Callus induction and proliferation of Centella asiatica L. generated from leaves and petioles in the presence of Dicamba and BAP

Suci Rahayu; Darmawan Saptadi; Chotimatul Azmi; Kusumawaty Kusumanegara; Tri Handayani; Ika Roostika; Nurliani Bermawie; Haris Maulana

doi:10.24198/kultivasi.v22i3.50581

Callus induction and proliferation of Centella asiatica L. generated from leaves and petioles in the presence of Dicamba and BAP

Suci Rahayu, Darmawan Saptadi, Chotimatul Azmi, Kusumawaty Kusumanegara, Tri Handayani, Ika Roostika, Nurliani Bermawie, Haris Maulana

Abstract

Centella's need for industrial raw materials is high as a medicinal plant. These needs can be met through rapid multiplication using tissue culture techniques. In this study, induction and proliferation for a callus of centella cv. Castina 3 was conducted in the MS basal medium plus 4 mgL^-1Dicamba withand enriched with 7 concentrations of BAP (0, 0.1, 0.3, 0.5, 0.7, 0.9, and 1.1 mgL^-1). Two kinds of explant were used, i.e., leaf and petiole. The results revealed that the addition of BAP in MS plus Dicamba medim stimulated better and produced a higher callus growth rate, both from leaf and petiole explants, than that media with Dicamba alone. Furthermore, 4 mgL^-1Dicamba + 1.1 mgL^-1BAP had a friable callus in the induction phase and a friable-compact callus in the proliferation phase. From this finding, it can be considered to use a combination of 4 mgL^-1Dicamba with 1.1 mgL^-1BAP in callus induction and proliferation for Centella rapid multiplication.

Keywords

Dicamba; BAP; callus induction; proliferation; Centella

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DOI: https://doi.org/10.24198/kultivasi.v22i3.50581