Anti-Inflammatory Activity of Dysmenorrhea Herbal Medicine Through Inhibition of the Cyclooxygenase Enzyme and Identification of Compound in Sambiloto Extract

Nurul Muhlisa Mus; Fajar Prasetya; Lucie Widowati; Mentarry Bafadal; Nur Rezky Khairun Nisaa; Ani Isnawati

doi:10.24198/ijpst.v6i1.53219

Anti-Inflammatory Activity of Dysmenorrhea Herbal Medicine Through Inhibition of the Cyclooxygenase Enzyme and Identification of Compound in Sambiloto Extract

Nurul Muhlisa Mus, Fajar Prasetya, Lucie Widowati, Mentarry Bafadal, Nur Rezky Khairun Nisaa, Ani Isnawati

Abstrak

Dismenore adalah rasa kram atau nyeri yang terjadi ketika menstruasi. Meskipun efektif dalam pengobatan dismenore, penggunaan AINS jangka panjang dapat menyebabkan efek samping pada sistem gastrointestinal dan terjadi pendarahan. Tujuan penelitian ini adalah untuk mengetahui aktivitas antiinflamasi ramuan jamu dismenore yang terdiri dari ekstrak sambiloto, kunyit, kelor, jahe merah,dan coklat hitam terhadap enzim COX yang ditentukan dengan menggunakan metode TMPD secara spektrofotometri. Berdasarkan hasil pengujian, diketahui ekstrak sambiloto memiliki aktivitas penghambatan baik pada enzim COX-1 maupun enzim COX-2 dengan persen inhibisi masing-masing sebesar 4.5% (1000 ppm) dan 74.29% (1000 ppm). Berdasarkan hasil analisis metabolomik menggunakan LC-HRMS, salah satu senyawa yang terdeteksi pada sampel ekstrak etanol sambiloto yaitu senyawa turunan andrographolide yaitu 14-Deoxy-11,12-didehydro andrographolide dengan rumus kimia C₂₀H₂₈O₄. Senyawa paling melimpah kedua pada ekstrak sambiloto ini terdeteksi oleh ESI positif dengan waktu retensi 9.804 menit dan dengan berat molekul 332,1974.

Kata Kunci

Dismenorea;COX;sambiloto;kunyi;kelor;jahe merah;coklat hitam

Teks Lengkap:

PDF

Referensi

Rainone, F. Chapter 56 - Dysmenorrhea, Integrative Medicine (Second Edition), W.B. Saunders; 2007.

Nagy H, Carlson K, Khan MAB. Dysmenorrhea. [Updated 2023 Nov 12]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023.

Chauhan M, Kala J. Relation between dysmenorrhea and body mass index in adolescents with rural versus urban variation. J Obstet Gynaecol India.2012;62(4):442-5.

Alsaleem MA. Dysmenorrhea, associated symptoms, and management among students at King Khalid University, Saudi Arabia: An exploratory study. J Family Med Prim Care. 2018 Jul-Aug;7(4):769-774.

Proctor M, Farquhar C. Diagnosis and management of dysmenorrhoea. BMJ. 2006;332:1134–8.

Calis KA, Dang DK, Kalantaridou SN, Erogul M. Dysmenorrhea: practice essentials, background, pathophysiology. New York (NY): Medscape; 2019.

Evans, J.; Salamonsen, L.A. Inflammation, leukocytes and menstruation. Rev. Endocr. Metab. Disord. 2012;13:277–288.

Oladosu FA, Tu FF, Hellman KM. Nonsteroidal antiinflammatory drug resistance in dysmenorrhea: epidemiology, causes, and treatment. Am J Obstet Gynecol. 2018 Apr;218(4):390-400.

Doty E, Attaran M. Managing primary dysmenorrhea. J Pediatr Adolesc Gynecol. 2006 Oct;19(5):341-4

Bahrami, Afsane & Ariakia, Fatemeh & A.Ferns, Gordon & Ghayour-Mobarhan, Majid. The prevalence of menstrual problems amongst adolescent girls in northeastern Iran. Journal of Advances in Medical and Biomedical Research.2022; 30:1-8

Tabari, Naghmeh & Kheirkhah, Masoomeh & Mojab, Faraz & Salehi, Masoud. An Investigation of the Effect of Curcumin (Turmeric) Capsule on the Severity and Duration of Dysmenorrhea in Students of Iran University of Medical Sciences. Journal of Evolution of Medical and Dental Sciences.2020; 9:3444-3451.

khayat S, kheirkhah M, Fanaei H, Behboodi Moghadam Z, pourmohsen M, Kasaeiyan A. Comparison the effects of Ginger and Curcumin in treatment of premenstrual syndrome. Iran South Med J. 2015;18(3):575-586

Hwang, Y. H., Kim, T., Kim, R., & Ha, H. The Natural Product 6-Gingerol Inhibits Inflammation-Associated Osteoclast Differentiation via Reduction of Prostaglandin E₂ Levels. International journal of molecular sciences. 2018;19(7):2068.

