19 Molecular Docking Analysis of Volatile Compounds from Fraction of Eichhornia crassipes Herbs Ethanol Extract As α-Glucosidase Inhibitor

Diniatik Diniatik; Eka Retnowati; Asmiyenti D. Djalil

doi:10.24198/ijpst.v10i1.32399

19 Molecular Docking Analysis of Volatile Compounds from Fraction of Eichhornia crassipes Herbs Ethanol Extract As α-Glucosidase Inhibitor

Diniatik Diniatik, Eka Retnowati, Asmiyenti D. Djalil

Abstrak

Diabetes mellitus is a degenerative disease characterized by an increase in blood sugar (hyperglycemia).
Inhibiting it will prevent the uptake of glucose in the plasma. One of the plants that can be used
as antidiabetic is E. crassipes herbs. This research aims to know the interaction between volatile
chemical compounds from ethyl acetate fraction and ethanol fraction of ethanol extract of E. crassipes
herbs with the α-glucosidase enzyme. This study used ethanol extract of E. crassipes herbs with a
variation of ethyl acetate fraction and ethanol fraction then analyzed the fraction using GC-MS (Gas
Chromatography-Mass Spectroscopy). The ethyl acetate fraction contained 2-pyridine-propanoic
acid, alpha.-methyl-.beta.-oxo-,Ethyl ester;1-propanol-1-di; carbamic acid, methyl ester; acetic acid,
aminooxy-, hydrazide; (2R) - [2-2H2] glycine methyl ester hydrochloride. The ethanol fraction
contained semicarbazide hydrochloride; carbonic dihydrazide; hydroxy-acetaldehyde; 1-propanol-1-
di; acetic acid, and hydroxyl. The result of docking with the α-glucosidase enzyme is 2-pyridinepropanoic
acid, alpha.-methyl-.beta.-oxo-, the Ethyl ester of the ethyl acetate fraction having ΔG is
-4.14 Kcal/mol. Semicarbazide hydrochloride from the ethanol fraction has ΔG of -3.17 Kcal/mol.

Keywords: Diabetes mellitus, docking molecular, (Eichhornia crassipes (Mart.) Solms Herbs, ethyl
acetate fraction, α-glucosidase enzyme.

Kata Kunci

Diabetes mellitus, docking molecular, (Eichhornia crassipes (Mart.) Solms Herbs, ethyl acetate fraction, α-glucosidase enzyme.

Teks Lengkap:

PDF

Referensi

Poretsky, L. Principles of Diabetes Mellitus, Second Edition. New York: Springer. 2017.

Pahlevi, R. Jumlah Penderita Diabetes Indonesia Terbesar Kelima di Dunia. 2021: p. 1. https://databoks.katadata. co.id/datapublish/2021/11/22/jumlah- penderita-diabetes-indonesia-terbesar- kelima-di-dunia

Indrasari, S. D. Hubungan antara diabetes melitus dengan penyakit periodontal. CDK-210. 2013; 40(11): 868-869.

Syarif, A., Purwantyastuti, A., Ari, E., Rianto, S., Arini, S., Armen, M. Farmakologi dan Terapi, Edisi 5. Jakarta: Departemen Farmakologi dan Terapeutik, FKUI. 2016.

Muladi, S. Kajian Eceng Gondok sebagai Bahan Baku Industri dan Penyelamat Lingkungan Hidup di Perairan. Samarinda: Prosiding Seminar Nasional IV Masyarakat Peneliti Kayu Indonesia (MAPEKI). 2001.

Rorong J. A. dan Suryanto E. Analisis fitokimia eceng gondok (Eichhornia crassipes) dan efeknya sebagai agen photoreduksi Fe3+. Chem Prog. 2010; 3(1): 33-41.

Lathitha, P., Shubashini, S. dan Ponnusamy, J. Secondary metabolites of

Eichhornia crassipes (water hyacinth): a review (1949 to 2011). Natural Product

Communications. 2012; 7(9): 1249–1256.

