The Development of Nano-mist Spray Propolis as Antioxidant

Ine Suharyani Suryaman; Lela Sulastri; Yayan Rizikiyan; Nasrul Wathoni; Cecep Suhandi; Lusy Noviani; Yuni Rahmasari

doi:10.24198/ijpst.v12i2.52433

The Development of Nano-mist Spray Propolis as Antioxidant

Ine Suharyani Suryaman, Lela Sulastri, Yayan Rizikiyan, Nasrul Wathoni, Cecep Suhandi, Lusy Noviani, Yuni Rahmasari

Abstrak

Propolis is a lipid-based compound from honeybees composed of plant wax, resin, and pollen. Due to its poor water solubility, its application is limited to lipid-based formulations with low bioavailability. This study aimed to develop a water-based propolis nano-mist spray with improved dispersion and evaluate its antioxidant activity. The nano-mist formulation was prepared using soy lecithin and Tween 80 in a phosphate buffer base. Evaluations included organoleptic characteristics, pH, particle size, zeta potential, and antioxidant activity by DPPH method. The propolis extract contained flavonoids, alkaloids, and tannins. The resulting spray showed a light brown color, a pH of 6.87 ± 0.03, particle size of 272.13 ± 4.25 nm, and zeta potential of -0.433 ± 0.35 mV. Antioxidant testing revealed moderate activity with an IC₅₀ value of 83.63 ± 11.88 ppm. This study supports the potential of nano-mist spray as a promising delivery system for propolis with antioxidant properties suitable for cosmetic and pharmaceutical applications.

Kata Kunci

Nano-mist spray, propolis, ukuran partikel, antioksidan, DPPH

Teks Lengkap:

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Referensi

Elnakady YA, Rushdi AI, Franke R, Abutaha N, Ebaid H, Baabbad M, et al. Characteristics, chemical compositions and biological activities of propolis from Al-Bahah, Saudi Arabia. Sci Reports 2017 71. 2017 Feb;7(1):1–13.

Bouchelaghem S. Propolis characterization and antimicrobial activities against Staphylococcus aureus and Candida albicans: A review. Saudi J Biol Sci. 2022;29(4):1936–46.

Silva FRG, Matias TMS, Souza LIO, Matos-Rocha TJ, Fonseca SA, Mousinho KC, et al. Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas. Brazilian J Biol. 2019;79(3):452–9.

Rosana M, Ahwan A, Qonitah F. Uji Kualitatif Aktivitas Antioksidan Ekstrak Etanol Propolis. Lumbung Farm J Ilmu Kefarmasian. 2021;2(2):154.

Erwin, Thamrin A, Syafrizal. Uji Fitokimia, Toksisitas Serta Antioksidan Ekstrak Propolis Pembungkus Madu Lebah Trigona Incisa Dengan Metode 2,2-diphenyl-1-picrylhidrazyl (DPPH). J Kim Mulawarman. 2016;14(1):54–60.

Martinotti S, Ranzato E. Propolis: A new frontier for wound healing?. Burns Trauma. 2015;3(1):1–7.

Wagh VD. Propolis: A wonder bees product and its pharmacological potentials. Adv Pharmacol Sci. 2013;2013.

Suharyani I, Pamudji JS, Wikarsa S, Kurniati NF. Natamycin Nanosuspension For Ophthalmic Drug Delivery System. Res J Pharm , Biol Chem Sci. 2017;8:113–20.

Puglia C, Lauro MR, Offerta A, Crascì L, Micicchè L, Panico AM, et al. Nanostructured Lipid Carriers (NLC) as Vehicles for Topical Administration of Sesamol: In Vitro Percutaneous Absorption Study and Evaluation of Antioxidant Activity. Planta Med. 2017;83(5):398–404.

Weng J, Tong HH., Chow F. In vitro release study of the polymeric drug nanoparticles: Development and validation of a novel method. Pharmaceutics. 2020;12(8):1–18.

Rahayu A, Ayu Cahya Rosyida D, Nuraini I, Buana Surabaya Jalan Dukuh Menanggal XII A, Kebidanan P, Sains dan Kesehatan Universitas PGRI Adi Buana Surabaya Jalan Dukuh Menanggal XII F. Formulasi dan Optimasi Nanostructured Lipid Carriers (NLC) Ketokonazol Menggunakan Full Factorial Design Formulation and Optimization of Nanostructured Lipid Carriers (Nlc) Ketoconazole Using Full Factorial Design. Med Sains J Ilm Kefarmasian. 2022;7(3):561–70.

Suhandi C, Wilar G, Lesmana R, Zulhendri F, Suharyani I, Hasan N, et al. Propolis-Based Nanostructured Lipid Carriers for α-Mangostin Delivery: Formulation, Characterization, and In Vitro Antioxidant Activity Evaluation. Molecules. 2023;28(16).

