Antioxidant and Antibacterial Activity of Extract Combination from Sonneratia alba and Heterotrigona itama Propolis

Andini Nur Amilia; Paula Mariana Kustiawan

doi:10.24198/ijpst.v6i3.53776

Antioxidant and Antibacterial Activity of Extract Combination from Sonneratia alba and Heterotrigona itama Propolis

Andini Nur Amilia, Paula Mariana Kustiawan

Abstrak

Sonneratia alba or rambai leaves are a plant from East Kalimantan that the community has used for generations as a cold powder. Antioxidants are needed to ward off free radicals, while bacteria are one of the causes of acne. Propolis also shows potential as an antioxidant and antibacterial agent. This research evaluated whether the combination of Sonneratia alba leaves extract and Heterotrigona itama propolis can increase antioxidant and antibacterial activity and whether this combination has synergistic potential. The S. alba leaves and H. itama propolis were macerated using ethanol as the solvent. The antioxidant activity test using the DPPH method with three comparisons of 1:1, 1:2, and 2:1 produced IC50 values of 12.669 µg/ml, 32.756 µg/ml, and 19.694 µg/ml, respectively. The IC50 value for ascorbic acid is 12.293 µg/ml. The results show that the combination of S. alba leaves and H. itama propolis ethanol extract has very strong antioxidant activity. The antibacterial activity test against Staphylococcus aureus bacteria with three concentrations of 60%, 80%, and 100% showed inhibition zones of 10.5 mm (medium category), 16 mm, and 17.5 mm (strong category), respectively.

Kata Kunci

Antibacterial; Antioxidant; Heterotrigona itama; Propolis; Sonneratia alba.

Teks Lengkap:

PDF (English)

Referensi

Haerani A, Chaerunisa AY, Subarnas A. Antioksidan Untuk Kulit. Farmaka. 2018;16(2):135–51.

Irtanto O, Pangkahila A, Aman I. Pemberian Ekstrak Floret Pisang Raja (Musa x paradisiaca) Mencegah Penurunan Kadar Superoksida Dismutase (SOD) pada Hati Mencit (Mus musculus) BALB/c dengan Aktivitas Fisik Berlebih. Jurnal Biomedik. 2017;9(3):166–71.

Sukertiasih NK, Megawati F, Meriyani H, Sanjaya DA. Studi Retrospektif Gambaran Resistensi Bakteri terhadap Antibiotik. Jurnal Ilmiah Medicamento. 2021;7(2):108–11.

Manuhuttu D, Nur A, Saimima. Potensi Ekstrak Daun Mangrove (Sonneratia alba) Sebagai Antibakteri terhadap Salmonella, Staphylococcus aureus, dan Escherichia coli. BIOPENDIX: Jurnal Biologi, Pendidikan Dan Terapan. 2021;7(2):71–9.

Kustiawan PM, Zulfa AF, Batistuta MA, Hanifa DNC, Setiawan IM. Comparative Analysis of Phytochemical, Total Phenolic Content, Antioxidant and Antibacterial Activity of Two Species Stingless Bee Propolis from East Kalimantan. Malaysian Journal of Medicine and Health Science. 2022;18(7):50–5.

Hermalinda R, Taufiqurrahman I, Helmi ZN. Total Flavonoid Content Analysis Flavonoid Content Analysis of Ramania Leaves’ Extract using Ethanol, Methanol and N-hexane as Solvents. Dentino: Jurnal Kedokteran Gigi. 2019;4(1):60–3.

Senduk TW, Montolalu LADY, Dotulong V. Rendemen Ekstrak Air Rebusan Daun Tua Mangrove Sonneratia alba (The rendement of boiled water extract of mature leaves of mangrove Sonneratia alba). Jurnal Perikanan dan Kelautan Tropis. 2020;11(1):9.

Wicaksono IB, Ulfah M. Uji Aktivitas Antioksidan Kombinasi Ekstrak Etanol Daun Sirsak (Annona muricata L.) dan Daun Jambu Biji (Psidium guajava L.) dengan Metode DPPH (2,2-difenil-1-pikrihidrazil). Jurnal Inovasi Teknik Kimia. 2017;2(1):44–8.

Douw DD, Wardani TS. Uji Aktivitas Antioksidan Kombinasi Ekstrak Etanol Daun Alpukat (Persea americana Mill.) dan Daun Jambu Biji (Psidium guajava L.) Metode DPPH dan FRAP. Medfarm: Jurnal Farmasi dan Kesehatan. 2023;12(1):93–104.

Azzahra N, Hardiyanti T, Purnama, Zulfiawan. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Rumbai (Baccaurea dulkis Muell.Arg) Terhadap Bakteri (Staphylococcus aureus). Jurnal Kesehatan Terapan. 2022;9.

Nurhayati LS, Yahdiyani N, Hidayatulloh A. Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt dengan Metode Difusi Sumuran dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan. 2020;1(2):41.

Lestari G, Noptahariza R, Rahmadina N, Farmasi A, Bengkulu AF. Uji Aktivitas Antibakteri Formulasi Sabun Cair Ekstrak Kulit Buah Durian (Durio Zibethinus L.) Terhadap Bakteri Staphylococcus Aureus. Cendekia Journal of Pharmacy. 2020;4(2):95–101.

Nafis A, Septiani D, Malau J. Uji Aktivitas Antibakteri Ekstrak Daun Ceremai (Phyllanthus acidus (L.) Skeels) terhadap Staphylococcus aureus. Journal of Pharmaceutical and Sciences. 2023;6(3):1194–203.

Rastina, Sudarwanto M, Wientarsih I. Aktivitas Antibakteri Ekstrak Etanol Daun Kari Terhadap Staphylococcus aureus, Escherichia coli, dan Pseudomonas sp. Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences. 2015;9(2):185–8.

Sinaga FA. Stress Oksidatif dan Status Antioksidan pada Aktivitas Fisik Maksimal. Generasi Kampus. 2016;9(2):176–89.

Maharani, Riskierdi F, Febriani I, Kurnia KA, Rahman NA, Ilahi NF, et al. Peran Antioksidan Alami Berbahan Dasar Pangan Lokal dalam Mencegah Efek Radikal Bebas. Prosiding Seminar Nasional Biologi. 2021;1(2):390–9.

Rakhmawati IAI. Aktivitas Antioksidan DPPH dari Rumput Laut dengan Kajian Meta-Analisis. Tesis IPB. 2023.

Juliantri, Mariati NW, Rumondor J. Antibacterial Effectiveness Test of Water Hyacinth Leaf Extract (Eichhornia Crassipes) Against the Growth of Porphyromonas gingivalis Bacteria. Pharmacon. 2023;12(3):302–10.

Zeniusa P, Ramadhian MR, Nasution SH, Karima N. Uji Daya Hambat Ekstrak Etanol Teh Hijau terhadap Escherichia coli Secara In Vitro. Majority. 2019;8(2):136–43.

Kustiawan PM, Aziz A, Yuliawan VN. Antioxidant and Antibacterial Activity of Various Fractions of Heterotrigona itama Propolis Found in Kutai Kartanegara. Open Access Macedonia Journal of Medical Sciences. 2022;10(A):531–4.

Kustiawan PM, Yanti EN, Nisa K, Zulfa AF, Batistuta MA. Bioactivity of Heterotrigona itama propolis as anti-inflammatory: A review. Biointerface Research in Applcation Chemistry. 2023;13(4):326.

DOI: https://doi.org/10.24198/ijpst.v6i3.53776