Studi In Silico Senyawa Biji Klabet (Trigonella Foenum-Graecum L.) Sebagai Inhibitor Estrogen Alfa Pada Kanker Payudara

Gracia Anastasya, Lena Wensa, Shinta Permata, Asyifa Amatulloh, Zulfa Tavira, Ade Zuhrotun

Abstrak


Abstrak

Prevalensi kanker payudara masih tinggi. Pada tahun 2021 sendiri, kanker payudara menempati urutan pertama sebagai kanker yang umum terjadi pada wanita.  Reseptor dari sel payudara normal dan kebanyakan sel kanker payudara menempel di sirkulasi progesteron dan estrogen. Reseptor estrogen-ɑ merupakan salah satu biomarker sel kanker payudara. Inhibisi ER-ɑ menjadi salah satu target terapi kanker payudara. Senyawa aktif pada biji klabet (Trigonella foenum-graecum L.) telah diketahui memiliki aktivitas antikanker. Dilakukan pengujian prediksi Lipinski Rules of Five, Prediksi Pre-ADMET, dan Penambatan Molekul pada reseptor estrogen-ɑ terhadap 10 senyawa yang terkandung pada biji klabet. Hasil menunjukkan bahwa Diosgenin memiliki potensi sebagai kandidat obat anti kanker payudara dengan nilai energi ikatan -8,34 kkal/mol dan nilai KI 0,77067 uM, namun memiliki nilai %PPB yang tinggi sehingga perlu dilakukan pertimbangan lebih lanjut.

Kata kunci: Biji klabet, estrogen-ɑ, kanker payudara, penambatan molekular


Teks Lengkap:

PDF

Referensi


Depkes RI. Pedoman Penemuan dan Penatalaksanaan Penyakit Kanker Tertentu di Komunitas. Jakarta: Departemen Kesehatan RI; 2009.

World Health Organization. Breast Cancer. [diunduh 01 Maret 2023]. Tersedia dari: https://www.who.int/news-room/fact-sheets/detail/breast-cancer#:~:text=In%202020%2C%20there%20were%202.3,the%20world's%20most%20prevalent%20cancer.

Kapara, A., Brunton, V. G., Graham, D., dan Faulds, K. Characterisation of Estrogen Receptor Alpha (ERα) Expression in Breast Cancer Cells and Effect of Drug Treatment Using Targeted Nanoparticles and SERS. Analyst. 2020; 145(22), 7225-7233. DOI: 10.1039/D0AN01532F.

Yamamoto-Ibusuki, M., Arnedos, M., dan Andre, F. Targeted Therapies for ER+/HER2 Metastatic Breast Cancer. BMC Medicine. 2015; 13. 137

Plant, T.M. dan Zeleznik, A.J. (2015). Knobil and Neill's Physiology of Reproduction Fourth Edition. USA: Elsevier.

Kaviarasan, M. Ramamurty, N., Gunasekaran, P., Varalakshmi, E., Anuradha, C. V. Fenugreek (Trigonella Foenum Graecum) Seed Extract Prevents Ethanol-Induced Toxicity And Apoptosis In Chang Liver Cells. Alcohol & Alcoholism. 2006; 41(3) 267–273.

Chaudhary, S., Chaudhary, P.S., Chikara, S.K., Sharma, M.C. and Iriti, M. Review on Fenugreek (Trigonella foenum-graecum L.) and Its Important Secondary Metabolite Diosgenin. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2018; 46(1): 22-31.

Agustini, K., Wiryowidagdo, S. and Kusmana, D. Cytotoxic Activity on MCF-7 Cells and in Silico Study of Sapogenin Steroids from Trigonella foenum-graecum L. Indonesian Journal of Cancer Chemoprevention. 2015; 6(1):1-6.

Nagore, D., Patil, P., and Kuber, V. Comparison between high performance liquid chromatography and high performance thin layer chromatography determination of Diosgenin from fenugreek seeds. International Journal of Green Pharmacy. 2012; 6(4): 315.

El Bairi. Holistic approach of Trigonella foenum-graecum in Phytochemistry and Pharmacology- A Review. Current Trends in Technology and Science. 2017; 3(1): 34-48.

Singh, P., Bajpai, V., Gond, V., Kumar, A., Tadigoppula, N., and Kumar, B. Determination of bioactive compounds of fenugreek (Trigonella foenum-graecum) seeds using LC-MS techniques. In Legume Genomics. Humana, New York, NY; 2020.

