Impacts of gibberellin application on citrus: An overview from seed germination to fruit quality enhancement
Abstract
Gibberellins are key plant hormones, and their application in citrus plants is frequently reported. This review aims to provide an overview of the application of gibberellic acid (GA3) in citrus cultivation, emphasizing their effects on growth, flowering, fruit quality, and ripening. Citrus fruits often face challenges like irregular fruit set, size inconsistency, and environmental stress, impacting yield and profitability, with exogenous gibberellin application as a potential solution. Applying gibberellins can accelerate citrus seed germination and seedling growth, enhance vegetative growth, inhibit flowering, delay fruit ripening, and improve fruit quality. However, the efficacy of gibberellins varies across species and conditions, highlighting the need for reference studies. This work presents an alternative option for optimizing gibberellin use to support sustainable citrus production practices.
Keywords
Full Text:
PDFReferences
Adhikary T, Chattopadhyay P, Gill SP. 2022. Plant growth regulators in citrus. In: Plant Growth Regulators in Tropical and Sub-tropical Fruit Crops. CRC Press, 113-142.
Adewoyin O, Famaye A, Ipinmoroti R, Ibidapo A, Fayose F. 2023. Postharvest handling methods, processes and practices for pepper. In: Capsicum-Current Trends and Perspectives. IntechOpen.
Agustí M, Reig C, Martínez-Fuentes A, Mesejo C. 2022. Advances in citrus flowering: A review. Frontiers in Plant Science, 13: 868831.
Ahmad MH, Rao MJ, Hu J, Xu Q, Cao Z, Liu CC, Lijun C. 2022. Citrus flowering and fruit setting. In: Citrus Production. CRC Press, 109-125.
Alferez F, de Carvalho DU, Boakye D. 2021. Interplay between abscisic acid and gibberellins, as related to ethylene and sugars, in regulating maturation of non-climacteric fruit. International Journal of Molecular Sciences, 22(2): 669.
Al-Musawi MA, Al-Moussawi SM. 2020. GA3 and Zn impact on germination and seedling growth of acid lime.
Aliyev E, Latif M. 2022. Proceedings of the International Subtropical and Citrus Conference.
Arya SR, Viji MM. 2024. Physiological and Biotechnological Approaches in Enhancing Quality Parameters of Chilli (Capsicum annuum L.). Journal of Advances in Biology & Biotechnology, 27(11): 874-888.
Ayaz H, Shah SS, Khan I, Zaman B, Ali F, Rehman MR, Sufyan A. 2023. Effect of pre-harvest application of GA3 and potassium nitrate on yield and quality of peach fruit. Pakistan Journal of Science, 75(02).
Bacelar E, Pinto T, Anjos R, Morais MC, Oliveira I, Vilela A, Cosme F. 2024. Impacts of climate change and mitigation strategies for some abiotic and biotic constraints influencing fruit growth and quality. Plants, 13(14): 1942.
Bagnazari M, Saidi M, Mohammadi M, Khademi O, Nagaraja G. 2018. Pre-harvest CaCl2 and GA3 treatments improve postharvest quality of green bell peppers (Capsicum annum L.) during storage period. Scientia Horticulturae, 240: 258-267.
Baseer, AQ, Niazi P, Monib AW, Hassand MH, Hejran AB, Sarwari A, Fahmawi, SMS. 2024. Lifecycle Transitions in Plant Development: Ripening, Senescence, & Cell Death. Journal of Pharma Insights and Research, 2(2): 169-179.
Bauerle WL. 2022. Gibberellin A3 induced flowering intensification in Humulus lupulus L.: Synchronizing vegetative phase change and photoperiod induction. Scientia Horticulturae, 302: 111183.
Bennici S, Las Casas G, Distefano G, Gentile A, Lana G, Di Guardo M, Continella A. 2021. Rootstock affects floral induction in citrus engaging the expression of the flowering locus t (Cift). Agriculture, 11(2): 140.
Bermejo A, Granero B, Mesejo C, Reig C, Tejedo V, Agustí M, Iglesias DJ. 2018. Auxin and gibberellin interact in citrus fruit set. Journal of Plant Growth Regulation, 37: 491-501.
