Agronomic biofortification of Calcium in Pak choy (Brassica rapa subsp. chinensis) via hydroponic nutrient film technique
Abstract
Pak choy is rich in calcium, magnesium, phosphorus, iron, and vitamin K, all essential for health. Pak choy requires a proper balance of nutrients for optimal growth. In a hydroponic system, any imbalance in nutrient levels can negatively impact plant growth and reduce the nutritional value stored in the tissues. Selecting suitable varieties and providing appropriate calcium treatment can help increase calcium content and enhance productivity. This study aims to determine the effect of calcium concentration on growth, yield, and Ca content and the most responsive varieties to increased calcium concentration. The research design used was a Split Plot Design with two factors, namely calcium nitrate concentration (k) as the main plot consisting of k0 = without addition of Ca(NO3)2, k1 = addition of 300 mg/L Ca(NO3)2, k2 = addition of 600 mg/L Ca(NO3)2, k3 = addition of 900 mg/L Ca(NO3)2, and k4 = addition of 1200 mg/L Ca(NO3)2. Varieties (v) as subplots consist of v1 = Masbro, v2 = Nauli F1, and v3 = Flamingo. The results showed that the Nauli F1 variety had the best effect on the number of leaves, growth rate index, and fresh weight of shoots. Adding 900 mg/L of calcium nitrate had the best impact on plant growth, while adding 1200 mg/L of calcium nitrate caused plant poisoning. The Masbro variety was responsive to Ca biofortification.
Keywords
Full Text:
PDFReferences
Ahmad P, Latef AAA, Abd-Allah EF, Hashem A, Sarwat M, Anjum NA, Gucel S. 2016. Calcium and potassium supplementation enhanced growth, osmolyte secondary metabolite production, and enzymatic antioxidant machinery in cadmium-exposed chickpea (Cicer arietinum L.). Frontiers in Plant Science, 7: 1–12.
Ardiansyah M, Nugroho B, Sa'diyah K. 2022. Estimating chlorophyll and n content in corn leaves based on chlorophyll content index. Jurnal Ilmu Tanah dan Lingkungan, 24: 53-61.
Aryandhita MI, Kastono D. 2021. Pengaruh pupuk kalsium dan kalium terhadap pertumbuhan dan kualitas hasil sawi hijau (Brassica rapa L.). Vegetalika, 10: 107-112.
Ceccherini GJ, de Lima BM, de Leme LT, Santucci LF, Verruma-Bernardi MR, Purquerio LFV, Sala FC. 2024. Agronomic biofortification of Eruca sativa L. with iron in nutrient film technique hydroponic cultivation. Australian Journal of Crop Science, 18: 145–152.
de Figueiredo MN, Martinez HEP, Fontes EAF, Milagres CDC, Silva JMD. 2022. Agronomic biofortification with iodine in lettuce plants cultivated in floating hydroponic system. Revista Ceres, 69: 210–217.
de Lima BM, Noboa CS, de Lima FM, da Costa MS, Purquerio LFV, Sala FC. 2023. Agronomic biofortification with zinc in hydroponically cultivated lettuce. Australian Journal of Crop Science, 17: 198–205.
Divya R, Sandhya V, Hitesh S. 2021. A review on effect of potassium and calcium on different parameters on plants under hydroponic condition. EPRA International Journal of Research & Development (IJRD), 1: 87–91.
do Moraes GVC, da Silva BH, Silva VFA, da Cunha FF, de Oliveira RA, de Oliveira JT, Silva PA. 2023. Influence of calcium on the development of corn plants grown in hydroponics. Agriengineering, 5: 623–630.
Elena CM, Cámara-Martos F, Obregón S, Rubén BF, de Haro A. 2021. Advances in breeding in vegetable brassica rapa crops. in brassica breeding and biotechnology. Intech Open. Spanish.
El-Beltagi, H. S., and Mohamed, H. I. (2013). Alleviation of cadmium toxicity in Pisum sativum L. seedlings by calcium chloride. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41: 157–168.
Farid N, Ulinnuha Z. 2022. Pertumbuhan dan hasil genotipe bawang merah pada peningkatan dosis sulfur melalui sistem hidroponik nutrient film technique growth and yield of several genotypes of shallots (Allium ascalonicum) on increasing sulfur doses. Biofarm, 18: 102–115.
Gaj R, Budka A, Górski D, Borowiak K, Wolna-Maruwka A, Krzysztof B. 2018. Magnesium and calcium distribution in maize under differentiated doses of mineral fertilization with phosphorus and potassium. Journal of Elementology, 23: 137–150.
Gali´c L, Vinkovi´c T, Ravnjak B and Lonˇcari´c Z. 2021. Agronomic biofortification of significant cereal crops with selenium—A Review, Agronomy 11: 10-15.
