Indonesian Journal of Pharmaceutical Science and Technology
Pengaruh Konsentrasi Etanol Terhadap Aktivitas Antioksidan Seledri (Apium graveolens) secara In Vitro dan In Vivo
Abstrak
Reactive oxygen species (ROS) yang terbentuk akibat aktivitas fisik yang berlebih akan mengoksidasi LDL (low-density lipoprotein) dan memicu terbentuknya arteriosklerosis. Peningkatan konsumsi antioksidan terbukti dapat menghindari penyakit jantung, salah satunya bersumber dari bumbu dapur seperti seledri (Apium graveolens). Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi etanol terhadap aktivitas antioksidan herba seledri secara in vitro dan in vivo. Simplisia dimaserasi menggunakan campuran pelarut etanol dan air dengan berbagai konsentrasi (air 100%, etanol 50%, etanol 70%, etanol 90%, dan etanol 96%). Aktivitas antioksidan ditentukan menggunakan metode LDL oxidation dan β-carotene bleaching secara in vitro,sedangkan secara in vivo menggunakan hewan uji tikus metode high intensity swimming exercises. Hasil penelitian menunjukkan bahwa ekstrak etanol 70% secara signifikan (p<0,05) mampu menghambat lalu degradasi β-karoten dan LDL dibandingkan dengan ekstrak lainnya. Pada pengujian in vivo, ekstrak etanol 70% juga mampu menurunkan kadar malondialdehid (14,35±0,63 ng/mL) dibandingkan dengan kontrol negatif (46,04±1,69 ng/mL). Selain itu, ditemukan peningkatan kadar glutation setelah pemberian ekstrak etanol 70% (111,93±11,68 ng/mL) dibandingkan dengan kontrol negatif (46,04±1,69 ng/mL). Sehingga dapat disimpulkan bahwa ekstrak etanol 70% herba seledri berpotensi untuk dikembangkan sebagai obat pencegah arteriosklerosis.
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DOI: https://doi.org/10.24198/ijpst.v12i3.60387
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