Evaluation of Sidr Leaf Ethanol (Ziziphus mauritiana Lam.) Extract-Based Tablets as Antihypercholesterolemia

Salmah Orbayinah; Yunita P. Dewi; Fariez Kurniawan; Annisa Krisridwany

doi:10.24198/ijpst.v12s1.57926

Evaluation of Sidr Leaf Ethanol (Ziziphus mauritiana Lam.) Extract-Based Tablets as Antihypercholesterolemia

Salmah Orbayinah, Yunita P. Dewi, Fariez Kurniawan, Annisa Krisridwany

Abstrak

Sidr leaf extract (Ziziphus mauritiana Lam.) is effective against hypercholesterolemia by blocking the HMG-CoA reductase. This study seeks to identify the characteristics of tablets formulated from the ethanol extract of Sidr leaf and their efficacy in lowering total blood cholesterol levels. The tablets were produced using the wet granulation method and processed into three formulations, F1, F2, and F3, with differing dosages of 100, 150, and 250 mg, along with placebo. The granules produced were evaluated for their physical characteristics. The tablets were evaluated for their physical characteristics and tested for effectiveness in lowering cholesterol levels. The efficacy of tablet-form sidr leaf ethanol extract was assessed by analyzing cholesterol levels in mouse serum samples compared to simvastatin. The data were statistically analyzed using one-way ANOVA and post-hoc Tukey HSD test. In the disintegration time test, F1 and F2 tablets satisfied the criteria. However, F3 exceeded the limit of 60 minutes. Among the three tablet doses, the F2 tablet (150 mg) demonstrated superior efficacy in lowering total cholesterol levels relative to the F1 and F3 tablets. Consequently, tablets containing Sidr leaf extract demonstrate potential for hypercholesterolemia management at an effective dosage of 150 mg, warranting further investigation through clinical trials.

Kata Kunci

cholesterol total; sidr leaf (Ziziphus mauritiana Lam.); tablet

Teks Lengkap:

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Referensi

Yani M. Mengendalikan kadar kolesterol pada hiperkolesterolemia. J Olahraga Prestasi. 2015;11(2):3–7.

Soran H, Adam S, Mohammad JB, Ho JH, Schofield JD, Kwok S, et al. Guidelines Hypercholesterolaemia – practical information for non-specialists. Arch Med Sci. 2018;14(1):1–21.

Malekmohammad K, Sewell RDE, Rafieian-Kopaei M. Antioxidants and atherosclerosis: Mechanistic aspects. Biomolecules. 2019;9(8):1–19.

El-Dahiyat F, Rashrash M, Abuhamdah S, Abu Farha R, Babar ZUD. Herbal medicines: A cross-sectional study to evaluate the prevalence and predictors of use among Jordanian adults. J Pharm Policy Pract. 2020;13(1):1–9.

WHO. WHO Traditional Medicine Strategy: 2014-2023. 2013.

Swarnkar SK. Effect of Aqueous Extract of Leaves of Ziziphus mauritiana on Few Parameters in High Fat Diet Induced Hyperlipidemia. 2018;

Abdulrahman MD, Zakariya AM, Hama HA, Hamad SW, Al-Rawi SS, Bradosty SW, et al. Ethnopharmacology, Biological Evaluation, and Chemical Composition of Ziziphus spina-christi (L.) Desf.: A Review. Adv Pharmacol Pharm Sci. 2022;2022:1–36.

Supari, Tangang F, Juneng L, Aldrian E. Observed changes in extreme temperature and precipitation over Indonesia. Int J Climatol. 2017;37(4):1979–97.

Yahia Y, Benabderrahim MA, Tlili N, Bagues M, Nagaz K. Bioactive compounds, antioxidant and antimicrobial activities of extracts from different plant parts of two Ziziphus Mill. species. PLoS One. 2020;15(5):1–16.

Jain P, Haque A, Islam T, Alam MA, Reza HM. Comparative evaluation of Ziziphus mauritiana leaf extracts for phenolic content, antioxidant and antibacterial activities. J Herbs, Spices Med Plants. 2019;25(3):236–58.

