Potential of avocado seed ethanol extract (Persea americana Mill. var Miki) as an anti-aging

Syadza Alya; Kartini Hasballah; Fitria Fitria; Essy Harnelly; Nirwana Lazuardi Sary

doi:10.24198/ijpst.v13i1.69540

Potential of avocado seed ethanol extract (Persea americana Mill. var Miki) as an anti-aging

Syadza Alya, Kartini Hasballah, Fitria Fitria, Essy Harnelly, Nirwana Lazuardi Sary

Abstrak

Skin aging, characterized by decreased skin elasticity, is a global concern closely related to elastin degradation caused by oxidative stress and elastase activity. Natural ingredients rich in antioxidants are increasingly explored as potential anti-aging agents. Avocado (Persea americana) seeds contain various bioactive compounds that may protect elastin and slow the skin aging process. This study employed in silico and in vitro approaches to evaluate the antioxidant and anti-elastase activities of ethanol extract of avocado seeds from Bener Meriah, Aceh. Extraction was performed using maceration with 70% ethanol, followed by phytochemical screening. Antioxidant activity was assessed using the DPPH method. Anti-elastase activity was evaluated in silico through molecular docking against porcine pancreatic elastase (PPE) and in vitro using the SANA substrate and PPE enzyme. The avocado seed ethanol extract showed strong antioxidant activity with an IC₅₀ value of 47.31 ppm. It also demonstrated potent anti-elastase activity with an IC₅₀ value of 6.980 ppm. Molecular docking revealed that cholestan-3-ol, 2-methylene-, (3β,5α)- had a binding affinity of −8.0 kcal/mol, comparable to the control. Overall, the ethanol extract of avocado seeds exhibits very strong antioxidant and significant anti-elastase activities both in silico and in vitro, indicating its potential as a natural anti-aging agent.

Kata Kunci

Anti-aging; in silico, in vitro, Persea Americana Mill; Porcine Pancreatic Elastase

Teks Lengkap:

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Referensi

Tu Y, Quan T. Oxidative stress and human skin connective tissue aging. Cosmetics. 2016;3:28.

Zhang X, Yang X, Liu X, Huang J, Zhang Y, Xu X, et al. Decoding skin aging: the role of KNG1 in collagen and elastic fibre degradation. Mech Ageing Dev. 2023;210:111776.

Kerns ML, Chien AL, Kang S. Skin aging. In: Kang S, Amagai M, Bruckner AL, et al., editors. Fitzpatrick’s Dermatology. 9th ed. New York (NY): McGraw-Hill Education; 2019.

Lee H, Hong Y, Kim M. Structural and functional changes and possible molecular mechanisms in aged skin. Int J Mol Sci. 2021;22(22):12489.

Reichheld SE, Muiznieks LD, Lu R, Sharpe S, Keeley FW. Sequence variants of human tropoelastin affecting assembly, structural characteristics and functional properties of polymeric elastin in health and disease. Matrix Biol. 2019;84:68–80.

Wei M, He X, Liu N, Deng H. Role of reactive oxygen species in ultraviolet-induced photodamage of the skin. Cell Div. 2024;19(1):1.

Petruk G, Di Giudice R, Rigano MM, Monti DM. Antioxidants from plants protect against skin photoaging. Oxid Med Cell Longev. 2018;2018:1454936.

Hossain A, Shahidi F. Persimmon leaves: nutritional, pharmaceutical, and industrial potential—A review. Plants. 2023;12(4):937. doi:10.3390/plants12040937.

Ifesan BOT, Olorunsola BO, Ifesan BT. Nutritional composition and acceptability of candy from avocado seed (Persea americana). Int J Adv Innov Res. 2015;3(6):1630–1634.

[Jurnal Tersedia] Nguyen, Van Lam & Nguyen, & Tran, Thi & Bui, Thi & Paxayavong, Souksavanh & Lan, Thi & Tran, Huong. (2022). Bioactive compounds, antioxidant activity and lipid content of various avocado fruits. Carpathian Journal of Food Science and Technology. 10.34302/crpjfst/2022.14.2.4.

da Vinha AF, Moreira JMR, Barreira S. Physicochemical parameters, phytochemical composition and antioxidant activity of the algarvian avocado (Persea americana Mill.). J Agric Sci. 2013;5:100.

