Potency of Honje Hutan Flowers (Etlingera Hemisphaerica (Blume) R.M.Sm.) as Alpha-Glucosidase Inhibitor

Soraya Riyanti; Nandi Agustian; Akhirul Kahfi Syam

doi:10.24198/ijpst.v10i1.35453

Potency of Honje Hutan Flowers (Etlingera Hemisphaerica (Blume) R.M.Sm.) as Alpha-Glucosidase Inhibitor

Soraya Riyanti, Nandi Agustian, Akhirul Kahfi Syam

Abstrak

The Pangandaran people process honje flowers into fresh drinks that are consumed for health. The
forest honje leaf extract provides antidiabetic activity at a dose of 0.39 mg/g BW can reduce blood
glucose levels by 36.16% has been reported. Limited scientific information from honje hutan flowers,
it is necessary on its activity as an alpha-glucosidase inhibitor and study its chemical content. The
extraction process was macerated in 70% ethanol for 24 hours and the fractionation process was
carried out by liquid-liquid extraction method with n-hexane, ethyl acetate and water as solvents.
Testing the activity of alpha-glucosidase inhibitors colorimetrically using the chromogenic substrate
p-nitrophenyl α-D-glucopyranoside, alpha-glucosidase enzymes derived from Saccharomyces
cerevisiae, measurement of absorption at a maximum wavelength of 401.3 nm and acarbose used
as a comparison drug alpha-glucosidase inhibitors. The results showed that the forest honje flowers
contain flavonoids, polyphenols, tannins, quinones, monoterpenoids, and sesquiterpenoids. IC50 values
for 70% ethanol extract, n-hexane, ethyl acetate, water and acarbose fractions were 136.79, 307.18,
277.12, 66.18, and 14.63 g/mL respectively. The water fraction gave the best inhibitory activity against
alpha-glucosidase with an IC50 value of 66.18 g/mL.

Keywords: Etlingera hemisphaerica (Blume) R.M.Sm, honje hutan flowers, alpha-glucosidase
inhibitor.

Kata Kunci

Etlingera hemisphaerica (Blume) R.M.Sm, honje hutan flowers, alpha-glucosidase inhibitor.

Teks Lengkap:

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Referensi

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DOI: https://doi.org/10.24198/ijpst.v10i1.35453