Griffiths M, Roy S, Guo H, Seethepalli A, Huhman D, Ge Y, Sharp RE, Fritschi FB, York LM. 2021. A multiple ion-uptake phenotyping platform reveals shared mechanisms affecting nutrient uptake by roots. Plant Physiology, 185: 781–795.
Himschoot E, Beeckman T, Friml J, Vanneste S. 2015. Calcium is an organizer of cell polarity in plants. Biochimica et Biophysica Acta - Molecular Cell Research, 1853: 2168–2172.
Hosseini SA, Réthoré E, Pluchon S, Ali N, Billiot B, Yvin JC. 2019. Calcium application enhances drought stress tolerance in sugar beet and promotes plant biomass and beetroot sucrose concentration. International Journal of Molecular Sciences, 20: 1-22.
Huang W, Shi Y, Yan H, Wang H, Wu D, Grierson D, Chen K. 2023. The calcium-mediated homogalacturonan pectin complexation in cell walls contributes the firmness increase in loquat fruit during postharvest storage. Journal of Advanced Research, 49: 47–62.
Kashtoh H, Baek KH. 2021. Structural and functional insights into the role of guard cell ion channels in abiotic stress-induced stomatal closure. Plants, 10: 1-20.
Li F, He X, Tang M, Tang X, Liu J, Yi Y. 2020. Adaptation of plants to high-calcium content kart regions: Possible involvement of symbiotic microorganisms and underlying mechanisms. Brazilian Journal of Biology, 80: 209–214.
Melcrová A, Pokorna S, Pullanchery S, Kohagen M, Jurkiewicz P, Hof M, Jungwirth P, Cremer PS, Cwiklik L. 2016. The complex nature of calcium cation interactions with phospholipid bilayers. Scientific Reports, 6: 1–12.
Reitz NF, Shackel KA, Mitcham EJ. 2021. Differential effects of excess calcium applied to whole plants vs. excised fruit tissue on blossom-end rot in tomato. Scientia Horticulturae, 290: 1-7.
Sardans J, Peñuelas J. 2021. Potassium control of plant functions: Ecological and agricultural implications. Plants. 10: 1-31.
Seyfferth C, Tsuda K. 2014. Salicylic acid signal transduction: The initiation of biosynthesis, perception and transcriptional reprogramming. Frontiers in Plant Science, 5: 1-10.
Sirohiwal A, Neese F, Pantazis DA. 2019. Microsolvation of the redox-active tyrosine-d in photosystem ii: correlation of energetics with epr spectroscopy and oxidation-induced proton transfer. Journal of the American Chemical Society, 141: 3217–3231.
Suryantini NN, Wijaya G, Dwiyani R. 2020. Pengaruh penambahan Ca(NO3)2 terhadap hasil tanaman selada kriting (Lactuca sativa L.) pada sistem hidroponik deep flow technique (dft). Agrotrop : Journal on Agriculture Science, 10: 190-200.
Thor K. 2019. Calcium—nutrient and messenger. Frontiers in Plant Science, 10: 1-7.
Wang Q, Yang S, Wan S, Li X. 2019. The significance of calcium in photosynthesis. International Journal of Molecular Sciences, 20: 1–14.
Wang Q, Zhang H, Wei L, Guo R, Liu X, Zhang M, Fan J, Liu S, Liao J, Huang Y, Wang Z. 2023. Yellow-green leaf 19 encoding a specific and conservative protein for photosynthetic organisms affects tetrapyrrole biosynthesis, photosynthesis, and reactive oxygen species metabolism in rice. International Journal of Molecular Sciences, 24: 1-26.
Wdowiak A, Podgórska A, Szal B. Calcium in plants: an important element of cell physiology and structure, signaling, and stress responses. Acta Physiologiae Plantarum, 46: 1-20.
Weng X, Li H, Ren C, Zhou Y, Zhu W, Zhang S, Liu L. 2022. Calcium regulates growth and nutrient absorption in poplar seedlings. Frontiers in Plant Science, 13: 1–15.
Yip DT, Boudsocq M. 2019. Properties and functions of calcium-dependent protein kinases and their relatives in Arabidopsis thaliana. New Phytologist, 224: 585–604.
Zhang XP, Ma CX, Sun LR, Hao FS. 2020. Roles and mechanisms of Ca2+ in regulating primary root growth of plants. Plant Signaling and Behavior, 15:1-6.
Zhou Z, Struik PC, Gu J, Peter ELVDP, Wang Z, Yin X, Yang J. 2023. Enhancing leaf photosynthesis from altered chlorophyll content requires optimal partitioning of nitrogen. Crop and Environment 2:24-36.
DOI: https://doi.org/10.24198/kultivasi.v23i3.56760
Refbacks
- There are currently no refbacks.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Kultivasi Indexed by:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.