Youl ENH, Ouédraogo CAP, Gambo M, Ouédraogo M, Kiendrebéogo M, Traoré A, et al. Antioxidant activity of crude ethanolic extract and fractions of Ziziphus mauritiana Lam. (Rhamnaceae) leaves from Burkina Faso. J Basic Clin Physiol Pharmacol. 2019;30(3):1–9.

Olaiya CO, Omolekan TO. Antihypercholesterolemic activity of ethanolic extract of Buchholzia coriacea in rats. Afr Health Sci. 2013;13(4):1084–90.

Ashraf A, Sarfraz RA, Anwar F, Shahid SA, Alkharfy KM. Chemical composition and biological activities of leaves of ziziphus mauritiana l. Native to pakistan. Pakistan Journal of Botany. 2015;47(1):367–76.

Supratman S, Purwanti S, Rahardja DP.

Giving bidara leaf extract (Ziziphus mauritiana) through drinking water as an alternative antioxidant against quail haematological. In 2021.

Jain P, Haque A, Islam T, Alam MA, Reza HM. Comparative evaluation of Ziziphus mauritiana leaf extracts for phenolic content, antioxidant and antibacterial activities. Journal of Herbs, Spices and Medicinal Plants. 2019;25(3):236–58.

Karim MA, Hossain MK, Al-Mansur MA, Shajib MS, Rashid MA. Isolation of zizyberenalic acid and biological studies of Ziziphus mauritiana Lam. Growing in Bangladesh. Bangladesh Journal of Botany. 2019;48(1):163–8.

Bialangi N, Musa WJA, Situmeang B. Metabolite Profiling of Potential Fraction From Ethyl Acetate Extract of Ziziphus mauritiana Leaves by LC-MS/MS Analysis. Karbala International Journal of Modern Science. 2024;10(4):542–8.

Elhaty IA, Zeyoudi SA. A Comparative Study of the Phenolic and Flavonoids Contents, and Antioxidant Activity Of Ziziphus Mauritiana’s Leaves, Ripe and Unripe Fruit Extracts From UAE. Chemistry and Chemical Technology. 2024;18(3):363–71.

Aldhanhani ARH, Kaur N, Ahmed ZFR. Antioxidant phytochemicals and antibacterial activities of sidr (Ziziphus spp.) leaf extracts. Acta Horticulturae. 2022;1353:323–31.

Pauloi L, Salim RM, Chiang LK, Sundang M, Rusdi NA, Maid M, et al. GC-MS analysis of bioactive compounds in ethanolic extract on different parts of Ziziphus mauritiana. Biodiversitas. 2024;25(10):3442–53.

Kurniati NF, Garmana AN, Sakinah LN. The Effect of Ethanol Extract of Punica granatum Linn. Leaves on Lipid Profiles of Dyslipidemic Rat. Pharm Sci Res. 2021;8(2):86–92.

Hannan PA, Khan JA, Khan A, Safiullah S. Oral dispersible system: A new approach in drug delivery system. Indian J Pharm Sci. 2016;78(1):2–7.

Gaikwad SS, Kshirsagar SJ. Review on Tablet in Tablet techniques. Beni-Suef Univ J Basic Appl Sci. 2020;9(1):1–7.

Indonesia DKR. Farmakope Indonesia. IV. Jakarta: Departemen Kesehatan Republik Indonesia; 1995.

Lindani A. Perbandingan Pengukuran Kadar Air Metode Moisture Analyzer dengan Metode Oven pada Produk Biskuit Sandwich Cookies di PT Mondalez Indonesia Manufacturing. Departemen Ilmu dan Pangan Fakultas Teknologi Pertanian Institut Pertanian Bogor; 2016.

Siregar C. Proses Validasi Manufaktur Sediaan Tablet. Instituut Teknologi Bandung; 1992.

Indonesia DKR. Farmakope Indonesia. V. Jakarta: Departemen Kesehatan Republik Indonesia; 2014.

Kumar D, Singh J, Antil M K V. Quality Control of Tablets: A Review. Int J Univers Pharm Bio Sci. 2016;5(4):53–67.