Binenbaum J, Weinstain R, Shani E. 2018. Gibberellin localization and transport in plants. Trends in plant science, 23(5): 410-421.
Bisht TS, Rawat L, Chakraborty B, Yadav V. 2018. A recent advances in use of plant growth regulators (PGRs) in fruit crops-A review. International journal of current microbiology and applied sciences, 7(5): 1307-1336.
Budiarto R, Mubarok S, Nanda MA, Nabiyyu M, Jaya MHIS. 2023. The increase in kaffir lime leaf production due to gibberellin is diminished by pruning. Horticulturae, 9(9): 1018.
Bons HK, Kaur N, Rattanpal HS. 2015. Quality and quantity improvement of citrus: role of plant growth regulators. International Journal of Agriculture, Environment and Biotechnology, 8(2): 433-447.
Cai N, Chen C, Wan C, Chen J. 2021. Effects of pre-harvest gibberellic acid spray on endogenous hormones and fruit quality of kumquat (Citrus japonica) fruits. New Zealand Journal of Crop and Horticultural Science, 49(2-3): 211-224.
Castro-Camba R, Sánchez C, Vidal N, Vielba JM. 2022. Plant development and crop yield: The role of gibberellins. Plants, 11(19): 2650.
Chai SK, Ooi SE, Ho CL, Ong-Abdullah M, Chan KL, Fitrianto A, Namasivayam P. 2023. Transcriptomic analysis reveals suppression of photosynthesis and chlorophyll synthesis following gibberellic acid treatment on oil palm (Elaies guineensis). Journal of Plant Growth Regulation, 42(9): 5683-5699.
Chauhan N, Sharma JB, Rana K, Mir W, Bakshi M. 2020. Effects of gibberellins and Promalin on the growth and development of fruit crops: A review. Journal of Pharmacognosy and Phytochemistry, 9(6): 1284-1289.
Croft H, Chen JM, Luo X, Bartlett P, Chen B, Staebler RM. 2017. Leaf chlorophyll content as a proxy for leaf photosynthetic capacity. Global change biology, 23(9): 3513-3524.
Dilip WS, Singh D, Moharana D, Rout S, Patra SS. 2017. Effect of gibberellic acid (GA) different concentrations at different time intervals on seed germination and seedling growth of Rangpur lime. J Agroeco Nat Resource Management, 4: 157-165.
Du M, Zhu Y, Nan H, Zhou Y, Pan X. 2024. Regulation of sugar metabolism in fruits. Scientia Horticulturae, 326: 112712.
Elmenofy H, Kheder A, Mansour A. 2021. Improvement of fruit quality and marketability of “Washington Navel” orange fruit by cytokinin and gibberellin. Egyptian Journal of Horticulture, 48(2): 141-156.
El-Sayed FS. 2018. Effect of some growth regulators and dry yeast on growth of Cleopatra mandarin rootstock seedlings. Middle East J Agric Res, 7: 1301-1309.
Forghani AH, Almodares A, Ehsanpour, AA. 2020. The role of gibberellic acid and paclobutrazol on oxidative stress responses induced by in vitro salt stress in sweet sorghum. Russian journal of plant physiology, 67: 555-563.
Garmendia A, Beltrán R, Zornoza C, Garcia-Breijo FJ, Reig J, Merle H. 2019. Gibberellic acid in Citrus spp. flowering and fruiting: A systematic review. PloS One, 14(9): e0223147.
Ge N, Jia JS, Yang L, Huang RM, Wang QY, Chen C, Chen JW. 2023. Exogenous gibberellic acid shortening after-ripening process and promoting seed germination in a medicinal plant Panax notoginseng. BMC Plant Biology, 23(1): 67.
Gill K, Kumar P, Kumar A, Kapoor B, Sharma R, Joshi AK. 2022. Comprehensive mechanistic insights into the citrus genetics, breeding challenges, biotechnological implications, and omics-based interventions. Tree Genetics & Genomes, 18(2): 9.
Goldschmidt EE, Sadka A. 2021. Yield alternation: horticulture, physiology, molecular biology, and evolution. Horticultural Reviews, 48: 363-418.
