care-immunodiagnostic-tests-for-covid-19

[Accessed 1 October 2020].

World Health Organization, 2020. WHO Virtual press conference on COVID-19. [Online]

Available at: https://www.who.int/docs/default-source/coronaviruse/transcripts/who-audio-emergencies-coronavirus-press-conference-full-and-final-11mar2020.pdf?sfvrsn=cb432bb3_2

[Accessed 1 October 2020].

World Health Organization, 2020. Laboratory testing for coronavirus disease 2019 (‎‎COVID-19)‎‎ in suspected human cases: interim guidance. [Online]

Available at: https://apps.who.int/iris/handle/10665/331501

[Accessed 1 October 2020].

Ye, F. et al., 2020. Delivery of Infection from Asymptomatic Carriers of COVID-19 in a Familial Cluster. International Journal of Infectious Diseases. DOI: https://doi.org/10.1016/j.ijid.2020.03.042.

Yip, C.-Y.et al., 2020. Development of a Novel, Genome Subtraction-Derived, SARS-CoV-2-Specific COVID-19-nsp2 Real-Time RT-PCR Assay and Its Evaluation Using Clinical Specimens. Int. J. Mol. Sci., 21(7), p. 2574.

Zhao, J. et al., 2020. Antibody Responses to SARS-CoV-2 in Patients of Novel Coronavirus Disease 2019. Clinical Infectious Disease. DOI: 10.1093/cid/ciaa344.




DOI: https://doi.org/10.24198/farmaka.v19i1.29823

DOI (PDF): https://doi.org/10.24198/farmaka.v19i1.29823.g16020

Refbacks

  • Saat ini tidak ada refbacks.




Sitasi manajer:   

 

 

Jurnal ini diindeks dalam:

 

 

View My Stats 

ISSN: 1693-1424

e-ISSN: 2716-3075

 

Farmaka by Universitas Padjadjaran is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

Copyright © 2013 Jurnal Farmaka - All Right Reserved