Determination of Gamma Oryzanol from Ethanolic Extract of Indonesian Rice Bran (Oryza sativa)

Afifah Vardhani; Hidayah Sunar Perdanastuti

doi:10.24198/ijpst.v11i1.46297

Determination of Gamma Oryzanol from Ethanolic Extract of Indonesian Rice Bran (Oryza sativa)

Afifah Vardhani, Hidayah Sunar Perdanastuti

Abstrak

Rice bran contains gamma oryzanol, a chemical compound mostly consisted of complex ester trans-ferulate with phytosterols1. Gamma oryzanol has a strong antioxidant activity, explained by the free radical scavenging activity. This study was aimed to determine gamma oryzanol from ethanolic extract of rice bran. Quantitative analysis was conducted using HPLC analysis with mixture of methanol:acetonitrile:isopropanol (50%:40%:10%) as mobile phase and Inertsil ODS-3 5 μm as stationary phase. The UV detector wavelength was set to 327 nm, and the flow rate was set to 1 mL/min2. Gamma oryzanol standard and rice bran ethanolic extract 96% was injected into HPLC system. Ethanolic extract of rice bran (Oryza sativa) sample showed four major peaks as those of gamma oryzanol standard and contained 105,981 ppm of gamma oryzanol. As the conclusion, ethanolic extract of rice bran (Oryza sativa) contains gamma oryzanol

Kata Kunci

Rice bran, gamma oryzanol, HPLC

Teks Lengkap:

PDF (English)

Referensi

Calpe, C. Rice International Commodity Profile. Food and Agriculture Organization of the United Nations Markets and Trade Division. 2016.

Ito, V.C., Lacerda, L.G. Black rice (Oryza sativa L.): A review of its historical aspects, chemical composition, nutritional and functional properties, and applications and processing technologies. Food Chemistry. 2019;301.

Jun, H.I., Shin, J.W., Song, G.S., Kim, YS. Isolation and Identification of Phenolic Antioxidants in Black Rice Bran. Food Chemistry. 2015;80(2).

Shammugasamy, B., Ramakrishnan, Y., Manan, F. et al. Rapid Reversed Phase Chromatographic Method for Determination of Eight Vitamin E Isomers and γ-Oryzanols in Rice Bran and Rice Bran Oil. Food Anal. Methods.2015;8:649–55.

Ghasemzadeh, A., Karbalaii, M.T., Jaafar, H.Z.E. et al. Phytochemical constituents, antioxidant activity, and antiproliferative properties of black, red, and brown rice bran. Chemistry Central Journal. 2018;12: 17.

Tsuzuki, W., Komba, S. & Kotake-Nara, E. Diversity in γ-oryzanol profiles of Japanese black-purple rice varieties. J Food Sci Technol. 2019; 56: 2778–86.

Ghatak, S.B., Panchal, S.J. Gamma oryzanol: a multi purpose steryl ferulate. Current Nutrition & Food Science. 2011; 7:10-20.

Francisqueti, F.V., Minatel, I.O., Ferron, A.J.T., Bazan, S.G.Z., Silva, V.D.S., Garcia, J.L., De Campos, D.H.S., Ferreira, A.L., Moreto, F., Cicogna, A.C. et al. Effect of Gamma-Oryzanol as Therapeutic Agent to Prevent Cardiorenal Metabolic Syndrome in Animals Submitted to High Sugar-Fat Diet. Nutrients. 2017;9:1299.

Tsuzuki, W., Komba, S., Kotake-Nara, E. Diversity in γ-oryzanol profiles of Japanese black-purple rice varieties. J Food Sci Technol. 2019;56: 2778–86.

Vardhani, A., Jufri, M., Purwaningsih, E. Potency of γ-oryzanol-rich black rice bran (Oryza Sativa L. Indica) extract for tyrosinase inhibition. Int J Pharm Pharm Sci. 2020;12(5):90-93.

Bessa LCBA, Ferreira MC, Rodrigues CEC, Batista EAC, Meirelles AJA.

Simulation and process design of continuous countercurrent ethanolic extraction of rice bran oil. J Food Eng. 2017

Ruen-ngam, D., Thawai, C., Nokkoul, R., Sukonthamut, S. Gamma-oryzanol extraction from upland rice bran. International Journal of Bioscience, Biochemistry and Bioinformatics. 2014;4(4).

Trinovita, E., Saputri, F.C., Munim, A. Potential gastroprotective activity of rice bran (Oryza sativa L.) extracted by ionic liquid-microwave-assisted extraction against ethanol-induced acute gastric ulcers in rat model. Sci. Pharm. 2018;86,35.

Kumar, P., Yadav, D., Kumar, P. et al. Comparative study on conventional, ultrasonication and microwave assisted extraction of γ-oryzanol from rice bran. J Food Sci Technol. 2016; 53:2047–253.

Garofalo, S.F., Tommasi, T., Fino, D. A short review of green extraction technologies for rice bran oil. Biomass Conv Bioref. 2021;11:569–87.

Srisaipet, A., Nuddagul, M. Influence of temperature on gamma-oryzanol stability of edible rice bran oil during heating. International Journal of Chemical Engineering and Applications. 2014;5(4):303-6.

Imsanguan, P., Roaysubtawee, A., Borirak, R., Pongamphai, S., Douglas, S., Douglas, P.L. Extraction of a-tocopherol and g-oryzanol from rice bran. Food Sci Technol. 2008;41:1417–24.

Lerma-García, M. J., Herrero-Martínez, J. M., Simó-Alfonso, E. F., Mendonça, C. R. B., Ramis-Ramos, G. Composition, industrial processing and applications of rice bran γ-oryzanol. Food Chemistry. 2009;115:389–404.

Xu, Z., Hua, N., Godber, J.S. Antioxidant activity of tocopherols, tocotrienols, and γ-oryzanol components from rice bran against cholesterol oxidation accelerated by 2,20-azobis (2-methylpropionamidine) dihydrochloride. J. Agric. Food Chem, 2001;49:2077–81.

Huang, Y.P., Lai, H.M. Bioactive compounds and antioxidative activity

of colored rice bran. J. Food Drug Anal. 2016; 24:564-74.

DOI: https://doi.org/10.24198/ijpst.v11i1.46297