Effect of Ethanol Concentration on Antibacterial Activity of Bligo Fruit Extract (Benincasa hispida Thunb) to Salmonella typhi

Fhahri Mubarak; Sartini Sartini; Dia Purnawanti

doi:10.24198/ijpst.v5i3.16444

Effect of Ethanol Concentration on Antibacterial Activity of Bligo Fruit Extract (Benincasa hispida Thunb) to Salmonella typhi

Fhahri Mubarak, Sartini Sartini, Dia Purnawanti

Abstrak

Bligo fruit (Benincasa hispida Thunb) is one of medicinal plants that used to cure typhoid fever which caused by Salmonella typhi bacteria. The aim of this research is to find ont the effect of ethanol concentration as a solvent from Bligo fruit (Benincasa hispida Thunb) extracts as an antibacterial, especially to Salmonella typhi. This simplicia was being extracted with 96% ethanol, 70% ethanol, and 50% ethanol by using maceration method for 3x24 hours. The antibacterial activity test was done by diffusion of agar method to the MHA medium by obserring the resistor zone which formed from 96% ethanol, 70% ethanol and 50% ethanol. The diametres that have been obtained are 6,815 mm for 96% ethanol, 25,223 mm for 70% ethanol while for 50% ethanol there is no resisten zone can be seen. From the result canbe conclude that the best concentration of ethanol that has a great potencial as resistor to Salmonella typhi bacteria is the Bligo fruit (Benincasa hispida Thunb) extracts of 70% ethanol.

Key words: Bligo fruit (Benincasa hispida Thunb), Antibacterial, Salmonella typhi.

Teks Lengkap:

PDF

Referensi

Abro AH, Abdou AMS, Gangwani JL, Younis NJ, Hussaini HS. 2009. Hematological and biochemical changes in typhoid fever. Pak J Med Sci; 25(2): 166-171

Nasruddin, Hadi U, Vitanata, Erwin AT, Bramantono, Suharto. 2007. Penyakit infeksi diindonesia, Airlangga University Press ; Surabaya.

Raffatellu, M. 2008. Clinical Pathogenesis of Typhoid Fever. J Infect Developing Countries, 2 (4), 260-266.

Madigan, marthinko., stahl and clark. 2012. Biology of Microorganism. Pearson Education, Inc. San Franscisco.

Lecas, L. 2010. Journal of American Medical Association, Antibiotic Resistance a Major Public Health Problem

Esmail, A. 2013. The Pharmacological Importace of Benincasa hispida. A review. Departemen of Pharmacology, Collage of Medicine, University Iraq. ISSN : 0975-9492 Hal 165

Musmuallim, 2006, Isolasi dan identifikasi komponen kimia buah bligo (benincasa hispida) dalam ekstrak etanol, FMIPA; Surakarta.

Marjoni, R., M.P Sari., Y. Lukmayani., L. Syafnir. 2016. Isolasi dan Identifikasi Senyawa Flavonoid pada Tangkai Daun Tembakau (Nicotiana tabacum L). Proceeding of Pharmacy gel 2 Th Akad 2016-2017. SPeSIA . Unisba

Reski, K. 2016. Uji Daya Hambat dan Analisis KLT-Bioautografi Ekstrak Etanol Buah Bligo (Benincasa hispida Thunb) Terhadap Bakteri Uji Salmonella typhi dan Staphylococcus aureus, STIFA Kebangsaan; Makassar.

Oxoid. Muller Hinton Agar, (online) diakses 21 Juni 2017. http://www.oxoid.co

DOI: https://doi.org/10.24198/ijpst.v5i3.16444