Gomaa, A. M. (2020). Productivity and fruit quality of lime (Citrus aurantifolia L.) as affected by GA3 and NAA foliar spray. Hortscience Journal of Suez Canal University, 9(1): 63-71.
Griebeler SR, Gonzatto MP, Böettcher GN, Schneider LA, Sulzbach M, Gargioni ED, Schwarz SF. 2021. Successive applications of gibberellic acid to reduce flowering of 'Montenegrina' mandarin in alternate bearing. Pesquisa Agropecuária Brasileira, 56: e02303.
Hazarika TK. 2023. Citrus. In: Fruit and Nut Crops. Springer Nature Singapore, 1-44.
Hedden P, Sponsel V. 2015. A century of gibberellin research. Journal of plant growth regulation, 34: 740-760.
Hedden P, Thomas SG. 2016. Annual Plant Reviews, The Gibberellins (Vol. 49). John Wiley & Sons. New Jersey.
Huber M, Nieuwendijk NM, Pantazopoulou CK, Pierik R. 2021. Light signalling shapes plant–plant interactions in dense canopies. Plant, Cell & Environment, 44(4): 1014-1029.
Huchche AD, Ladaniya MS. 2014. Souvenir-Physiology of Flowering in Perennial Fruit Crops. Proceedings of the National Seminar-Cum-Workshop on Physiology of Flowering in Perennial Fruit Crops, 74-88.
Hung NQ, My Ha LT, Hieu NQ, Tu PTT, Lam VP. 2023. Gibberellin (GA3) and Copper Sulfate Pentahydrate (CuSO4• 5H2O) Reduce Seeds per Fruit and Increase Fruit Quality in Bac Son Mandarin Fruit. Seeds, 2(3) 318-330.
Kalatippi AS, SS P, Patil K, Dongre R, Kuldeep DK, Bhooriya MS. 2024. Citrus physiological disorders and their ameliorating control measures: A review. Journal of Scientific Research and Reports, 30(5): 56-69.
Keawmanee N, Ma G, Zhang L, Yahata M, Murakami K, Yamamoto M, Kato M. 2022. Exogenous gibberellin induced regreening through the regulation of chlorophyll and carotenoid metabolism in Valencia oranges. Plant Physiology and Biochemistry, 173: 14-24.
Khalil HA. 2020. Improved yield, fruit quality, and shelf life in ‘Flame Seedless’ grapevine with pre-harvest foliar applications of forchlorfenuron, gibberellic acid, and abscisic acid. Journal of Horticultural Research, 28(1): 77-86.
Khan MN, Nabi G. 2024. The potential role of gibberellic acid in regulating photosynthetic pigments, fruit quality and antioxidant enzymes in sweet lime Citrus limetta Risso. Pakistan Journal of Botany, 57(2).
Khopkar RR, Nagaharshitha D, Haldavanekar PC, Parulekar YR. 2017. Studies on seed germination of Pummelo (Citrus grandis L. Osbeck). International Journal of Agricultural Sciences and Research, 7: 257-264.
Kumara KT, Singh H, Kaur N, Kang BK, Devi I. 2023. Uniconazole improves mango flowering and fruit yield by regulating gibberellins and carbon–nitrogen nutrition. Horticulture, Environment, and Biotechnology, 64(5): 735-752.
Kupke BM, Tucker MR, Able JA, Porker KD. 2022. Manipulation of barley development and flowering time by exogenous application of plant growth regulators. Frontiers in plant science, 12: 694424.
Lee SY, Park K, Jang BK, Ji B, Lee H, Baskin CC, Cho JS. 2022. Exogenous gibberellin can effectively and rapidly break intermediate physiological dormancy of Amsonia elliptica seeds. Frontiers in Plant Science, 13: 1043897.
Livingston T, Vashisth T. 2022. Timing of gibberellic acid applications to manipulate flowering and improve fruit production in HLB-affected sweet orange. In: XIV International Symposium on Plant Bioregulators in Fruit Production. 1344: 137-142.
Lurie S. 2024. Manipulating fruit development and storage quality using growth regulators. Plant Growth Regulators in Agriculture and Horticulture, 1: 175-196.
Martínez C, Espinosa‐Ruiz A, Prat S. 2016. Gibberellins and plant vegetative growth. Annual Plant Reviews, Volume 49: Gibberellins, 285-322.
