Xylitol Production from Oil Palm Empty Fruit Bunches (OPEFB) Via Simultaneous Enzymatic Hydrolysis and Fermentation Process

Efri Mardawati, Nadya Maharani, Dwi Wahyudha Wira, Budi Mandra Harahap, Tri Yuliana, Een Sukarminah

Abstract

Oil palm empty fruit bunches (OPEFB) are the waste of palm oil processing and lignocellulosic biomass that can be used as raw material for xylitol production. In this research, bioconversion of xylitol using the Simultaneous Saccharification and Fermentation (SSF) process in one reactor with a short time and could save the cost. Pretreatment requires to degrade the lignin compound with thermal pretreatment. Hydrolysis of OPEFB performs enzymatically by commercial xylanase enzyme that is Cellic Htec2 and continued with fermentation by yeast Debaryomyces hansenii. The research used experimental and descriptive analysis that covers of variation of OPEFB substrate concentration (7.5%, 10%, 15%, 20%) on specific growth rate (µ), the yield of xylitol and substrate utilization. The results showed that an increase of OPEFB substrate concentration affected by the increase of specific growth rates  (μ) are 0.091/h, 0.094/h, 0.095/h and 0.126/h. It also affected to the decreasing of xylitol yield on 42 hours fermentation are 0.201 g/g; 0.189 g/g; 0.170 g/g; and 0.104 g/g.

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References

Ariffin, H.; Hassan, M.A.; Kalsom, M.S.U.; Abdullah, N.; Shirai, Y. Effect of physical, chemical and thermal pretreatments on the enzymatic hydrolysis of oil palm empty fruit bunch (OPEFB). J Trop Agric and Fd Sc 2008, 36, Malaysian Agricultural Research and Development Institute.

Bailey, M.J.; Biely P.; Pountanen, K. Inter-laboratory testing of methods for assay of xylanase activity. J Biotechnol 1992, 23, 257-270, https://doi.org/10.1016/0168-1656(92)90074-J.

Datta, R. Acidogenic fermentation of lignocellulose-acid yield and conversion of components. Biotechnol Bioeng 1981, 23, 2167-217. https://doi.org/10.1002/bit.260230921.

Faisal, A. Xylitol production from sugarcane bagasse by yeast producing xylose reductase (XR) enzyme. Bachelor Thesis. University of Indonesia, Depok, Indonesia, 2008.

Hamzah, N.H.C.; Markom, M.; Harun, S; Hassan, O. he Effect of Various Pretreatment Methods on Empty Fruit Bunch for Glucose Production. Malay J Anal Sci 2016, 20.

Harun, N. A. F.; Baharuddin, A. S.; Zainudin, M. H. M.; Bahrin, E. K.; Naim, M. N.; Zakaria, R. Cellulase Production from Treated Oil Palm Empty Fruit Bunch Degradation by Locally Isolated Thermobifida fusca. Bioresouces 2013, 8.

Mardawati, E., Kresnowati, MTAP., Purwadi, R., Bindar, Y., Setiadi, T. (2018). Fungal Production of Xylanase from Oil Palm Empty Fruit Bunch via Solid State Fermentation. International Journal on Advance Science, Engineering and Information Technology, 8(6), 2539-2547

Mardawati, E., Werner, A., Bley, T., Kresnowati, M., & Setiadi, T. The Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunches to Xylose. J Jpn Inst Energy 2014, 93: 973-978. https://doi.org/10. 3775/jie.93.973.

Mardawati, E., Wira, D. W., Kresnowati, M., Purwadi, R., & Setiadi, T. Microbial Production of Xylitol from Oil Palm Empty Fruit Bunches Hydrolysate : The Effect of Glucose Concentration. J Jpn Inst Energy 2015, 94, 769–774, https://doi.org/10.3775/jie.94.769.

Mardawati, E., Purwadi, R. Kresnowati, MTA., Setiadi, T. Evaluation of The Enzymatic Hydrolysis Process of Oil Palm Empty Fruit Bunch Using Crude Fungal Xylanase. ARPN Engineering and Applied Sciences, 2017, 12(18), 5286–5292.

Margono. Cell growth kinetics of Bacillus sp. In glucose medium as carbon source and ammonium sulphate as nitrogen source. Ekuilibrium 2010, 57-61.

Nascimento, D.C.O.; Ferreira, A.S.; Monteiro, S.N.; Aquino, R.C.M.P.; Kestur, S.G. Studies on The Characterization of Piassava Fibers and Their Epoxy Composites. Appl Sci Manuf 2012, 43, 353-362.

Ni’mah, L.; Ardiyanto, A; Zainuddin, M. Bioethanol production by yeast from oil palm fiber through method of pretreatment, acid hydrolysis, and fermentation. Info Teknik 2015, 16, 227-242.

Nobre, A.; Duarte, L.C.; Roseiro, J.C.; Girio, F.M. A physiological and enzymatic study of Debaryomyces hansenii growth on xylose and oxygen-limited chemostats. Appl Microbiol Biotechnol 2002, 59, 509-516, https://doi.org/10.1007/s00253-002-1050-4.

Novia, A.; Windarti; Rosmawati. Bioethanol production from rice straw with method of ozonolysis pretreatment-simultaneous saccharification and fermentation (SSF). Jurnal Teknik Kimia 2014, 20.

Omar, F.N.; Mohammed, M.A.P.; Baharuddin, A.S.; Microstructure modelling of Silica Bodies from Oil Palm Empty Fruit Bunch (OPEFB) Fibres. Bioresource 2014, 9, 938-951.

Parajó, J.C., Dominguez, H. & Dominguez, J.M., Biotechnological Production Of Xylitol Part I: Interest Of Xylitol And Fundamentals Of Its Biosynthesis. Bioresource Technology, 1998. 65, pp. 191–211.

Purnamayani, R. Technology of compost production from oil palm empty fruit bunches. Balai Pengkajian Teknologi Pertanian (BPTP) 2013, Jambi.

Saksono, B. 2006. Rare Sugars; unexplored gifts. Bio Trends 2006, 1, 35-37. Research center of biotechnology, Indonesian Institute of Science, Cibinong.

Wahyuni, A; Susilowati; Setyaningsih, R. Optimation xylitol production with variation of sugar cane bagasse hemicellulose hydrolysate concentration by Candida tropicalis. Biofarmasi 2004, 2, 29-34.

Winkelhausen, E.; Kuzmanova, S. Microbial Conversion of D-Xylose to Xylitol, J Fer and Bioeng 1998, 86.

DOI: https://doi.org/10.24198/jiita.v2i1.25064

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