Radiochemical Purity Test of Fractionated Sestamibi Kit Labelled with Technetium-99m

Hasna Chaerunisa; N. Elly Rosilawati; Muchtaridi Muchtaridi

doi:10.24198/ijpst.v1i1.33332

Radiochemical Purity Test of Fractionated Sestamibi Kit Labelled with Technetium-99m

Hasna Chaerunisa, N. Elly Rosilawati, Muchtaridi Muchtaridi

Abstrak

Coronary Heart Disease (CHD) is a heart disorder caused by blockage of blood vessels. CHD can be
detected by Myocardial Perfusion Imaging (MPI). MPI is performed by injecting a radiopharmaceutical
into the patient's body. 99mTc-sestamibi is a radiopharmaceutical that is commonly used in MPI.
Sestamibi is available in the form of a multidose vial, but the cost of the examination will be expensive
if it is only used for one patient. Cost effectiveness can be increased by fractionating the sestamibi kit
before labelled by 99mTc. 99mTc-sestamibi needs to be tested for quality control before it is administered
to the patients. One of the tests is the radiochemical purity test. The aim of this study was to determine
the radiochemical purity of the fractionated sestamibi kit labelled by 50 mCi 99mTc. 2 vials of sestamibi
kit were fractionated by adding 5 mL of 0.9% NaCl solution to each vial and divided into 10 new vials.
Radiochemical purity was measured using the thin layer chromatography (TLC) method. The results
of this study indicated that all samples had radiochemical purity of 100% up to 6 hours after labelling.

Kata Kunci

Radiopharmaceutical, 99mTc-sestamibi, fractionation, radiochemical purity.

Teks Lengkap:

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Referensi

Daftar Pustaka

Kemenkes. Info Datin : Situasi Kesehatan Jantung. Jakarta: Kemenkes RI; 2014.

Vlodaver Z, Wilson RF, Garry DJ. Coronary Heart Disease : Clinical, Pathological, Imaging, and Molecular Profiles. New York: Springer; 2012.

Doroudi A, Saraji F, Erfani M, Saadati SM, Kisast A, Ahmadi F, et al. The stability of 99mTc-MIBI (Sestamibi) complex samples which prepared under ultrasound irradiation technique versus boiling water bath method. J Appl Pharm Sci. 2016;6(11):126–34.

Boschi A, Uccelli L, Martini P. A Picture of Modern Tc-99m Radiopharmaceuticals: Production, Chemistry, and Applications in Molecular Imaging. Appl Sci. 2019;9(2526).

NCBI. PubChem Compound Summary for CID 9543089, Pertechnetate [Internet]. 2020 [cited 2020 May 30]. Available from: https://pubchem.ncbi.nlm.nih.gov/ compound /Pertechnetate

Muchtaridi. Dasar-dasar Radiofarmasi : Pengembangan Untuk Diagnosis dan Terapi. Yogyakarta: Deepublish; 2014.

Orsini F, Lorenzoni A, Erba PA, Mariani G. Radiopharmaceuticals for Single Photon Emission Imaging and for Therapy. In: Nuclear Oncology: Pathophysiology and Clinical Applications. New York: Springer Science+Business Media; 2013. p. 21–34.

Vrachimis A, Hermann S, Máthé D, Schober O, Schäfers M. Systematic evaluation of 99mTc-tetrofosmin versus 99mTc-sestamibi to study murine myocardial perfusion in small animal SPECT/CT. EJNMMI Res. 2012;2(1):1– 9.

Daruwati I, Sriyani ME, Oekar NK, Hanafiah A, Zainuddin N. Profile of MIBI liquid phase radiopharmaceutical for myocardial imaging. Atom Indones. 2016;42(1):27–32.

Bucerius J, Ahmadzadehfar H, Biersack H. 99mTc-Sestamibi : Clinical Application. Berlin: Springer Berlin Heidelberg; 2012.

Mansur MS, Mushtaq A, Jehangir M. Fractionation of lyophilized MIBI kit for99mTc labeling. J Radioanal Nucl Chem. 2006;268(1):141–3.

Matsuo S, Nakajima K, Kinuya S. Evaluation of Cardiac Mitochondrial Function by a Nuclear Imaging Technique using Technetium-99m-MIBI Uptake Kinetics. Asia Ocean J Nucl Med Biol. 2013;1(1):39–43.

Saha GB. Fundamentals of Nuclear Pharmacy. 7th ed. Ohio: Springer; 2018.

Muchtaridi M, Setyawati LU, Islamiaty RR, Lie KR, Nurhidayah W. The purity identification and radiolabeling of α-mangostin with technetium-99m. J Adv Pharm Technol Res. 2020;11(1):6–12.

Zolle I. Technetium-99m Pharmaceuticals : Preparation and Quality Control Nuclear Medicine. Berlin: Springer; 2007.

Jeon SJ, Kim KM, Lim I, Song K, Kim JG. Pixelated scintillator-based compact radio thin layer chromatography scanner for radiopharmaceuticals quality control. J Instrum. 2017;12(11).

Wang J, Rios A, Lisova K, Slavik R, Chatziioannou AF, van Dam RM. High-throughput radio-TLC analysis. Nucl Med Biol [Internet]. 2020;82–83:41–8. Available from: https://doi.org/10.1016/j. nucmedbio.2019.12.003

Council of Europe. European Pharmacopoeia. Strasbourg: Council of Europe; 2019.

Ziessman HA, O’Malley JP, Thrall JH. Radiopharmaceuticals. In: Nuclear Medicine. Philadelphia: Elsevier Inc.; 2014. 1–15 p.

Gilmore GR. Practical Gamma-ray Spectrometry. 2nd ed. Warrington: John Wiley & Sons; 2008.

Boria AJ, Rucinski BD, Abraham SA, Dawson AS, Jawad AH, Miklos JA, et al. 137Cs Dosimeter Irradiation Facilities: Calibration Frequency, Precision, and Accuracy. Health Phys. 2017;112(4):357– 63.

POLATOM. PoltechMIBI [Internet]. 2018 [cited 2021 Jan 20]. Available from: https://www.polatom.pl/sites/default/ files/2018.02.09 PoltechMIBI SPCh-A2 zmniejszyc do A3.pdf

DOI: https://doi.org/10.24198/ijpst.v1i1.33332