Shimoda H, Shan SJ, Tanaka J, Seki A, Seo JW, Kasajima N, Tamura S, Ke Y, Murakami N. Anti-inflammatory properties of red ginger (Zingiber officinale var. Rubra) extract and suppression of nitric oxide production by its constituents. J Med Food. 2010 Feb;13(1):156-62

Tan W.S.D., Liao W., Zhou S., Wong W.S.F. Is there a future for andrographolide to be an anti-inflammatory drug? Deciphering its major mechanisms of action. Biochem. Pharmacol. 2017;139:71–81.

Coppin J.P., Xu Y., Chen H., Pan M.H., Ho C.T., Juliani R., Simon J.E., Wu Q. Determination of flavonoids by LC/MS and anti-inflammatory activity in Moringa oleifera. J. Funct. Foods. 2013;5:1892–1899.

Xu, Y. B., Chen, G. L., & Guo, M. Q. Antioxidant and Anti-Inflammatory Activities of the Crude Extracts of Moringa oleifera from Kenya and Their Correlations with Flavonoids. Antioxidants (Basel, Switzerland).2019; 8(8):296.

Fard, M. T., Arulselvan, P., Karthivashan, G., Adam, S. K., & Fakurazi, S. Bioactive Extract from Moringa oleifera Inhibits the Pro-inflammatory Mediators in Lipopolysaccharide Stimulated Macrophages. Pharmacognosy magazine. 2015;11(Suppl 4):S556–S563.

Pérez-Cano FJ, Massot-Cladera M, Franch A, Castellote C, Castell M. The effects of cocoa on the immune system. Front Pharmacol. 2013;4:71.

Borghi SM, Pinho-Ribeiro FA, Fattori V, Bussmann AJ, Vignoli JA, Camilios-Neto D, Casagrande R, Verri WA., Jr Quercetin inhibits peripheral and spinal cord nociceptive mechanisms to reduce intense acute swimming-induced muscle pain in mice. PLoS One. 2016;11(9):e0162267.

Liu J, Seibold SA, Rieke CJ, Song I, Cukier RI, Smith WL. Prostaglandin endoperoxide H synthases: peroxidase hydroperoxide specificity and cyclooxygenase activation. J Biol Chem. 2007 Jun 22;282(25):18233-18244.

Windarsih A, Suratno, Warmiko HD, Indrianingsih AW, Rohman A, Ulumuddin YI. Untargeted metabolomics and proteomics approach using liquid chromatography-Orbitrap high resolution mass spectrometry to detect pork adulteration in Pangasius hypopthalmus meat. Food Chem. 2022 Aug 30;386:132856.

Li, X., Yuan, W., Wu, J., Zhen, J., Sun, Q., & Yu, M. Andrographolide, a natural anti-inflammatory agent: An Update. Frontiers in pharmacology. 2022;13:920435.

Lim J. C., Goh F. Y., Sagineedu S. R., Yong A. C., Sidik S. M., Lajis N. H., et al. A semisynthetic diterpenoid lactone inhibits NF-κB signalling to ameliorate inflammation and airway hyperresponsiveness in a mouse asthma model. Toxicol. Appl. Pharmacol. 2016;302:10–22.

Liu Y. T., Chen H. W., Lii C. K., Jhuang J. H., Huang C. S., Li M. L., et al. A diterpenoid, 14-deoxy-11, 12-didehydroandrographolide, in andrographis paniculata reduces steatohepatitis and liver injury in mice fed a high-fat and high-cholesterol diet. Nutrients. 2020;12 (2): E523.

Gupta S., Mishra K. P., Kumar B., Singh S. B., Ganju L. Andrographolide attenuates complete freund's adjuvant induced arthritis via suppression of inflammatory mediators and pro-inflammatory cytokines. J. Ethnopharmacol. 2020; 261: 113022.

(diunduh 24 Januari 2024). Tersedia dari: https://www.chemspider.com/Chemical-Structure.4647111.html?rid=0b3dcaa3-e648-4775-b3b8-377621bb418e

DOI: https://doi.org/10.24198/ijpst.v6i1.53219