An overview of gibberellin inhibitors for regulating vegetable growth and development

Erik Setiawan, Rahmat Budiarto, Jajang Sauman Hamdani, Mansour Ghorbanpour

Abstract


Gibberellin (GAs) promotes plant growth processes like germination, shoot elongation, root development, and flowering, while its inhibition is occasionally used in some vegetable crop practices. This paper aims to review the current literature on GAs inhibitors in practical vegetable crops and suggest future strategies for increasing yield. In some vegetable crops, inhibiting GAs with natural (abscisic acid, ethylene) or synthetic (paclobutrazol, prohexadione-Ca) regulators is key to improving yield and quality. Abscisic acid (ABA) counteracts GAs in germination and stress adaptation, while ethylene (ET) opposes GAs in senescence and growth inhibition. The application of paclobutrazol (PBZ) and prohexadione-Ca (Pro-Ca) is known to inhibit synthetic GAs, resulting in denser plants while improving plant photosynthetic efficiency, which improves crop yield. Achieving desired growth and yields with GAs inhibitors relies on precise dosing, emphasizing the importance of review studies for advancing vegetable cultivation.


Keywords


abscisic acid; ethylene; paclobutrazol; prohexadione-Ca; plant growth

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Agboola DA, Ogunyale OG, Fawibe OO, Ajiboye AA. 2014. A review of plant growth substances: Their forms, structures, synthesis and functions. Journal of Advanced Laboratory Research in Biology, 5(4): 152–168.

Ahmad I, Kamran M, Ali S, Cai T, Bilegjargal B, Liu T, Han Q. 2018. Seed filling in maize and hormones crosstalk regulated by exogenous application of uniconazole in semiarid regions. Environmental Science and Pollution Research, 25: 33225–33239.

Albertos P, Romero-Puertas MC, Tatematsu K, Mateos I, Sánchez-Vicente I, Nambara E, Lorenzo O. 2015. S-nitrosylation triggers ABI5 degradation to promote seed germination and seedling growth. Nature Communications, 6(1): 8669.

Ali F, Qanmber G, Li F, Wang Z. 2022. Updated role of ABA in seed maturation, dormancy, and germination. Journal of Advanced Research, 35: 199-214.

Altuntaș Ö. 2016. The effects of Prohexadione-Ca treatments on plant growth, nutrient elements uptake and fruit quality in tomato, Yüzüncü Yil Üniversitesi Journal of Agricultural Sciences, 26(1): 98-105.

An ZF, Zhou CJ. 2017. Light induces lettuce seed germination through promoting nitric oxide production and phospholipase D-derived phosphatidic acid formation. South African Journal of Botany, 108: 416–422.

Assaf M, Korkmaz A, Karaman Ş, Kulak M. 2022. Effect of plant growth regulators and salt stress on secondary metabolite composition in Lamiaceae species. South African Journal of Botany, 144: 480–493.

Bai Q, Huang Y, Shen Y. 2021. The physiological and molecular mechanism of abscisic acid in regulation of fleshy fruit ripening. Frontiers in Plant Science, 11: 619953.

Bar M, Ori N. 2014. Leaf development and morphogenesis. Development, 141: 4219–4230. doi: 10.1242/dev.106195.

Başak H. 2021. Effects of Prohexadione calcium applications on growth and yield characteristics of cucumber (Cucumis sativus L.). Sains Malaysiana, 50(8): 2141–2152.

Bethke PC, Libourel IG, Aoyama N, Chung YY, Still DW, Jones RL. 2007. The Arabidopsis aleurone layer responds to nitric oxide, gibberellin, and abscisic acid and is sufficient and necessary for seed dormancy. Plant Physiology, 143(3): 1173–1188.

Brookbank BP, Patel J, Gazzarrini S, Nambara E. 2021. Role of basal ABA in plant growth and development. Genes, 12(12): 1936.

Cai K, Zhu S, Jiang Z, Xu K, Sun X, Li X. 2024. Biological macromolecules mediated by environmental signals affect flowering regulation in plants: A comprehensive review. Plant Physiology and Biochemistry, 214: 108931.

Carrera-Castaño G, Calleja-Cabrera J, Pernas M, Gómez L, Oñate-Sánchez L. 2020. An updated overview on the regulation of seed germination. Plants, 9(6): 703

Cázarez Flores LL, Velázquez Alcaraz TDJ, Partida Ruvalcaba L, Díaz Valdés T, Ayala Tafoya F, Zazueta Torres ND, Yáñez Juárez MG. 2018. Paclobutrazol applied on cotyledonal leaves and quality of cucumber, squash, melon and watermelon seedlings. Agricultural Sciences, 2018(9): 264-271

Chahtane H, Kim W, Lopez-Molina L. 2017. Primary seed dormancy: a temporally multilayered riddle waiting to be unlocked. Journal of Experimental Botany, 68(4): 857-869.