Soleimanian Y, Goli SAH, Varshosaz J, Sahafi SM. Formulation and characterization of novel nanostructured lipid carriers made from beeswax, propolis wax and pomegranate seed oil [Internet]. Vol. 244, Food Chemistry. 2018. 83–92 p. Available from: https://doi.org/10.1016/j.foodchem.2017.10.010

Elkhateeb OM, Badawy MEI, Noreldin AE, Abou-Ahmed HM, El-Kammar MH, Elkhenany HA. Comparative evaluation of propolis nanostructured lipid carriers and its crude extract for antioxidants, antimicrobial activity, and skin regeneration potential. BMC Complement Med Ther. 2022;22(1):1–13. https://doi.org/10.1186/s12906-022-03737-4

Depkes RI. Farmakope Indonesia edisi IV. Departemen Kesehatan Republik Indonesia. 1995.

Iswandana R, Sihombing LKM. Formulasi , Uji Stabilitas Fisik , dan Uji Aktivitas Secara In Vitro Sediaan Spray Antibau Kaki yang Mengandung Ekstrak Etanol Daun Sirih (Piper betle L.). Pharmaceutical Sciences and Research. 2017;4(3):121–31.

Wathoni N, Sari D., Suharyani I, Motoyama K, Mohammed AF., Cahyanto A, et al. Enhancement of α-Mangostin Wound Healing Ability by Complexation with 2-Hydroxypropyl-β-Cyclodextrin in Hydrogel Formulation. Pharmaceuticals. 2020;13(10):290.

Maulina L, Sugihartini N. Formulasi Gel ekstrak Etanol Kulit Buah Manggis (Garcinia mangostana L.) dengan Variasi Gelling Agent sebagai Sediaan Luka Bakar. Pharmaciana. 2015;5(1):43–52.

Ong TH, Chitra E, Ramamurthy S, Ling CCS, Ambu SP, Davamani F. Cationic chitosan-propolis nanoparticles alter the zeta potential of S. epidermidis, inhibit biofilm formation by modulating gene expression and exhibit synergism with antibiotics. PLoS One. 2019;14(2).

Alfira A. Uji aktivitas antioksidan ekstrak dan fraksi kulit batang sintok (Cinnamomum sintoc Blume). UIN Syarif Hidayatullah Jakarta; 2014.

Falya Y, Rizikiyan Y, Suharyani I, Amelia R, Yulia Senja R. Formulation and Evaluation of Lipstick with Braziline Pigment of Caesalpinia sappan L. J Farm Etam. 2022;1(2019):92–107.

Suharyani I, Falya Y, Rindiyani, Nurmaya N, Afidah Y. Pengaruh Pelarut Polar terhadap Aktivitas Antioksidan Daging Buah Naga Merah (Hylocereus polyrhizus) yang diekstraksi dengan Metode Microwave Assisted Extraction (MAE). Pharmacoscript. 2022;5(2):237–48.

Handayani V, Roskiana Ahmad A, Sudir M. Uji Aktivitas Antioksidan Ekstrak Metanol Bunga dan Daun Patikala (Etlingera elatior (Jack) R.M.Sm) Menggunakan Metode DPPH. Pharm Sci Res. 2014;1(2):86–93.

Satrialdi, Anjani Putri P, Yunus Aji Lumintang. Pengembangan Formula Nanostructured Lipid Carrier (NLC) Sebagai Pembawa Minyak Atsiri Melati (Jasminum officinale L.) serta Potensi Aktivitas Antioksidan. Maj Farm dan Farmakol. 2023;27(2):32–8.

Prabhakar K, Afzal SM, Surender G. Tween 80 containing lipid nanoemulsions for delivery of indinavir to brain. Acta Pharm Sin B [Internet]. 2013;3(5):345–53. Available from: http://dx.doi.org/10.1016/j.apsb.2013.08.001

Juliantoni Y, Hajrin W, Subaidah WA. Nanoparticle Formula Optimization of Juwet Seeds Extract (Syzygium cumini) using Simplex Lattice Design Method. J Biol Trop. 2020;20(3):416–22.

Ramadhani N. Formulasi dan Evaluasi Gel Structured Lipid Carriers (NLC) Adapalen. Universitas Bhakti Kencana; 2019.

Maharani I, Wigati S, Utami DT. Formulasi Nanopartikel Ekstrak Buah Naga (Hylocereus polyrhizus) sebagai Zat Warna Sediaan Lipstik. Chempublish Journal. 2017; 2(1):38-43.

Shahab-Navaei F, Asoodeh A. Synthesis of optimized propolis solid lipid nanoparticles with desirable antimicrobial, antioxidant, and anti-cancer properties. Sci Rep. 2023;13(1):1–14. https://doi.org/10.1038/s41598-023-45768-y

Damanis FVM, Wewengkang DS, Antasionasti I. Uji Aktivitas Antioksidan Ekstrak Etanol Ascidian Herdmania Momus dengan Metode DPPH (1,1-difenil-2-pikrilhidrazil). Pharmacon. 2020;9(3):464.

Wardaniati I, Yanti R. Uji Aktivitas Antioksidan Ekstrak Etanol Propolis Lebah Trigona (Trigona itama) Menggunakan Metode DPPH. Vol. 2, JOPS. 2018.

DOI: https://doi.org/10.24198/ijpst.v12i2.52433