Nanoemulsion of Nagasari (Mesua ferrea L.) Leaf and Its Activity Against Staphylococus aureus

Tuti Sri Suhesti; Annisa Muslimah

doi:10.24198/ijpst.v12i2.49866

Nanoemulsion of Nagasari (Mesua ferrea L.) Leaf and Its Activity Against Staphylococus aureus

Tuti Sri Suhesti, Annisa Muslimah

Abstrak

Nagasari leaves (Mesua ferrea L.) contain special chemicals to fight S. aureus. Nanoparticle technology can improve the solubility, stability, and bioavailability of the phytochemicals found in herbal medicines. The nanoemulsion produces small particle sizes. It has a large surface area. This research aims to determine the physical stability of Nagasari leaf ethanol extract nanoemulsion (EDN) with variations of Virgin Coconut Oil (VCO) as the oil phase during storage and its activity. Nanoemulsions were produced using 1%, 2%, and 3% VCO concentrations. The physical properties and stability of the preparations were characterised. The nanoemulsion was tested for activity against S. aureus. Chloramphenicol was used as a positive control. The results showed that the nanoemulsion containing 1% VCO produced the best physical properties: a particle size of 23.137 ± 7.63 nm, a polydispersity index (PDI) of 0.203 ± 0.013, and a zeta potential of -18.9 ± 0.086. A VCO concentration of 1% produces good nanoemulsion stability. The F1 nanoemulsion exhibited an average inhibition zone diameter of 16.75 mm (strong), the EDN provided an inhibition zone diameter of 8.70 mm (medium), and the positive control exhibited an inhibition zone diameter of 24.30 mm (very strong).

Kata Kunci

nanoemulsion; nagasari leaf; S. aureus; virgin coconut oil

Teks Lengkap:

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Referensi

Chakraborty D, Arefin P, Bhattacharjee SC, Hasan M, Sarkar R, Das S, Rahman S, Habib MS, Islam S, Jahan F, Ray G, Ferdous J, Farhana F, Islam MA, Mostafa M. Biological activity of Mesua ferrea (Nageswar) seed extracts: An in vitro and in silico study. Informatics Med Unlocked. 2023;36:1-8.

Yuliah Y, Hakim L, Hadiyan Y. Nagasari (Mesua ferrea): Budidaya dan Potensinya sebagai Tanaman Obat. Silviculture and Medicinal Plant Potential. Proceeding Biol Educ Conf. 2018;15 (1):808-812.

Suhesti TS, Rohman MM, Sunarto. Formulation of Gel Hand Sanitizer of Nagasari Leaf Extract (Mesua ferrea L.). IJPST SUPP. 2021;1(1): 31-38.

Prasetyaningrum A, Jos B, Gunadi A, Krisnanda A. The Effect of Ozonation Process on Antibacterial Activity of Carrageena, Advanced Science Letters. 2017;23(6): 5783-5.

Bhosale AP, Patil A, Swami M. Herbosomes As A Novel Drug Delivery System For Absorption Enhancement. World Journal of Pharmacy and Pharmaceutical Sciences. 2015; 5(2): 345-355.

Kumar A, Kumar B, Singh SK, Kaur B, Singh S. A Review on Phytosomes: Novel Approach for Herbal Phytochemicals. Asian Journal of Pharmaceutical and Clinical Reseacrh. 2017;10(10): 41-7.

Jaiswal M, Dudhe R, Sharma PK. Nanoemulsion: An Advanced Mode Of Drug Delivery System. 3 Biotech. 2015; 5(2):123-127.

Listyorini NMD, Wijayanti NLPD, Astuti KW. Optimasi Pembuatan Nanoemulsi Virgin Coconut Oil. Jurnal Kimia. 2018; 12(1):8-12.

Sari AI, Herdiana Y. Formulasi Nanoemulsi Terhadap Peningkatan Kualitas Obat. Review Jurnal, Farmaka. 2018;16(1):247-54.

Xiao M, Luo L, Tang B, Qin J, Wu K, Jiang F. Physical, structural, and water barrier properties of emulsified blend film based on konjac glucomannan/agar/gum Arabic incorporating virgin coconut oil. LWT. 2022;154:112683. doi.org/10.1016/j.lwt.2021.112683

Mu’awanah IAU, Setiaji B, Syoufian A. Pengaruh Konsentrasi Virgin Coconut Oil (VCO) Terhadap Stabilitas Emulsi Kosmetik Dan Nilai Sun Protection Factor (SPF). BIMIPA. 2014;24(1):1-11.