Chen S, Wang XJ, Tan GF, Zhou WQ, Wang GL. 2020. Gibberellin and the plant growth retardant Paclobutrazol altered fruit shape and ripening in tomato. Protoplasma, 257: 853-861.

Chen P, Yang R, Bartels D, Dong T, Duan H. 2022. Roles of abscisic acid and gibberellins in stem/root tuber development. International Journal of Molecular Sciences, 23: 4955.

Chen X, Gao Z, Li Y, Nie X, Xie Q, Chen G, Hu Z. 2024. Knockdown of SlYTHDF2 accelerates dark-induced tomato leaf senescence by affecting the ABA pathway. Plants, 13(19): 2800.

Cline JA. 2017. Interactive effects of 6-BA, GA4+7, and prohexadione-calcium on Gala apples. Can J Plant Sci, 97(4): 632-644. https://doi.org/10.1139/cjps-2016-0314.

Corbineau F. 2024. Ethylene, a Signaling Compound Involved in Seed Germination and Dormancy. Plants, 13(19): 2674.

Darmawan M, Poerwanto R, Susanto S. 2014. The application of prohexadione-Ca, paclobutrazol, and strangulation for off-season flowering induction of mandarin citrus plants (Citrus reticulata). Journal of Horticulture, 24(2): 133–140.

Desta B, Amare G. 2021. Paclobutrazol as a plant growth regulator. Chemical Biology and Technology in Agriculture, 8: 1-15.

Dewi K, Agustina RZ, Nurmalika F. 2016. Effects of blue light and paclobutrazol on seed germination, vegetative growth, and yield of black rice (Oryza sativa L. 'Cempo Ireng'). Biotropia, 23(2): 85–96.

Diwan G, Sahu MK, Tiwari S, Ahirwar K, Haldar A, Rangare NR, Banjare K, Kumar S, Ahirwar MK, Samaiya RK, Lal N. 2022. Effect of new plant growth regulator prohexadione-Ca on horticultural crops. Agricultural Mechanization in Asia, 53: 9671-9681.

El-Maarouf-Bouteau H, Sajjad Y, Bazin J, Langlade N, Cristescu SM, Balzergue S, Baudouin E, Bailly C. 2015. Reactive oxygen species, abscisic acid and ethylene interact to regulate sunflower seed germination. Plant, Cell & Environment, 38(2): 364-374.

Fenn MA, Giovannoni JJ. 2021. Phytohormones in fruit development and maturation. The Plant Journal, 105: 446-458.

Groot SP, Karssen CM. 1992. Dormancy and germination of abscisic acid-deficient tomato seeds: studies with the sitiens mutant. Plant Physiology, 99(3): 952–958.

Guo Y, Ren G, Zhang K, Li Z, Miao Y, Guo H. 2021. Leaf senescence: progression, regulation, and application. Molecular Horticulture, 1: 1-25.

Hamdani JS, Budiarto R, Ramadani SF. 2024. Retardant Improve Seed Tuber Yield of G0 Potato (Solanum tuberosum cv. Medians). Asian Journal of Plant Sciences, 23: 118-122.

Hamdani JS, Dewi TP, Sutari W. 2019. Pengaruh komposisi media tanam dan waktu aplikasi zat pengatur tumbuh terhadap pertumbuhan dan hasil benih kentang Solanum tuberosum L. G2 kultivar medians di dataran medium Jatinangor. Kultivasi, 18(2): 875–881.

Hamdani JS, Nuraini A, Mubarok S. 2018. The use of paclobutrazol and shading net on growth and yield of potato 'Medians' tuber of G2 in medium land of Indonesia. Asian Journal of Plant Sciences, 23: 118-122.

Harris JM. 2015. Abscisic acid: hidden architect of root system structure. Plants, 4(3): 548–572.

Hedden P, Sponsel V. 2015. A century of gibberellin research. Journal of plant growth regulation, 34: 740-760.

Hernawati D, Hamdani JS, Mubarok S, Sumekar Y, Alfiya M. 2022. Physiological response, yield and quality of GO potato (Solanum tuberosum) seed yield to various concentrations and time of application of prohexadione-ca bioregulator. Research Crops, 23(3): 640-648.

Hoang HH, Sechet J, Bailly C, Leymarie J, Corbineau F. 2014. Inhibition of germination of dormant barley (Hordeum vulgare L.) grains by blue light as related to oxygen and hormonal regulation. Plant, Cell & Environment, 37(6): 1393-1403.

Huang X, Shi H, Hu Z, Liu A, Amombo E, Chen L, Fu J. 2017. ABA is involved in regulation of cold stress response in bermudagrass. Frontiers in Plant Science, 8: 1613.

