Biodistribution of 99mTc-HSA-nanoparticle in Lymphatic Node of Animal Model Induced by 7,12 Dimethylbenz(a)anthracene

Rizky Juwita Sugiharti; Iim Halimah; Isa Mahendra; Witri Nuraeni; Maula Eka Sriyani; Eva Maria Widyasari

doi:10.24198/ijpst.v1i1.19146

Biodistribution of 99mTc-HSA-nanoparticle in Lymphatic Node of Animal Model Induced by 7,12 Dimethylbenz(a)anthracene

Rizky Juwita Sugiharti, Iim Halimah, Isa Mahendra, Witri Nuraeni, Maula Eka Sriyani, Eva Maria Widyasari

Abstrak

Breast cancer is one of the most common malignant tumors found in women and hasbecome as the second cause of death in women after cervical cancer.We successfully synthesizedtechnetium-99m labeled Human Serum Albumin nanoparticle(^99mTc-HSA nanoparticle); a radiopharmaceuticalwhich potentially used for application in lymphoscintigraphytechnique for tracing lymphatic system.This study was aims to obtain pre-clinical data from ^99mTc-HSA nanocolloidin Sprague Dawleyfemale ratsgiven7, 12-dimethylbenzeneanthracene (DMBA) - induced cell proliferation in the breast tissues.The rats are divided into two groups, one groupsaregiven DMBA and observed for 5, 8 and 12 weeks and the other group is without any treatment as control.Biodistribution study of ^99mTc-HSA nanoparticlewas conducted to identify the accumulation of this agent in the popliteal and lumbar lymph nodes and the other tissuecompare to normal rats. ^99mTc-HSA nanoparticleshown good accumulation in popliteal lymph node 2.90+1.32 %ID; 0.81 + 0.47 %IDand 1.04+0.46 %ID at one hour post injectionin 5, 8 and 12 weeks respectively.The popliteal extraction (PE) was estimated with value 87.92 %, 74.07%, 71.79% in 5, 8 and 12 weeks respectively. This result showed that^99mTc-HSA nanoparticleis very promising compound to be lymphoscintigraphyagentin detection of breast cancer.

Key words: lymphoscintigraphy, ^99mTc-HSA nanoparticle, breast cancer

Teks Lengkap:

PDF (English)

Referensi

Departemen Kesehatan Indonesia. 2013. Laporan Hasil Riset Kesehatan Dasar (RISKESDAS) Nasional: Jakarta. Tersedia [Online]: http://www.depkes.go.id/resources/download/general/Hasil%20Riskesdas%2013.pdf

International Atomic Energy Agency. Radiopharmaceuticals for Sentinel Lymph Node Detection: Status and Trends. IAEA Hum Heal Ser. (6).Vienna:International Atomic Energy Agency;2015.

Rosso, K.J. and Nathanson, S.D. Techniques that accurately identify the sentinel lymph node in cancer. World J Surg Proced. 2015;5(1):14-26.

Núñez, E.G.F., Teodoro, R., Wiecek, D.P., Silva, N.G., Martinelli, J.R and Filho, R.S.O. Size and specificity of radiopharmaceuticals for sentinel lymph node detection. Acta Radiologica.2011;52:774-778.

Takeuchi, H., Kitajima, M., and Kitagawa, Y. Sentinel lymph node as a target of molecular diagnosis of lymphatic micrometastasis and local immunoresponse to malignant cells. Cancer Sci. 2008;99(3):441-450.

Devaki Shilpa Surasi, Janis O’Malley, and Pradeep Bhambhvani, 99mTc-Tilmanocept: A Novel Molecular Agent for Lymphatic Mapping and Sentinel Lymph Node Localization, J Nucl Med Technol 2015; 43:87–91

You-Jung Yang, Sung-Jig Li, Jeong-Yoon Song, Tc-99m Diphosphonate as a Potential Radiotracer to Detect Sentinel Lymph Nodes in Patients with Breast Cancer, Nucl Med Mol Imaging (2010) 44:62–68

G.M.M. Gommansa, E.Gommans F.M.vander Zant, G.J.J.Teule T.G.vander Schors, J.W.D.deWaard, 99mTc-Nanocoll : A radiopharmaceutical for sentinel node localisation in breast cancer - In vitro and in vivo results, Appl Radiat Isot. 2009 Sep;67(9):1550-8

Yamaguchi, A., Hanaoka, H., Pirmettis, I., Uehara, T., Tsushima, Y., Papadopoulus, M., and Arano, Y. Injection site radioactivity of 99mTc-labeled mannosylated dextran for sentinel lymph node mapping. Molecular Pharmaceutics.2015;12: 514-519.

Abul K. Azad, Murugesan V. S. Rajaram,Wendy L. Metz, Frederick O. Cope, Michael S. Blue, David R. Vera, and Larry S. Schlesinger, γ-Tilmanocept, a New Radiopharmaceutical Tracer for Cancer Sentinel Lymph Nodes, Binds to the Mannose Receptor (CD206), J Immunol 2015; 195:2019-2029;

Morais, M., Subramanian, S., Pandey, U.C., Samuel, G.V., Venkatesh, M., Martins, M.B., Pereira, S.M., Correia, J.D.G., and Santos, I. Mannosylated dextran derivatives labeled with fac-[M(CO)₃]+ (M = 99mTc, Re) for specific targeting of sentinel lymph node. Mol. Pharmaceutics. 2011;8:609-620.

DOI: https://doi.org/10.24198/ijpst.v1i1.19146