Aktivitas Antibakteri Produk Polen Lebah terhadap Salmonella typhi NCTC786 BCC712

Tiana Milanda, Astri K. Sjafril, Sulistiyaningsih Sulistiyaningsih

Abstrak


Polen lebahmerupakan serbuk sari bunga atau bagian tumbuhan lainnya yang  dikumpulkan oleh lebah madu pejantan dari berbagai tumbuhan. Produk ini diketahui memiliki berbagai aktivitas farmakologi, diantaranya sebagai antimikroba terhadap beberapa bakteri Gram positif, Gram negatif dan jamur. Sampai saat ini, belum ada informasi mengenai aktivitas antibakteri polen lebah terhadap Salmonella typhi, penyebab demam tifoid pada manusia. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri beberapa produk polen lebah dari Kota Bandung, Jawa Barat, Indonesia terhadap S. typhi NCTC786 BCC712. Penelitian ini dilakukan melalui tahap pengujian aktivitas antibakteri produk polen lebah, penentuan nilai Konsentrasi Hambat Tumbuh Minimum (KHTM) dan Konsentrasi Bunuh Minimum (KBM) produk polen lebah teraktif dan penentuan nilai banding aktivitas antibakterinya  terhadap kloramfenikol.Hasil penelitian menunjukkan ketiga produk mempunyai aktivitas antibakteri terhadap S. typhi NCTC786 BCC712, dengan aktivitas terbesar ditunjukkan oleh produk ketiga. KHTMdan KBM dari produk polen lebah terhadap S. typhi NCTC786 BCC712 masing-masing sebesar 137,5 mg/mL dan140 mg/mL.Nilai banding aktivitas antibakteri produk tersebut terhadap  dengan kloramfenikol sebesar 1 : 0,0562. Hal ini menunjukkan aktivitas antibakteri produk polen lebah ketiga terhadap S. typhi NCTC786 BCC712 lebih lemah dibanding kloramfenikol.

Teks Lengkap:

PDF

Referensi


Babinska I, Kleczek K, Szarek J, Makowski W. Modulating effect of propolis and bee pollen on chicken breeding parameters and pathomorphology of liver and kidneys in the course of natural infection with Salmonella enteritidis. Bull Vet Inst Pulawy. 2012;56:3-8.

Mărgăoan R, Mărghitaş LA, Dezmirean DS, Bobiș O, Mihai CM, Physical-chemical composition of fresh bee pollen from Transylvania. Bulletin UASVM Animal Sci Biotechnol. 2012;69(1-2): 351-355.

Bogdanov S. Pollen: nutrition, functional properties, health: A review [diunduh 2014]. Tersedia dari http://www.bee-hexagon.net.

Nogueira C, Iglesias A, Xesus F, Estevinho LAM, Commercial bee pollen with different geographical origins: A comprehensive approach, International J Mol Sci. 2012;13(9);11173- 11187.

Chantarudee A, Phuwapraisirisan P, Kimura K, Okuyama M, Mori H, Kimura A, et al. Chemical constituents and free radical scavenging activity of corn pollen collected from Apis mellifera hives compared to floral corn pollen at Nan, Thailand, BMC Complementary and Alternative Medicine. 2012;12(45):1472, 6882.

Mohdaly AAA, Mahmoud AA, Roby MHH, Smetanska I, Ramadan MF. Phenolic extract from propolis and bee pollen composition, antioxidant and antibacterial activities, J Food Biochem. 2015;39:538-547.

Morais M, Moireira L, Feas X, Estevinho LM. Honeybee-collected pollen from five Portuguese Natural Parks: Palynological origin, phenolic content, antioxidant properties and antimicrobial activity. Food Chem Toxicol. 2011;49(5):110, 1096.

Fatrcova-Sramkova K, Nozkova J, Kacaniova M, Mariassyova M, Rovna K, Stricik M. Antioxidant and antimicrobial properties of monofloral bee pollen. J Environ Sci Health. Part B. Pesticides, Food Contaminants and Agricultural Wastes. 2013;48(2):133-138.

Spulber R, Dogaroglu M, Babeanu N, Popa O. Physicochemical characteristics of fresh bee pollen from different botanical origins. Romanian Biotechnol Letters. 2018;23(1):13357-13365.

Pascoal A, Rodrigues S, Teixeira A, Feas X, Estevinho LM, Biological activities of commercial bee pollens: Antimicrobial, antimutagenic, antioxidant and anti-inflammatory, Food Chem Toxicol. 2014;63;233-239.

Glusac JR, Stijepic MJ, Milanovic SD, Milosevic DMD. Physicochemical properties of honeybee pollen enriched acidophilus milk and probiotic yoghurt, Biblid. 2015;46:45-54.

Sun L, Guo Y, Zhang Y, Zhuang Y, Antioxidant and anti-tyrosinase activities of phenolic extracts from rape bee pollen and inhibitory melanogenesis by cAMP/MITF/TYR pathway in B16 mouse melanoma cells. Front Pharmacol. 2017;8:104.

Salles J, Cardinault N, Patrac V, Berry A, Giraudet C, Collin ML, et al. Bee pollen improves muscle protein and energy metabolism in malnourished old rats through interfering with the mtor signaling pathway and mitochondrial activity. Nutrients. 2014;6(12):5500-5516.

Rinayanti A, Rahayu ST. The antibacterial effects of essential oil from galangal rhizome Alpinia galanga (Linn.) pierreon rat (Rattus novergicus L.) were infected by Salmonella typhi. Asian J Pharm Clin Res. 2016;9(Suppl 1):189-193.

Milanda T, Barliana MI, Rosidah, Kusuma ASW. Antibacterial activities of Parijoto (Medinilla speciosa) against clinical isolates of Salmonella typhi and Shigella dysentriae. Pharmacology and Clinical Pharmacy Research. 2021;6(1): 25-34.

Khider M, Elbanna K, Mahmoud A, Owayss AA. Egyptian honeybee pollen as antimicrobial, antioxidant agents, and dietary food supplements. Food Sci Biotechnol. 2013;22:1461–1469.

Kai Y, Dan W, Xingqian Y, Donghong L, Jianchu C, Peilong S. Characterization of chemical composition of bee pollen in China. J Agric Food Chem. 2013;61(3):708-718.

Xesus F, Pilar MVT, Estevinho L, Seijas JA, Iglesias A. Organic bee pollen: Botanical origin, nutritional value, bioactive compounds, antioxidant activity and microbiological quality. Molecules. 2012;17(7):8359-8377.

Morgano MA, Martins MCT, Rabonato LC, Milani RF, Yotsuyanagia K. Amaya DBR. A comprehensive investigation of the mineral composition of Brazilian bee pollen: Geographic and seasonal variations and contribution to human diet. J Braz Chem Soc. 2012;23(4):727-736.

Araujo SJ, Chambo ED, Costa MA, Da Silva SMPC, De Carvalho CAL, Estevinho LM. Chemical composition and biological activities of mono- and heterofloral bee pollen of different geographical origins. Int J Mol Sci. 2017;18(5):921.

Solgajova M, Nozkova J, Kadakova M. Quality of durable cookies enriched with rape bee pollen. J Central Euro Agri. 2014;15(1):24-38.




DOI: https://doi.org/10.24198/ijbp.v1i1.35162

DOI (PDF): https://doi.org/10.24198/ijbp.v1i1.35162.g16144

Refbacks

  • Saat ini tidak ada refbacks.




IJBP by Universitas Padjadjaran is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License