Huang X, Hou L, Meng J, You H, Li Z, Gong Z, Shi Y. 2018. The antagonistic action of abscisic acid and cytokinin signaling mediates drought stress response in Arabidopsis. Molecular Plant, 11(7): 970-982.

Humplík JF, Bergougnoux V, Van Volkenburgh E. 2017. To stimulate or inhibit? That is the question for the function of abscisic acid. Trends in Plant Science, 22(10): 830-841.

Iqbal N, Khan NA, Ferrante A, Trivellini A, Francini A, Khan MIR. 2017. Ethylene role in plant growth, development, and senescence: interaction with other phytohormones. Frontiers in Plant Science, 8: 475.

Ito A, Sakamoto D, Itai A, Nishijima T, Oyama-Okubo N, Nakamura Y, Moriguchi T, Nakajima I. 2016. Effects of GA3+4 and GA4+7 application either alone or combined with prohexadione-Ca on fruit development of Japanese pear ‘Kosui’. Horticulture Journal, 85(3): 201-208.

Kai W, Wang J, Liang B, Fu Y, Zheng Y, Zhang W, Leng P. 2019. PYL9 is involved in the regulation of ABA signaling during tomato fruit ripening. Journal of Experimental Botany, 70(21): 6305-6319.

Kang J, Yim S, Choi H, Kim A, Lee KP, Lopez-Molina L, Martinoia E, Lee Y. 2015. Abscisic acid transporters cooperate to control seed germination. Nature Communications, 6(1): 8113.

Karabulut F, Faizan M, Rasheed D, Ahmad Z, Unnisa G, Faraz A. 2024. Integration of plant hormones in the biological system as an opportunity for sustainable crop. Plant Growth Regulators, 243.

Khan S, Alvi AF, Khan NA. 2024. Role of Ethylene in the Regulation of Plant Developmental Processes. Stresses, 4(1): 28-53.

Khandaker MM, Syafiq M, Abdulrahman MD, Mohd KS, Yusoff N, Sajili MH, Badaluddin NA. 2020. Influence of paclobutrazol on growth, yield and quality of eggplant (Solanum melongena). Asian Journal of Plant Sciences, 19(4): 361-371.

Kong D, Ju C, Parihar A, Kim S, Cho D, Kwak JM. 2015. Arabidopsis glutamate receptor homolog3. 5 modulates cytosolic Ca2+ level to counteract effect of abscisic acid in seed germination. Plant physiology, 167(4) 1630-1642.

Kou X, Yang S, Chai L, Wu C, Zhou J, Liu Y, Xue Z. 2021. Abscisic acid and fruit ripening: Multifaceted analysis of the effect of abscisic acid on fleshy fruit ripening. Scientia Horticulturae, 281: 109999.

Kristina N, Gustian G, Yusniwati Y, Khairunnisa N. 2024. Effect of concentration and time of giving paclobutrazol on Lumbu Hijau-Garlic seedling bulbs. AGRIUM: Jurnal Ilmu Pertanian, 27(1): 1-9.

Kępczyński J, Sznigir P. 2014. Participation of GA3, ethylene, NO and HCN in germination of Amaranthus retroflexus L. seeds with various dormancy levels. Acta Physiologiae Plantarum, 36: 1463-1472.

Lee J, Gómez MI, Miller WB. 2015a. Paclobutrazol and flurprimidol control stem elongation of potted star of Bethlehem. HortTechnology, 25(4): 480-486.

Lee K, Lee HG, Yoon S, Kim HU, Seo PJ. 2015b. The Arabidopsis MYB96 transcription factor is a positive regulator of ABSCISIC ACID-INSENSITIVE4 in the control of seed germination. Plant Physiology, 168(2): 677-689.

Li X, Chen L, Forde BG, Davies WJ. 2017. The biphasic root growth response to abscisic acid in Arabidopsis involves interaction with ethylene and auxin signalling pathways. Frontiers in plant science, 8: 1493.

Liu T, Longhurst AD, Talavera-Rauh F, Hokin SA, Barton MK. 2016. The Arabidopsis transcription factor ABIG1 relays ABA-signaled growth inhibition and drought-induced senescence. Elife, 5: e13768.

Mabvongwe O, Manenji BT, Gwazane M, Chandiposha M. 2016. The effect of paclobutrazol application time and variety on growth, yield, and quality of potato (Solanum tuberosum L.). Advances in Agriculture, 2016(1): 1585463.

Malik NU, Fajer O, Amin L, Khalid AR, Khan N, Bhatti MF, Munir F, Haider G, Amir R, Gul A. 2023. Phytohormones, plant growth and development. In Phytohormones and Stress Responsive Secondary Metabolites (pp. 175-186). Academic Press.

