Authentication	of	Lemongrass	Oil	By	Gas	Chromatography-Mass Spectroscopy (GC-MS) Combination Chemometrics

Any Guntarti; Husnun Khairunnisa Pratiwi; Laela Hayu Nurani; Ibnu Gholib Gandjar

doi:10.24198/ijpst.v9i3.32558

Authentication of Lemongrass Oil By Gas Chromatography-Mass Spectroscopy (GC-MS) Combination Chemometrics

Any Guntarti, Husnun Khairunnisa Pratiwi, Laela Hayu Nurani, Ibnu Gholib Gandjar

Abstrak

The addition of something foreign to lemongrass essential oil has been done since ancient times. One way is to add turpentine oil which aims to increase the volume and grade of the smell. The purpose of this study was to determine the authentication of citronella oil using the gas chromatography-mass spectrometry (GC-MS) method combined with multivariate PCA analysis. Fragrant lemongrass oil was isolated by distillation of water and using simplicia in the form of powdered dried Cymbpogon citratus leaves. The oil obtained was treated with anhydrous Na2SO4. The results of the characteristics and quality requirements of citronella oil are compared with the SNI. The results of GC-MS analysis were the relative levels of essential oils. The results of the characteristic test meet the quality requirements of SNI 06-3953-1995, except for the yield requirements. The results of analysis by GC-MS, the essential content of citronella oil was citronella (37.47%), geraniol (25.03%), trans-caryophilene (3.69%), citronellol (11.68%), and delta cadinene. (2.44%). Chemometric principle component analysis (PCA) can group samples of fragrant lemongrass oil, turpentine oil and lemongrass oil products on the market.

Kata Kunci

citronella oil, GC- MS, PCA, chemometric.

Teks Lengkap:

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Referensi

Shafirany MZ, Yasmiwar S, dan Ida M. Aplikasi kemometrik dalam penentuan mutu tumbuhan obat. Pharmauho;2018(4):2 p 6-14

Bota W, Martanto M, dan Ferdy SR. Karakterisasi produk-produk minyak sereh wangi (citronella oil) menggunakan spektroskopi inframerah dekat (NIRs).

Prosiding. Jakarta: Seminar Nasional Sains dan Teknologi Fakultas Teknik Universitas Muhammadiyah Jakarta; 2015.

Sulaswatty A, Meika SR, Haznan A, and Silvester T. Quo vadis minyak atsiri serai dan turunannya. Jakarta : LIPI Press; 2017.

Dutta, D.; Kumar, P.; Nath, A.; Verma, N.; Gangwar, B. Qualities of lemongrass (Cymbopogan citratus) essential oil at different drying conditions. Inter J Agri Env Biotech. 2014; 7: 903-909, doi:10.5958/2230-732X.2014.01403.X.

Feriyanto YE, Patar JS, Mahfud, dan Pantjarni P. Pengambilan minyak atsiri daun dan batang serai wangi (Cymbopogon winterianus) menggunakan metode destilasi uap dan air dengan pemanasan microwave. J Tek POMITS 2016;(2)1: 2337 – 3539

Ma’mun. Identifikasi pemalsuan minyak nilam di rantai tataniaga. J Hortikul. 2003;14(2):17-22

Bazakos, Christos, Spaniolas, Stelios, Kalaitzis, Panagiotis. "DNA-Based Approaches for Traceability and Authentication of Olive Oil". Products from Olive Tree, edited by Dimitrios Boskou, Maria Clodoveo, IntechOpen, 2016. 10.5772/64494.

Rohman A. Anzar W, Sugeng R, Sudjadi, Shahrel A, Arieff SR, and Farahwahida MY. Fourier transform infrared spectroscopy combined with multivariate calibrations for the authentication of avocado oil. Inter J Food Prop. 2016;(19)

: 680—687.

Muchtaridi, M.; Musfiroh, I.; Subarnas,A.; Rambia, I.; Suganda, H.; Nasrudin, M.E. Chemical composition and locomotors activity of essential oils from the rhizome, stem, and leaf of Alpinia malaccencis (Burm F.) of Indonesian Spices. Journal of Applied Pharmaceutical Science 2014, 4, 052-056.

Lobb K, Chow, CK. Fatty Acid Classification and Nomenclature.USA: Fatty Acid in Foods and Their Health Implications. 3rd Edition, Florida 2018:1296;

Miller, JN, Miller, JC. Statistics and Chemometrics for Analytical Chemistry. 6th ed. Harlow, England: Pearson Education Limited, 296 p; 2013

Nahor, EM, Benedicta, IR, dan Hesti YT. Perbandingan randemen ekstrak etanol daun andong menggunakan ekstraksi maserasi dan sokletasi. Semarang: Prosiding: Interprofessional Colaboration Dalam Pengembangan Center of Exellence Inovasi Pangan Lokal pada Era New Normal. 2020: 40-44

Rohman A, Man, YBC. The chemometrics approach applied to FTIR spectral data for the analysis of rice bran oil in extra virgin olive oil. Chemometrics and Intelligent Laboratory Systems. Chemometric and Intelligent Laboratory Systems 2012;(110):1 p 129-134

Hotmian E, Elly S, Fatimawali, dan Trina

T. Analisis GC-MS ekstrak etanol umbi rumput teki. Manado: Pharmacon, FMIPA Universitas Sam Ratulangi 2021;(10)

Wei X, Koo I, Kim S, Zhang X. Compound identification in GC-MS by simultaneously evaluating the mass spectrum and retention index. Analyst. 2014 139(10):2507-14.

Eden WT, Alighiri E. Cahyono, Supardi, and Wijayanti. Fractionation of Java citronella oil and citronellal purification by batch vacuum fractional distillation. IOP Conf Chem Series. The 12th Joint Conference on Chemistry. 2018. 349:12067.

Wany A, Shivesh J, Vinod KN, and Dev MP. Chemical Analysis and Therapeutic Uses of Citronella Oil From Cymbopogon winterianus : A Short Review. Inter J Adv Res. 2017;(1): 504-521.

Putri, AR, Abdul R, Sugeng R, Widiastuti

S. Autentikasi minyak ikan patin menggunakan metode spektrofotometri FTIR yang dikombinasikan dengan kemometrika. Indo J Chemomet Pharm Anal. 2021. 1(1): 22-27

Rohman A. Anzar W, Sugeng R, Sudjadi, Shahrel A, Arieff SR, and Farahwahida MY. Fourier transform infrared

spectroscopy combined with multivariate calibrations for the authentication of avocado oil. Inter J Food Prop. 2016;(19)

: 680—687.

Rohman, A. Physico-chemical properties, biological activities and authentication of cod liver oil. J Food Pharm Sci. 2017; (5):1-7

Zhao Q, Yang K, Li W,Xing B. Concentra- tion-depenent polyparameter linear free energy relationships to predict organic compound sorption on carbon nanotubes. Scientific Reports. 2014;(4):3888-9

DOI: https://doi.org/10.24198/ijpst.v9i3.32558