Tyagi, T. dan Mala, A. Phytochemical screening and GC-MS analysisofbioactive constituents in the electrolic extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms. Journal of Pharmacognosy and Phytochemistry. 2017; 6(1): 195-206.

Widyawati, P. S., Budianta, T. D. W., Kusuma, F.A., Wijaya, E. L. The difference of solvent polarity to phytochemical content and antioxidant activity of pluchea indicia less leaves extracts. International Journal of Pharmacognosy and Phytochemical Research. 2015; 6(4): 850-855.

Wijaya, D., Purnama, Y. P., Setya, A. R. dan Rizal, M. Screening fitokimia dan aktivitas antioksidan daun eceng gondok (Eichhornia crassipes). Jurnal Kimia Valensi. 2015; 1(1): 65-69.

Meydia, Suwandi, R. dan Suptijah, P. Isolasi senyawa steroid dari teripang gama (Stichopus variegatus) dengan berbagai jenis pelarut. JPHPI. 2016; 19(3): 562- 369.

Male, Y. J., Wayan, S., dan Olivia, M. R. Komputasi zat warna (dyes) alami sebagai material aktif pada sel surya organic menggunakan teori fungsional kerapatan (Density Functional Theory, DFT). Ind. J. Chem. Res. 2015; 2: 205-212.

Sim, L., Roberto, Q. C., Erwin, E. S., Buford, L. N., dan David R. R. Human intestinal maltase–glucoamylase: crystal structure of the n-terminal catalytic subunit and basis of inhibition and substrate specificity. J. Mol. Biol. 2008; 375: 782–792.

Setiawan ,T. Studi molecular docking ekstrak kurkuminoid asal wonogiri sebagai inhibitor enzim dna topoisomerase II. Tesis. Bogor: Institute Pertanian Bogor. 2015: p. 1-54.

Priyoherianto, A., Suci, P.R., Putri Rizki Cahya Fatimah, P.R.C., Wijayanti, A.N. Uji Aktivitas Antidiabetes Kombinasi Ekstrak Etanol Daun Eceng Gondok

(Eichhornia crassipes (Mart.)Solms) Dan Daun Sintrong (Crassocephalum crepidioides (Benth.) S. Moore) Pada Mencit, Jurnal Insan Farmasi Indonesia. 2021; 4(1): 44-53

Ahmed, R., Mustofa, M.N., & Rahmatullah. Oral glucose Tolerance Test (OGTT) With ACombination of Colocasia Esculenta Stems and Eichhornia crassipes Aerial Parts, World Journal of Pharmacy and Pharmaceutical Sciences. 2018; 7(1): 207-2014

Krismariono, A., Setiawatie, E.M., Rachmawati, R.Y., Setiawan, Y.A., Padmarin, H.N., Apriliyanti, N,A., Antibacterial Activity of Water Hyacinth (Eichhornia Crassipes) Leaf Extract Against Bacterial Plaque from Gingivitis Patients, Journal of International Dental and Medical Research, 2022; 15(3): 966- 917

Su, W., Sun, Q., Xia, M., Wen, Z. and Yao, Z. The Resource utilization of Water Hyacinth (Eichhornia crassipes (Mart.) Solms) and its Challenges. Resources. 2018; 7 (46):2-9.

Ilo, O.P., Simetele, M.D., Nikomo, S.L., Mkhize, N.M., Prabhu, N.G. The Benefits of Water Hyacinth (Eichhornia crassipes (Mart.) Solms) for Southern Africa: A Review. Sustainability. 2020; 12 (9222): 2-20.

Ting, W.H.T., Salleh, I.A.W., Wahab, N.A. Application of Water hyacinth (Eichhornia crassipes) for Phytoremidiation of Ammonical Nitrogen: A. Review. Journal od Water Process Engineering, 2018; 22:239-249.

DOI: https://doi.org/10.24198/ijpst.v10i1.32399