Commission TBP. British Pharmacopoeia. London: The British Pharmacopoeia Commission; 2009.

Backer, A., and Van Den Brink B. Flora of Java (Spermatophytes Only). 1st ed. Groningen: The Netherlands, Noordhoff-; 1965.

Koirewoa, Y.A. F dan WIW. Isolasi dan Identifikasi Senyawa Flavonoid Dalam Daun Beluntas (Pluchea indica L.). 2012;

Prashant Tiwari, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur HK. Phytochemical screening and Extraction: A Review. Hepatology. 2017;66(6):1866–84.

BPOM. Peraturan badan pengawasan obat dan makanan nomor 32 tahun 2019 tentang persyaratan keamanan dan mutu obat tradisional. Vol. 11, Badan Pengawas Obat dan Makanan. 2019.

Putra DJS, Antari NW., Putri NPR., Arisanti CI., Samirana P. Penggunaan Polivinill Pirolidon (PVP) Sebagai Bahan Pengikat Pada Formulasi Tablet Ekstrak Daun Sirih (Piper betle L.). J Farm Udayana. 2019;8(1):14–21.

Syamsia, Pratiwi RD, Susana. Sifat Fisik Tablet Dihydroartemisinin-Piperaquin (Dhp) Sediaan Generik Dan Sediaan Dengan Nama Dagang Yang Beredar Di Kotamadya Jayapura. Pharmacon. 2017;6(3):310–4.

BPOM. Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Nomor 12 Tahun 2014 Tentang Persyaratan Mutu Obat Tradisional. Badan Pengawas Obat dan Makanan. 2014;1–25.

Smith KL. An improved test for uniformity of weight of tablets. J Pharm Pharmacol. 1955;7(1):875–81.

Soeratri W, Nuruddin MN, Paramita DP, Setyawan D, Widjaja B. Optimization of Povidone K-30 and Sodium Starch Glycolate on Levofloxacin Tablet by Factorial Design. J Ilmu Dasar. 2020;21(1):35–42.

Lindberg NO, Jönsson C, Holmquist B. Optimization of disintegration time and crushing strength of a tablet formulation. Drug Dev Ind Pharm. 1985;11(4):931–43.

Stevens H, Voelker M, Gow L, MacDougall F, Bieri G. In-vivo disintegration and absorption of two fast acting aspirin tablet formulations compared to ibuprofen tablets using pharmacoscintigraphy. J Drug Deliv Sci Technol. 2019;51(February):535–41.

Serra CH dos R, Chang KH, Dezani TM, Porta V, Storpirtis S. Dissolution efficiency and bioequivalence study using urine data from healthy volunteers: A comparison between two tablet formulations of cephalexin. Brazilian J Pharm Sci. 2015;51(2):383–92.

Khan KA. The concept of dissolution efficiency. J Pharm Pharmacol. 1975;27(1):48–9.

Shanmugam S. Granulation techniques and technologies: recent progresses. BioImpacts. 2015;5(1):55–63.

Gomes AF, Almeida MP, Ruela ALM, Amaral JG, David JM, Leite MF. Development and evaluation of physical and release properties of a tablet formulation containing dry hydroethanolic extract from Lippia alba leaves. J Herb Med. 2021;29.

Pandey H, Srivastava S, Mishra B, Saxena R, Tripathi YB. Development and evaluation of herbal tablet loaded with Pueraria tuberosa water extract with use of different excipients. Asian J Pharm. 2018;12(2):786–93.

Mudrić J, Arsenijević J, Maksimović Z, Ibrić S, Gopčević K, Đuriš J. Tablet and capsule formulations incorporating high doses of a dry optimized herbal extract: The case of Satureja kitaibelii. J Drug Deliv Sci Technol. 2021;66(March):102776.

Dahiru D, Obidoa O. Evaluation of the antioxidant effects of Ziziphus mauritiana lam. leaf extracts against chronic ethanol-induced hepatotoxicity in rat liver. African J Tradit Complement Altern Med. 2008;5(1):39–45.

DOI: https://doi.org/10.24198/ijpst.v12s1.57926