Uniformity Test of Total Flavonoid Content in Antidiabetic Capsules from Ethanol Extract of Purple Sweet Potato Leaves (Ipomoea batatas L.)

Jessica Rianty Suhendi; Ni Kadek Santi Maha Dewi; Ni Kadek Warditiani; Cokorda Istri Sri Arisanti; I Made Agus Gelgel Wirasuta

doi:10.24198/ijpst.v12i2.53962

Uniformity Test of Total Flavonoid Content in Antidiabetic Capsules from Ethanol Extract of Purple Sweet Potato Leaves (Ipomoea batatas L.)

Jessica Rianty Suhendi, Ni Kadek Santi Maha Dewi, Ni Kadek Warditiani, Cokorda Istri Sri Arisanti, I Made Agus Gelgel Wirasuta

Abstrak

The ethanol extract of purple sweet potato leaves (Ipomoea batatas L. or “IBL”) has antidiabetic activity, and the total flavonoid (KTF) is designated as the quality marker (Q-marker) in capsule manufacturing. This research aims to develop KTF As a Q-marker for OHT capsules from ethanol extract of IBL leaves as antidiabetic. KTF Q-marker determination involves: (1) Identification of the absorption peak of the quercetin-AlCl3 complex, (2) Method verification, and (3) Testing OHT capsule content uniformity. The Quersetin-AlCl3 complex provides a bathochromic shift in band I from 380 nm before being reacted towards 430 nm after complex formation. The method verification test resulted in a correlation coefficient of 0.998, intraday RSD of 0.76-2.00%, and interday RSD of 0.87-1.96%, and accuracy of return in the range 82.88-91.83%. The consistency test results of the capsule content of the four bets obtained a ratio of the content of 10 capsules of each bet of 100% and RSD value in the range of 3.22-4.57% The establishment of KTF with a method in accordance with the FHI has met the verification requirements, showing that the method can be used and potentially as a quality control in the manufacture of IBL leaf capsules on a production scale.

Kata Kunci

Content Uniformity Test; Purple sweet potato leaves (Ipomoea batatas L.); Total Flavonoid Content

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Referensi

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DOI: https://doi.org/10.24198/ijpst.v12i2.53962