Mesejo C, Yuste R, Reig C, Martínez-Fuentes A, Iglesias DJ, Muñoz-Fambuena N, Agustí M. 2016. Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species. Plant Science, 247: 13-24.
Meshram PC, Joshi PS, Bhoyar RK, Sahoo AK. 2015. Effect of different plant growth regulators on seedling growth of acid lime. Res. Envrn. Life Sci, 8(4): 725-728.
Meshram PC, Joshi PS, Bhoyar RK, Sahoo AK. 2023. Effect of foliar application of growth regulators on growth, flowering and yield of guava (Psidium guajava L.). Indian Journal of Horticulture, 80(3): 353-359.
Miransari M, Smith DL. 2014. Plant hormones and seed germination. Environmental and experimental botany, 99: 110-121.
Mishra S, Srivastava V, Mehrotra S, Quadri, SN. 2017. The regulation of plant development: cross‐talk of reactive oxygen species and plant hormones. Reactive Oxygen Species in Plants: Boon Or Bane‐Revisiting the Role of ROS, 243-260.
Mukherjee A, Fritschi FB, Putta V, Schoonhoven A, Hasegawa K, Faria O. 2022. Genotype and environmental effects on gibberellin levels in soybeans. Plant Physiology, 189(3): 1393-1405.
Neto FJD, dos Santos Carneiro DC, Putti FF, Rodrigues JD, Tecchio MA, Leonel S, de Souza Silva M. 2024. Physiological Indexes in Seed Germination and Seedling Growth of Rangpur Lime (Citrus limonia L. Osbeck) under Plant Growth Regulators. Agronomy, 14(9): 2066.
Ni J, Gao C, Chen MS, Pan BZ, Ye K, Xu ZF. 2015. Gibberellin promotes shoot branching in the perennial woody plant Jatropha curcas. Plant and Cell Physiology, 56(8): 1655-1666.
Niharika, Singh NB, Singh A, Khare S, Yadav V, Bano C, Yadav RK. 2021. Mitigating strategies of gibberellins in various environmental cues and their crosstalk with other hormonal pathways in plants: a review. Plant Molecular Biology Reporter, 39: 34-49.
Ochiki SN, Chen T, Meng Z, Zhou J, Gao Z, Deng Y, Luan M. 2024. Characterization of ATP-PFK genes during ripening and their modulation by gibberellic acid and salicylic acid phytohormones during postharvest storage of citrus fruits (Citrus reticulata Blanco.). Plant Physiology and Biochemistry, 109235.
Pereira CS, Siqueira DLD, Valiati S, Ferrari E. 2014. Application of GA3 and girdling of branches on the production of extemporaneous fruits of'Tahiti'acid lime. Revista Ceres, 61: 970-974.
Rahman MM, Khan ABMMM, Hasan MM, Banu LA, Howlader MHK. 2018. Effect of foliar application of gibberellic acid on different growth contributing characters of mungbean. Progressive Agriculture, 29(3): 233-238.
Rai O, Patil SN, Patil DR, Venkatshalu, Awati M, Kiran KC. 2018. Effect of plant growth regulators and chemical on vegetative and reproductive parameters during hasta bahar in acid lime (Citrus aurantifolia Swingle). Int. J. Curr. Microbiol. App. Sci, 7(9): 2640-2650.
Raveh E, Goldenberg L, Porat R, Carmi N, Gentile A, La Malfa S. 2020. Conventional breeding of cultivated citrus varieties. The citrus genome, 1: 33-48.
Rezk A, Pervaiz T, Douhan G, Obenland D, Arpaia ML, El-Kereamy A. 2024. Preharvest Mandarin rind disorder: insights into varietal differences and preharvest treatments effects on postharvest quality. Plants, 13(8): 1040.
Rokaya PR, Baral DR, Gautam DM, Shrestha AK, Paudyal KP. 2016. Effect of pre-harvest application of gibberellic acid on fruit quality and shelf life of mandarin (Citrus reticulata Blanco). American Journal of Plant Sciences, 7(07): 1033.
