ANTI-INFLAMMATORY AND ANALGESIC ACTIVITY OF RETICULATED PYTHON FAT OIL (MALAYOPYTHON RETICULATUS) IN VIVO EVALUATION

Hajrah Hajrah; Dina Arindha Febriani; Niken Indriyanti

doi:10.24198/ijpst.v6i1.55201

ANTI-INFLAMMATORY AND ANALGESIC ACTIVITY OF RETICULATED PYTHON FAT OIL (MALAYOPYTHON RETICULATUS) IN VIVO EVALUATION

Hajrah Hajrah, Dina Arindha Febriani, Niken Indriyanti

Abstrak

Empirically, python fat oil (Malayopython reticulatus) is used by local people in Miau Baru, Kongbeng, East Kalimantan to treat swelling and inflammation. The purpose of this study was to determine the physical characteristics, anti-inflammatory, and analgesic activities of the python fat oil in reducing pain and inflammation. The methods that are used for determining physical characteristics are organoleptic test, density test, viscosity test, pH test, and refractive index test. The method that is used for determining anti-inflammatory activities is the paw edema method, which is induced by lambda carrageenan, and for analgesic activities using the hot plate method. The results obtained are that python oil has a yellow-orange color, a rancid odor, and has a bland taste, has a density of 0.870 g/mL with a pH of 4.1. The viscosity of the python fat oil is 2.2 Pa.s with a refractive index of 1.463. The percentage of edema formed at 5% concentration was 0.33%, 10% concentration was 0.43%, and 15% concentration was 0.25%. A recent increase in survival time in analgesic testing with a 5% concentration of 4.44%, a 10% concentration of 3.87%, and a 15% concentration of 3.11%. It was concluded that python fat oil has anti-inflammatory and analgesic activity.

Kata Kunci

Malayopython reticulatus; Reticulated python; Anti-Inflammatory; Analgesic; Python Fat Oil

Teks Lengkap:

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Referensi

Safitri, A. & Roosdiana, A. 2020. Biokimia Bahan Alam Analisis dan Fungsi. Malang: Media Nusa Creative.

Setyawati, M.. 2020. Electronical Games Untuk Mengatasi Nyeri Perawatan Luka Pada Anak Post Operasi. Yogyakarta: UNY PRESS.

Pariyanto, Santoso, Hidayat, T. & Darwin, C. 2021. Pemanfaatan Hewan Reptilia Sebagai Obat Tradisional Oleh Masyarakat Desa Taman Bogo Kecamatan Purbolinggo Kabupaten Lampung Timur. SETAWAR ABDIMAS, 1(1): 15–18.

Krisyanto, R.D., Ardian, H. & Anwari, M.S. 2019. Kajian Etnozoologi Untuk Pengobatan Suku Dayaksebaruk Di Desa Setunggul Kecamatan Silat Hilir Kabupaten Kapuas Hulu. Jurnal Hutan Lestari, 7(3): 1282–1289

Siswati, T., Sa’diyah, A., K., A.P., Rismaya, R., Sulistiana, D., Mardiyah, U., Kristanto, B., Anggraini, D.P., Indis, N. Al, Patimah, Aisyah, S., Sandra, L., Satriawan, D. & Rahmawati 2022. Kimia Analisis Bahan Pangan. Padang: PT. Global Eksekutif Teknologi.

Taufik, M., Ardilla, D., Tarigan, D.M., Thamrin, M., Razali, M. & Afritario, M.I. 2018. Studi Awal: Analisis Sifat Fisika Lemak Babi Hasil Ekstraksi Pada Produk Pangan Olahan. Agrintech: Jurnal Teknologi Pangan dan Hasil Pertanian, 1(2): 79–85.

Mukhtar, H.M., Kshirsagar, S.S. & Naseer, A. 2021. Pharmaceutical Organic Chemistry - I. Lucknow: Thakur Publication.

Manurung, L.S. & Sudrajad, H. 2018. Design and Build Up the Stirrer Viscometer. Jurnal Geliga Sains: Jurnal Pendidikan Fisika, 6(2): 98.

Silva, J.R., Burger, B., Kühl, C.M.C., Candreva, T., dos Anjos, M.B.P. & Rodrigues, H.G. 2018. Wound healing and omega-6 fatty acids: From inflammation to repair. Mediators of Inflammation, 2018.

Weimann, E., Silva, M.B.B., Murata, G.M., Bortolon, J.R., Dermargos, A., Curi, R. & Hatanaka, E. 2018. Topical anti-inflammatory activity of palmitoleic acid improves wound healing. PLoS ONE, 13(10): 1–10.

Saini, R.K., Assefa, A.D. & Keum, Y.S. 2021. Spices in the apiaceae family represent the healthiest fatty acid profile: A systematic comparison of 34 widely used spices and herbs. Foods, 10(4).

Pegoraro, N.S., Camponogara, C., Cruz, L. & Oliveira, S.M. 2021. Oleic acid exhibits an expressive anti-inflammatory effect in croton oil-induced irritant contact dermatitis without the occurrence of toxicological effects in mice. Journal of Ethnopharmacology, 267(April 2020).

Pereira, D.M., Correia-da-Silva, G., Valentão, P., Teixeira, N. & Andrade, P.B. 2014. Anti-inflammatory effect of unsaturated fatty acids and ergosta-7,22-dien-3-ol from Marthasterias glacialis: Prevention of CHOP-mediated ER-stress and NF-κB activation. PLoS ONE, 9(2).

Dermiati, T., Kamal, A., Tibe, F. & Anggi, V. 2018. Uji Antiinflamasi Ekstrak Etanol Kulit Batang Ceremai (Phyllanthus Acidus L.Skell) Terhadap Edema Kaki Tikus. Farmakologika Jurnal Farmasi, 15(1): 1–8.

Purnamasari, R. & Santi, D., R. 2017. Fisiologi Hewan. Surabaya: Program Studi Arsitektur UIN Sunan Ampel.

Octavianus, S., Fatimawali & Lolo, W.A. 2014. Uji Efek Analgetik Ekstrak Etanol Daun Pepaya (Carica Papaya L) Pada Mencit Putih Jantan (Mus Mucculus). Pharmacon, 3(2): 87–92.

Afandi, A. 2018. Anatomi dan Fisiologis Pada Tubuh Manusia. Ponorogo: Uwais Inspirasi Indonesia.

Mustaqin, H. 2023. Kajian Pemanfaatan Adsorben Ramah Lingkungan Pada Adorpsi Logam Berat Berbahaya Serta Optimasi. Banda Aceh: Syiah Kuala University Press.

DOI: https://doi.org/10.24198/ijpst.v6i1.55201