Segovia FJ, Hidalgo GI, Villasante J, Ramis X, Almajano MP. Avocado seed: A comparative study of antioxidant content and capacity in protecting oil models from oxidation. Molecules. 2018;23(10):2421.

Bangar SP, Dunno K, Dhull SB, Siroha AK, Changan S, Maqsood S, Rusu AV. Avocado seed discoveries: Chemical composition, biological properties, and industrial food applications. Food Chem X. 2022;16:100507.

Działo M, Mierziak J, Korzun U, Preisner M, Szopa J, Kulma A. The potential of plant phenolics in prevention and therapy of skin disorders. Int J Mol Sci. 2016;17(2):160.

Riwanti P, Izazih F, Amaliyah A. Pengaruh perbedaan konsentrasi etanol pada kadar flavonoid total ekstrak etanol 50, 70, dan 96% Sargassum polycystum dari Madura. J Pharm Care Anwar Med. 2020;2(2):82–95.

More PR, Arya SS. Intensification of bio-actives extraction from pomegranate peel using pulsed ultrasound: Effect of factors, correlation, optimization and antioxidant bioactivities. Ultrason Sonochem. 2021;72:105423.

Sulistyarini I, Sari DA, Wicakson TA. Skrining fitokimia senyawa metabolit sekunder batang buah naga (Hylocereus polyrhizus). J Ilm Cendekia Eksakta. 2020;5(1):56–62.

Jubaidah S. Avocado seed extracts (Persea americana Mill.) bioactive compounds profile as a source of antioxidants. Indones J Pharm Sci Technol. 2024.

Azzahra F, Sari IS, Ashari DN. Penetapan nilai rendemen dan kandungan zat aktif ekstrak biji alpukat (Persea americana) berdasarkan perbedaan pelarut ekstraksi. J Farmasi Higea. 2022;14(2):159.

Ong ES, Low J, Tan JCW, Foo SY, Leo CH. Valorization of avocado seeds with antioxidant capacity using pressurized hot water extraction. Sci Rep. 2022;12(1):13036.

Kupnik K, Primožič M, Kokol V, Knez Ž, Leitgeb M. Enzymatic, antioxidant, and antimicrobial activities of bioactive compounds from avocado (Persea americana L.) seeds. Plants (Basel). 2023;12:1201.

Pientaweeratch S, Panapisal V, Tansirikongkol A. Antioxidant, anti-collagenase and anti-elastase activities of Phyllanthus emblica, Manilkara zapota and silymarin: an in vitro comparative study for anti-aging applications. Pharm Biol. 2016;54(9):1865–72.

Wittenauer J, Mäckle S, Sußmann D, Schweiggert-Weisz U, Carle R. Inhibitory effects of polyphenols from grape pomace extract on collagenase and elastase activity. Fitoterapia. 2015;101:179–87.

Pitasi G, Brancale A, Floris S, Fais A, Gitto R, De Luca L. Computational approach to identifying new chemical entities as elastase inhibitors with potential antiaging effects. Int J Mol Sci. 2024;25:11174.

Kumar N, Goel N. Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnol Rep. 2019;24:e00370.

Natanael GI, Simorangkir GF, Purba NP, Mardiana PBT, Amansyah A, Nasution AN. Potensi antioksidan dan anti-elastase ekstrak daun kelor (Moringa oleifera) terhadap antiaging. J Keperawatan Prior. 2021;4(1):69–76.

Desmiaty Y, Mulatsari E, Saputri FC, Hanafi M, Prastiwi R, Elya B. Inhibition of pancreatic elastase in silico and in vitro by Rubus rosifolius leaves extract and its constituents. J Pharm Bioallied Sci. 2020;12(3):1–7.

Álvarez-Martínez FJ, Herranz-López M, Barrajón-Catalán E, Micol V. Quantifying the modulation of elastase enzyme activity through colorimetric analysis. J Vis Exp. 2025.

Sholikha M. Penapisan in silico basis data senyawa tanaman obat di Indonesia dan uji in vitro aktivitas penghambatan elastase daun Melaleuca leucadendron L. [Thesis]. Depok (ID): Universitas Indonesia; 2013.

Swandiny GF, Desmiaty Y, Rahmah HU. Antioxidant and anti-aging activities from zodia (Evodia suaveolens J.R. Forst & G. Forst) fruit and leaf extract. J Jamu Indones. 2022;7(1):20–24

DOI: https://doi.org/10.24198/ijpst.v13i1.69540