Shah SH, Islam S, Mohammad F, Siddiqui MH. 2023. Gibberellic acid: a versatile regulator of plant growth, development and stress responses. Journal of Plant Growth Regulation, 42(12): 7352-7373.
Sharaf MM, Atawia AR, Bakry KA, El-Rouby MZ. 2016. Effect of pre-sowing seeds soak in different GA3 and ZnSO4 solutions on germination and growth of Cleopatra mandarin and Rangpur lime rootstocks. Middle East J Agric Res, 5: 233-238.
Shinozaki Y, Beauvoit BP, Takahara M, Hao S, Ezura K, Andrieu MH, Ariizumi T. 2020. Fruit setting rewires central metabolism via gibberellin cascades. Proceedings of the National Academy of Sciences, 117(38): 23970-23981.
Singh G, Rattanpal HS, Gupta M, Sidhu GS. 2022. Genetic variability and heritability among mandarin (Citrus reticulata Blanco) genotypes under Indian sub-tropical conditions. Applied Ecology & Environmental Research, 20(4).
Talat H, Shafqat W, Qureshi MA, Sharif N, Raza MK, ud Din S, ... Jaskani MJ. 2020. Effect of gibberellic acid on fruit quality of Kinnow mandarin. J Glob Innov Agri Soc Sci, 8(2): 59-63.
Thirugnanasambantham K, Prabu G, Mandal AKA. 2020. Synergistic effect of cytokinin and gibberellins stimulates release of dormancy in tea (Camellia sinensis (L.) O. Kuntze) bud. Physiology and molecular biology of plants, 26(5): 1035-1045.
Vashisth T, Kadyampakeni D. 2020. Diagnosis and management of nutrient constraints in citrus. Fruit crops, 49: 723-737.
Vishal B, Kumar PP. 2018. Regulation of seed germination and abiotic stresses by gibberellins and abscisic acid. Frontiers in plant science, 9: 838.
Wang J, Yao Y, Wang Y, Li X, Yang C. 2023. The interaction of gibberellins and auxin in fruit set of Citrus reticulata Blanco. Journal of Horticultural Research, 31(1): 89-101.
Wang W, Hu X, Zhao Y, Zeng X. 2024. Effects of gibberellin and 6-benzylaminopurine on flowering and fruit quality of ‘Satsuma’ mandarin. Horticultural Science, 51(2): 215-223.
Wu B, Sun M, Zhang H, Yang, D, Lin C, Khan I, Huang L. 2021. Transcriptome analysis revealed the regulation of gibberellin and the establishment of photosynthetic system promote rapid seed germination and early growth of seedling in pearl millet. Biotechnology for Biofuels, 14: 1-14.
Xiong M, Feng GN, Gao Q, Zhang CQ, Li QF, Liu QQ. 2022. Brassinosteroid regulation in rice seed biology. Seed Biology, 1(1): 1-9.
Yamaga I, Tonooka C, Emoto Y. 2024. A comprehensive analysis of gibberellic acid and prohydrojasmon treatments for mitigating rind puffing and rind disorder of early-maturing cultivar of satsuma mandarin. Horticulture, Environment, and Biotechnology, 1: 1-10.
Zhang J, Cao Y, Tang J, He X, Li M, Li C, ... Ding Y. 2023. Physiology and application of gibberellins in postharvest horticultural crops. Horticulturae, 9(6): 625.
Zhang M, Lin Z, Huang Y, Wu Z. 2021. The effects of exogenous gibberellin on flowering, fruiting, and fruit quality in persimmon. Journal of the Science of Food and Agriculture, 101(11): 4737-4743.
Zhang W, Liu S, Wang J, Zhang Y, Zhao Y, Han H. 2022. Role of gibberellin in promoting flowering and fruiting in strawberry. Journal of Plant Growth Regulation, 41(1): 212-226.
Zhu GL, Yin J, Guo XQ, Chen XB, Zhi WF., Liu JW, ... & Zhou GS. 2019. Gibberellic acid amended antioxidant enzyme and osmotic regulation to improve salt tolerance of okra at early growth stage. International Journal of Agriculture and Biology, 22(2): 270-276.
DOI: https://doi.org/10.24198/kultivasi.v23i3.58796
Refbacks
- There are currently no refbacks.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Kultivasi Indexed by:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.