Growth optimization of white turmeric (Curcuma zedoaria) plantlets with growth regulators gibberellins

Murgayanti Murgayanti, Fenny Dewi Nuroktavianti, Anne Nuraini

Abstract


 

The addition of cytokinin to the multiplication of white turmeric (Curcuma zedoaria) seedlings in vitro proved effective in shoot multiplication, but the addition of high cytokinin concentrations could cause stunted shoots and stunted growth. The addition of the hormone gibberellins (GA­3­) is often used in tissue culture for shoot elongation, so increasing the viability of plantlets. The experiment aims to determine the effect of GA3  and obtain the best concentrations of GA3 consisting of 0.50, 1, and 1.5 ppm GA3 on optimizing the growth of zedoary plantlets. The experimental parameters included the number of shoots, roots, leaves, plantlet height, and chlorophyll content observed at 6 WAP after subculturing. The results showed that giving 1 ppm GA3 had the best effect on optimizing plantlets' growth, namely the growth component of the number of shoots and plantlets' height.


Keywords


Curcuma zedoaria; Thidiazuron; Plantlets height.

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References


Asra R, Samarlina RA, Silalahi M. 2020. Hormon tumbuhan (I. Jatmoko, Ed.). UKIPress.http://repository.uki.ac.id/1579/

Bagale P, Pandey S, Regmi P, Bhusal S. . 2022. Role of plant growth regulator “Gibberellins” in vegetable production: an overview.International journal of horticultural science and technology, 9(3):291-299.

Behera S, Kamila PK, Rout KK, Barik DP, Panda PC, Naik SK. 2018. An efficient plant regeneration protocol of an industrially important plant, Hedychium coronarium J. koenig and establishment of genetic and biochemical fidelity of the regenerants. Industrial crops and product, 126: 58-68. Doi: 10.1016/j.indcrop.2018.09.058

Deepa AV, Anju M, Thomas TD. 2018. The applications of TDZ in medicinal plant tissue culture. Thidiazuron: from urea derivative to plant growth regulator. Doi: 10.1007/978-981-10-8004-3_15

Do Vale PAA, Junior JBDO, Costa FHDS, Pereira JES. 2019. Height and number of shoots on the survival and development of micropropagated bamboo plantlets during pre-acclimatization. Pesquisa agropecuaria, 49: 1-8. Doi: 10.1590/1983-40632019v4953751.

Farida, Rohaeni N. 2019. Pengaruh konsentrasi hormon giberelin terhadap pertumbuhan dan hasil tanaman okra. Ziraa’ah, 44(1): 1-8

Fong YM, Sani HB. 2019. Studies on micropropagation of Curcuma caesia Roxb. (Kunyit Hitam). IJISET, 6(4): 101-106.

Kajla S, Kala S, Kumar A, Mir H, Singh MK. 2018. Effect of growth regulator on in vitro shoot multiplication and plant regeneration of Rosa hybriad L. from nodal explants. Int. J. Curr. Microbiol. App. Sci., Special Issue-7:3804-3811.

Kholifah N, Shofiyani A, Purnawanto AM, Puspawiningtiyas E. 2022. Pengaruh konsentrasi NAA dan TDZ (Thidiazuron) terhadap organogenesis kalus kencur (Kaempferia galangal L.). Agritech, 24(2): 1411-1063.

Mastur. 2015. Sinkronisasi source and sink untuk peningkatan produktivitas biji pada tanama jarak pagar Buletin tanaman tembakau, serat & minyak industry, 7(1): 52-68.

Miceli A, Moncada A, Sabatino L, Vetrano F. 2019. Effect of gibberellic acid on growth, yield, and quality of leaf lettuce and rocket grown in a floating system. Agronomy, 9(7): 1-22. Doi: 10.3390/agronomy9070382

Noor F, Hossain F, Ara U. 2017. Effect of gibbrellic acid (GA3) on growth and yield parameters of french bean (Phaseolus vulgaris L.). Journal of the asiatic society of bangladesh science, 43(1): 49-60. Doi: 10.3329/jasbs.v43i1.46243

Novianto A, Setiawan AW. 2019. Pengaruh variasi sumber cahaya led terhadap pertumbuhan dan hasil tanaman selada (Lactuca sativa var. Crispa L) dengan sistem budidaya hidroponik rakit apung. Agric, 31(2).

Padron IES, Meza PMP, Diaz CML. 2020. Evaluation of sucrose and GA3 in an vitro shoot culture of Alpinia purpurata (Zingiberaceae). Scientific and technological research article, 21(2): 1-12. Doi: 10.21930/rcta.vol21_numn2_art:1993.

Puspita SD, Yulianti R, Mozartha M. 2019. The effectiveness of white turmeric (Curcuma zedoaria) extract as root canal irrigation alternative material on Streptococcus viridans. IOP Conf. Series: Journal of Physics: Conf. Series 1246. Doi:10.1088/17426596/1246/1/012040

Rahman N, Fitriani H, Rahman N, Hartati NS. 2021. Pengaruh beragam zat pengatur tumbuh terhadap induksi kalus organogenik dari ubi kayu (Manihot esculenta Crantz) genotype gajah dan kuning. Jurnal ilmu dasar, 22(2): 119-126.

Rahman SF, Mudyantini W, Anggarwulan E. 2019. Improving the quality and quantityof hemp fiber (Boehmeria nivea) by giving indole acetic acid and gibberellic acid. Cell biology and development, 3(1): 19-29. Doi: 10.13057/cellbioldev/v030104

Rajagukguk S, Dwiyani R, Astawa ING. 2018. Pengaruh konsentrasi GA3 terhadap induksi tunas tanaman anggur (Vitis vinivera L.) secara in vitro. Ejurnal agroekoteknologi tropika, 7(2): 285-294

Sagita ND, Sopyan I, Hadisaputri YE. 2022. Kunir putih (Curcuma zedoaria Rocs.): formulasi, kandungan kimia dan aktivitas biologi. Majalah farmasetika 7(3): 198-205.

Salim Z, Munadi E. 2017. Info Komoditi Tanaman Obat. Jakarta: Badan Pengkajian dan Pengembangan Perdagangan Kementrian Perdagangan Republik Indonesia.

Statistics Indonesia. 2021.Produksi Tanaman Biofarmaka.Diakses pada 08 Maret 2023, dari https://www.bps.go.id.

Triani N, Permatasari VP, Guniarti. 2020. Pengaruh konsentrasi dan frekuensi pemberian zat pengatur tumbuh giberelin (GA¬3¬) terhadap pertumbuhan dan hasil tanaman terung (Solanum melongena L. cv. Antaboga-1).Agro Bali:Agricultural journal, 3(2):144-155. Doi: 10.37637/ab.v3i2.575.

Wen Y, Shu-Chai S, Ma L-y, Wang X-n. 2018. Effects of gibberellic acid on photosynthesis and endogenous hormones of Camellia oleifera Abel. in 1st and 6th leaves. Journal of forest research, 23(5): 309–317. Doi: 10.1080/13416979.2018.1512394

Zhao B, Su Y. 2014. Process effect of microalgal-carbon dioxide fixation and biomass production: A review. renewable and sustainable energy, 31: 121-132. Doi: 10.1016/j.rser.2013.11.054

Ziraluo YPB. 2021. Metode perbanyakan tanaman ubi jalar ungu (Ipomea batatas poiret) dengan teknik kultur jaringan atau stek planlet. Jurnal inovasi Penelitian 2(3): 1037-1046.




DOI: https://doi.org/10.24198/kultivasi.v22i1.39163

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