AKTIVITAS DEPIGMENTASI FRAKSI N-HEKSANA BUAH MALAKA (PHYLLANTHUS EMBLICA) PADA SEL MELANOSIT MOUSE MELANOMA B16

Abstract

Abstract

Inhibitor of melanin synthesis can be used as depigmenting agent to treat skin hyperpigmentation. Herbal depigmenting agents are increasingly utilized due to their character in inhibiting melanin synthesis without causing melanocyte toxicity. This study was aimed to examine depigmenting activity of n-hexane fraction of Phyllanthus emblica fruit by measuring its melanin synthesis
inhibition on melanocyte mouse melanoma B16 cell line. Melanin synthesis inhibition was measured spectrophotometrically by counting the melanin and the activity of tyrosinase. The cytotoxicity of n-hexane fraction on melanocytes was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. This study was conducted on November-December
2009 at Department of Biochemistry and Diabetes Research Centre, Chonbuk National University Medical School, South Korea. The result of this study indicated that melanin count and tyrosinase activity decreased in a dose-dependent manner of n-hexane fraction of P. emblica fruit (IC50 31.68 and 77.92 μg/ml, respectively). The viable cells decreased with increasing concentration of
the n-hexane fraction of P. emblica fruit (LD50 87.39 μg/ml).

Key words: n-hexane fraction, Phyllanthus emblica, depigmenting agent