He, Y., Wang, X., Suo, Y., Ding, C., and Wang, H. Efficient protocol for isolation of rhaponticin and rhapontigenin with consecutive sample injection from fenugreek (Trigonella foenum-graecum L.) by HSCCC. Journal of chromatographic science. 2016; 54(3): 479-485.

Michael, D., and D. Kumawa. Legend and archeology of fenugreek, constitutions and modern applications of fenugreek seeds. International Sympozium, USA. 2003; 41-42.

Makiyah, A., dan Sumirat T. Uji Toksisitas Akut yang Diukur dengan Penentuan LD50 Ekstrak Etanol Umbi Iles-iles (Amorphophallus variabilis Bl.) pada Tikus Putih Strain Wistar. MKB. 2017; 49(3):145- 155.

Lipinski, C. A. Drug-like properties and the causes of poor solubility and poor permeability. J. Pharmacol. Toxicol. Methods. 2000; 44 (1): 235–249.

Shay, A. C., Powers, J., Doescher, T. A. Adult-Gerentology Clinical Nurse Specialist Certification Review. Newyork: Springer Publishing; 2021.

Syahputra, G., Ambarsari, L., dan Sumaryada, T. Simulasi Docking Kurkumin Enol, Bisdemetoksi Kurkumin dan Analognya sebagai Inhibitor Enzim 12-Lipoksigenase. Jurnal Biofisika. 2011; 10(1), 55-67.

Prasetiawati, R., Suherman, M., Permana, B., & Rahmawati, R. Molecular docking study of anthocyanin compounds against Epidermal Growth Factor Receptor (EGFR) as anti-lung cancer. Indonesian Journal of Pharmaceutical Science and Technology, 2021; 8(1), 8-20.

Cheng, J., Palva, A.M., de Vos, W.M., dan Satokari, R. Contribution of the intestinal microbiota to human health: from birth to 100 years of age. Curr Top Microbiol Immunol. 2013; 358: 323-46.

Prasetiawati R, Suherman M, Permana B, Rahmawati. Molecular Docking Study of Anthocyanidin Compounds Against Epidermal Growth Factor Receptor (EGFR) as Anti-Lung Cancer. IJPST. 2021; 8(1): 8-20

Kumar, R., Giri, A., Nadendla, R.R. In Silico ADME Profiling of CDK9 Inhibitors. Journal of Scientific Research in Pharmacy. 2018; 7(3): 30-4.

Suherman M, Prasetiawati R, Ramdani D. Virtual Screening Of Tamarind Active Compounds (Tamarindus Indica L.) On Selective Inhibitor Siklooksigenase-2. Jurnal Ilmiah Farmako Bahari. 2020;11(2): 135

Hardjono, S. Prediksi sifat farmakokinetik, toksisitas dan aktivitas sitotoksik turunan N-benzoil-N’-(4-fluorofenil) tiourea sebagai calon obat antikanker melalui pemodelan molekul. Jurnal Ilmu Kefarmasian Indonesia. 2017; 14(2), 246-255. ISSN 2614-6495.

Hsu KH, Su BH, Tu YS, Lin OA, and Tseng YJ. Mutagenicity in a Molecule:Identification of Core Structural Features of Mutagenicity Using a Scaffold Analysis. PLoS One. 2016; 11(2).

Noviardi HME, Ramadhan R. Penapisan Molekular Kandidat Obat Sintetik Tuberkulosis terhadap Protein Tirosin Kinase Mycobacterium tuberculosis. Jurnal Farmamedika. 2020; 5(2): 60-69

Ferreira, L DosDantos, R., Olivia, G., Andricopulo, A. Molecular Docking and Structure-based Drug Design Strategies. Molecules. 2015; 20 (2): 384-421.

Chang Y, Min J, Jarusiewicz J.A., Actis M, Bradford S.Y.C, Mayasundari A, Yang L, Chepyala D, Alcock L, Roberts K.G, Nithianantham S, Maxwell D, Rowland L, Larsen R, Seth A, Goto H, Imamura T, Akahane K & Mullighan C.G. Degradation of Janus Kinase CRLF2-rearranged Acute Lymphoblastic Leukemia. Blood. 2021; 138(23):2301-2302




DOI: https://doi.org/10.24198/ijbp.v3i3.45685

DOI (PDF): https://doi.org/10.24198/ijbp.v3i3.45685.g22261

Refbacks

  • Saat ini tidak ada refbacks.


##submission.license.cc.by-nc4.footer##

IJBP by Universitas Padjadjaran is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License