Indonesian Journal of Pharmaceutical Science and Technology
Evaluation of Antioxidant Activity of Vernonia amygdalina Leaves and Its Flavonoid-Phenolic Content
Abstrak
Bitter leaf, Vernonia amygdalina (VA), is used in various alternative medicine in several countries,
including Indonesia. This study aims to evaluate the antioxidant activity of VA leaves by three different
in vitro methods. The antioxidant activity was evaluated by radical scavenging of 1,1-diphenyl-2-
picryl-hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant
capacity (CUPRAC). The antioxidant capacity by FRAP and CUPRAC methods were expressed as
mg of quercetin equivalents (QE) per g extract (mgQE/g) and mg of ascorbic acid equivalents (AAE)
per g extract (mgAAE/g), respectively. All methods were measured by UV-Visible spectrophotometry
at maximum wavelength. The results exhibited that VA leaves ethanolic extract contains flavonoidphenolic.
The VA leaves ethanolic extract has an antioxidant activity by scavenging the DPPH method
with the half inhibition concentration (IC50) was 8,22 μg/mL. The antioxidant capacity of VA leaves
ethanolic extract by FRAP and CUPRAC methods was 21,03 mgQE/g and 160,64 mgAAE/g extract,
respectively. The VA leaves ethanolic extract has a powerful antioxidant potency which might correlate
to the flavonoid-phenolic content in the extract.
Kata Kunci
Teks Lengkap:
Tidak berjudul (English)Referensi
Di Meo S, Venditti P. Evolution of the Knowledge of Free Radicals and Other Oxidants. Oxidative Medicine and Cellular Longevity. 2020; 2020: 9829176.
Phaniendra A, Jestadi DB, Periyasamy L. Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian Journal of Clinical Biochemistry. 2015; 30: 11-26.
Özyürek M, Güçlü K, Tütem E, et al. A comprehensive review of CUPRAC methodology. Analytical Methods. 2011; 3: 2439-53.
Oyeyemi IT, Akinlabi AA, Adewumi A, et al. Vernonia amygdalina: A folkloric herb with anthelminthic properties. Beni-Suef University Journal of Basic and Applied Sciences. 2018; 7: 43-49.
Alara OR, Abdurahman NH, Ukaegbu CI, et al. Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques. Journal of Taibah University for Science. 2019; 13: 414-22.
Fawwaz M, Pratama M, Hasrawati A, et al. Total Carotenoids, Antioxidant and Anticancer Effect of Penaeus monodon Shells Extract. Biointerface Research in Applied Chemistry. 2021; 11: 11293 - 302.
Liu D, Shi J, Colina Ibarra A, et al. The scavenging capacity and synergistic effects of lycopene, vitamin E, vitamin C, and β-carotene mixtures on the DPPH free radical. LWT - Food Science and Technology. 2008; 41: 1344-49.
Nadri MH, Salim Y, Basar N, et al. Antioxidant activities and tyrosinase inhibition effects of Phaleria macrocarpa extracts. Afr J Tradit Complement Altern Med. 2014; 11: 107-11.
Flieger J, Flieger W, Baj J, et al. Antioxidants: Classification, Natural Sources, Activity/Capacity Measurements, and Usefulness for the Synthesis of Nanoparticles. Materials (Basel). 2021; 14.
Shahidi F, Zhong Y. Measurement of antioxidant activity. Journal of Functional Foods. 2015; 18: 757-81.
Kato S, Aoshima H, Saitoh Y, et al. Highly hydroxylated or γ-cyclodextrin-bicapped water-soluble derivative of fullerene: The antioxidant ability assessed by electron spin resonance method and β-carotene bleaching assay. Bioorganic & Medicinal Chemistry Letters. 2009; 19: 5293-96.
Khiya Z, Oualcadi Y, Gamar A, et al. Correlation of Total Polyphenolic Content with Antioxidant Activity of Hydromethanolic Extract and Their Fractions of the Salvia officinalis Leaves from Different Regions of Morocco. Journal of Chemistry. 2021; 2021: 8585313.
Muflihah YM, Gollavelli G, Ling YC. Correlation Study of Antioxidant Activity with Phenolic and Flavonoid Compounds in 12 Indonesian Indigenous Herbs. Antioxidants (Basel). 2021; 10.
Payne AC, Mazzer A, Clarkson GJ, et al. Antioxidant assays - consistent findings from FRAP and ORAC reveal a negative impact of organic cultivation on antioxidant potential in spinach but not watercress or rocket leaves. Food Sci Nutr. 2013; 1: 439-44.
Kikuzaki H, Hisamoto M, Hirose K, et al. Antioxidant Properties of Ferulic Acid and Its Related Compounds. Journal of Agricultural and Food Chemistry. 2002; 50: 2161-68.
Özkök A, Keskin M, Tanuğur Samancı AE, et al. Determination of antioxidant activity and phenolic compounds for basic standardization of Turkish propolis. Applied Biological Chemistry. 2021; 64: 37.
Apak R, Güçlü K, Ozyürek M, et al. Cupric ion reducing antioxidant capacity assay for antioxidants in human serum and for hydroxyl radical scavengers. Methods Mol Biol. 2010; 594: 215-39.
Trofin AE, Trincă LC, Ungureanu E, et al. CUPRAC Voltammetric Determination of Antioxidant Capacity in Tea Samples by Using Screen-Printed Microelectrodes. Journal of Analytical Methods in Chemistry. 2019; 2019: 8012758.
Martemucci G, Costagliola C, Mariano M, D’andrea L, Napolitano P, D’Alessandro AG. Free Radical Properties, Source and Targets, Antioxidant Consumption and Health. Oxygen. 2022; 2(2):48-78.
Ugbogu EA, Emmanuel O, Dike ED, Agi GO, Ugbogu OC, Ibe C, Iweala EJ. The Phytochemistry, Ethnobotanical, and Pharmacological Potentials of the Medicinal Plant-Vernonia amygdalina L. (bitter Leaf). Clinical Complementary
Medicine and Pharmacology. 2021; 1(1).
DOI: https://doi.org/10.24198/ijpst.v10i2.41617
Refbacks
- Saat ini tidak ada refbacks.
| Switch to English Back to Top |
| View My Stats Penerbit Universitas Padjadjaran
Jurnal ini terindeks di : Creative Commons Attribution :
Based on a work at http://jurnal.unpad.ac.id/ijpst/ |