Jusnita N, Syurya W. Karakterisasi Nanoemulsi Ekstrak Daun Kelor (Moringa oleifera Lamk.). Jurnal Sains Farmasi & Klinis. 2019; 6(1): 16-24.

Ayuningtias DDR, Nurahmanto D, Rosyidi VA. Optimasi Komposisi Polietilen Glikol dan Lesitin sebagai Kombinasi Surfaktan pada Sediaan Nanoemulsi Kafein. Pustaka Kesehatan. 2017;5(1): 157-63.

Yuliani SH, Hartini M, Stephanie B, Pudyastuti B, Istyastono EP. Comparison Of Physical Stability Properties Of Pomegranate Seed Oil Nanoemulsion Dosage Forms With Long-Chain Triglyceride And Medium-Chain Triglyceride As The Oil Phase. Majalah Obat radisional, 2016; 21(2): 93-8.

Anindhita MA, Oktaviani N. Formulasi SelfNanoemulsifying Drug Delivery System (SNEDDS) Ekstrak Daun Pepaya (Carica papaya L.) dengan Virgin Coconut Oil (VCO) sebagai Minyak Pembawa. Pena Medika Jurnal Kesehatan, 2016; 6(2).

Laxmi M, Bhardwaj A, Mehta S, Mehta A. Development and Characterization of Nanoemulsion as Carrier for The Enhancement of Bioavailability of Artemether. Artificial Cells, Nanomedicine, and Biotechnology. 2015; 43(5): 334-44.

Zhang XC, Yang XF, Gu TZ, Cao YF, Lu CH. Antimicrobial polycyclic polyprenylated acylphoroglucinols from Mesua ferrea flower. Phytochem Lett. 2020;40(July):84-8.

Pramudono B, Pawignya H, Kusworo TD. Kinetic Modeling of Flocculation and Coalescence in the System Emulsion Water-Xylene-Terbutyl Oleyl Glycosides. Bulletin of Chemical Reaction Engineering & Catalysis. 2018; 14(1):60-8, DOI: 10.9767/bcrec.14.1.2594.60-68

Ayuningtias DDR, Nurahmanto D, Rosyidi VA. Optimasi Komposisi Polietilen Glikol dan Lesitin sebagai Kombinasi Surfaktan pada Sediaan Nanoemulsi Kafein. Pustaka Kesehatan. 2017; 5(1): 157-63.

Zulfa E, Novianto D, Setiawan D. Formulasi Nanoemulsi Natrium Diklofenak Dengan Variasi Kombinasi Tween 80 dan Span 80: Kajian Karakteristik Fisik Sediaan. Media Farmasi Indonesia. 2019;14(1):1471-7.

Pareta DN. Formulasi Self Nanoemulsifying Drug Delivery System (SNEDDS) Minyak Atsiri Biji Pala (Mysristica fragrans Houtt). Tesis. 2016; Universitas Setia Budi, Surakarta.

Zhang D, Jiang J, Shi H, Lu L, Zhang M, Lin J, Lü T, Huang J, Zhong Z, Zhao H. Nonionic surfactant Tween 80-facilitated bacterial transport in porous media: A nonmonotonic concentration-dependent performance, mechanism, and machine learning prediction. Environmental Research. 2024; 251(2). https://doi. org/10.1016/j.envres.2024.118670

Pratiwi L, A.Fudholi A, R.Martien R, Pramono S. Physical and Chemical Stability Test of SNEDDS (Self-nanoemulsifying Drug Delivery. System) and Nanoemulsion Ethyl Acetate Fraction of Garcinia mangostana (2018).23(2): 84-90. doi.org/10.22146/ mot.28533

Chong Y, Yu D, Han R, Li Y, Gu Y, Lu Z, Nie F, Wang L, Cui H. Preparation of Luvangetin Nanoemulsions: Antimicrobial Mechanism and Role in Infected Wound Healing. Int J Nanomedicine. 2024; 19(June):54935509. DOI: 10.2147/IJN.S457322

Mannuela N, W Taurina W & R.Sari R, Preparasi Dan Evaluasi Nanopartikel Azitromisin Kitosan Dan Uji Aktivitas Antibakteri Terhadap Bakteri Propionibacterium Acnes. Doctoral dissertation, Tanjungpura University. Majalah Obat Tradisional (Traditional Medicine Journal). 2016;23(2): 84-90.

DOI: https://doi.org/10.24198/ijpst.v12i2.49866