Cytotoxic Activity and Phytochemical Analysis of Breynia cernua from Papua

Septriyanto Dirgantara; Rosye H.R. Tanjung; Hendra K. Maury; Edy Meiyanto

doi:10.24198/ijpst.v1i1.16121

Cytotoxic Activity and Phytochemical Analysis of Breynia cernua from Papua

Septriyanto Dirgantara, Rosye H.R. Tanjung, Hendra K. Maury, Edy Meiyanto

Abstrak

Breynia cernua (local name Katuk Hutan) is an Indonesian medicinal plant (family: Euphorbiace- ae) originated from Papua which have been used traditionally as alternative treatment for breast and cervical cancer. The objectives of this research were to investigate potential cytotoxic activity and phytochemical aspect test of B. cernua extract and its fractions. Extraction was performed by macera- tion using ethanol 96%, followed by successive fractionations. Phytochemical screening and in vitro cytotoxic activity test were obtained for extract and active fraction with Brine Shrimp Lethality Test (BSLT) method and MCF-7 tumor cell lines were assessed by 3-(4-5-dimethylthiazol-2yl)-2,5-diphe- nyl tetrazolium bromide (MTT) assay. The results from cytotoxic activity test of breast cancer MCF-7 cell line from ethanolic 96% extract and three fractions: n-hexane, ethylacetate and water fractions showed 246.841 ppm, 165.65 ppm, 562.57 ppm dan 713.78 ppm respectively meanwhile the IC50 value of doxorubicin as the positive control was 6 μM. TLC and phytochemical screening showed the pres- ence of alkaloids, terpenoid, avonoids and tannins compounds in this active plant.

Keywords: Antitumor, Breynia cernua, BSLT, MCF-7

Teks Lengkap:

PDF

Referensi

Moelyono, MW. Etnofarmasi. Yogyakar- ta : Deepublish. 2014, 208-213

CDC. Statistics for Different Kinds of Cancer [diunduh 29 Agustus 2017]. Ter- sedia dari: http://www.cdc.gov/ cancer/ dcpc/data/types.htm

Kementerian Kesehatan Republik Indo- nesia.2014. Diakses pada tanggal 9 Sep- tember2017.tersedia dari http :// www. depkes.go.id/resources/download/gener- al/Hasil%20Riskesdas%202013.pdf

Fitriyanti, Wathan, N., Gunawan. Kajian Farmakognostik Tumbuhan Sugi-sugi (Breynia cernua Muel. Arg.) Asal Amun- tai Kalimantan Selatan. Journal Pharmas- cience. 2016;3(2): 43-48.

Khan, M.R. & Omoloso, AD. Antibacterial and Antifungal Activities of Brey- nia cernua. 2008. Journal Fitoterapia. doi:10.1016/j. tote.2008.02.008

Farnsworth, N.R. Biological and Phyto- chemical Screenings of Plant. J. Pharm. Sci. 1966;55(3):225-265.

Meyer, B.N., Ferigni, N.R., Putnam, J.E., Jaconsen, L.B., Nichols, D.F., and Mclaughlin, J.L. Brine Shimp A Con- venient General Bioassay for Active Plant Constituents. Planta Medica. 1982;45:31-34

Meiyanto, E., Putri, DDP, Susidarti RA, Murwanti R., Sardjiman., Fitriasari A, Husnaa U, Purnomo H, Kawaichi M. Curcumin and its Analogues (PGV-0 and PGV-1) Enhance Sensitivity of Resistant MCF-7 Cells to Doxorubicin through In- hibition of HER2 and NF-kB Activation. Asian Pac J Cancer Prev. 2014;15(1): 179-184.

Bruton L, Lazo JS, and Parker KL. Good- man & Gilman’s The Pharmacologi- cal Basis of Therapeutics, 11th Edition, McGrawHill, Lange. 2005.

DOI: https://doi.org/10.24198/ijpst.v1i1.16121