Nonogaki H. 2017. Seed biology updates–highlights and new discoveries in seed dormancy and germination research. Frontiers in Plant Science, 8: 524.

Novita A. 2022. The effect of Gibberellin (GA3) and Paclobutrazol on growth and production on Tomato (Lycopersicum esculentum Mill.). In IOP Conference Series: Earth and Environmental Science, 1025( 1): 012037. IOP Publishing.

Nurrachman N, Muthahanas I, Azhari AP, Wangiyana W. 2023. Growth and Yield Responses of Chili (Capsicum frutescens L.) to Paclobutrazol Concentrations and P-Fertilizer Doses during the Rainy Season. International Journal of Environment, Agriculture and Biotechnology, 8(3): 86-90.

Nuraini A, Rochayat Y, Widayat D. 2016. Rekayasa source–sink dengan pemberian zat pengatur tumbuh untuk meningkatkan produksi benih kentang di dataran medium desa Margawati

Ozbay N, Ergun N. 2015. Prohexadione calcium on the growth and quality of eggplant seedlings. Pesquisa Agropecuária Brasileira, Brasília, 50(10): 932-938.

Özbay N, Metın R. 2016. Prohexadione-calcium affects vegetative growth and yield of pepper. VII International Scientific Agriculture Symposium, "Agrosym 2016", 6-9 October 2016, Jahorina, Bosnia and Herzegovina. Proceedings, 2016: 446-453.

Pane HYBT, Hanafiah DS, Damanik RI. 2021. Effect of Paclobutrazol concentration on initial formation stolon in potato plants by in vitro at different temperature conditions. In IOP Conference Series: Earth and Environmental Science, 782(4): 042052. IOP Publishing.

Pan W, Liang J, Sui J, Li J, Liu C, Xin Y, Zhang Y, Wang S, Zhao Y, Zhang J, Yi M, Gazzarrini S, Wu J. 2021. ABA and bud dormancy in perennials: current knowledge and future perspective. Genes, 12(10): 1635.

Park J, Lee Y, Martinoia E, Geisler M. 2017. Plant hormone transporters: what we know and what we would like to know. BMC Biology, 15: 1-15.

Qin H, Zhang Z, Wang J, Chen X, Wei P, Huang R. 2017. The activation of OsEIL1 on YUC8 transcription and auxin biosynthesis is required for ethylene-inhibited root elongation in rice early seedling development. PLoS Genetics, 13(8): e1006955.

Ramos-Fernández J, Ayala-Garay OJ, Pérez-Grajales M, Sánchez-del Castillo F, Magdaleno-Villar JJ. 2020. Effect of paclobutrazol on plant growth, yield and fruit quality in tomato. Horticulturae, 33(1): 59-64.

Rana N, Singh S, Dhakad AK, Dhatt KK. 2023. Coding phenological growth stages of yellow bells (Tecoma stans (L.) Juss. ex Kunth) based on BBCH scale and its implications for urban greening. Current Plant Biology, 34: 100284.

Reid MS, Wu MJ. 2018. Ethylene in flower development and senescence. In The Plant Hormone Ethylene. CRC Press.

Sami A, Riaz MW, Zhou X, Zhu Z, Zhou K. 2019. Alleviating dormancy in Brassica oleracea seeds using NO and KAR1 with ethylene biosynthetic pathway, ROS and antioxidant enzymes modifications. BMC Plant Biology, 19: 1-15.

Sano N, Marion-Poll A. 2021. ABA metabolism and homeostasis in seed dormancy and germination. International Journal of Molecular Sciences, 22(10): 5069.

Santos JAS, Garcia QS. 2023. Ethylene in the regulation of seed dormancy and germination: Biodiversity matters. In The Plant Hormone Ethylene (pp. 61-71). Academic Press.

Sarker SD, Nahar L. 2022. Characterization of nanoparticles. In Das Talukdar A, Dey Sarker S, Patra JK, editors. Advances in Nanotechnology-Based Drug Delivery Systems, Nanotechnology in Biomedicine. Elsevier, Amsterdam, Netherlands, pp. 45–82. ISBN 978-0-323-88450-1.

Sarker BC, Rahim MA, Archbold DD. 2016. Combined effects of fertilizer, irrigation, and paclobutrazol on yield and fruit quality of mango. Horticulturae, 2(4): 14.

Sharma V, Gantait A, Kapil D. 2024. Advances in Gibberellic Acid Application in Cropping. International Journal of Plant and Soil Science, 36(8): 344-357.

Shu K, Liu XD, Xie Q, He ZH. 2016. Two faces of one seed: hormonal regulation of dormancy and germination. Molecular plant, 9(1): 34-45.

Shu K, Zhou W, Chen F, Luo X, Yang W. 2018. Abscisic acid and gibberellins antagonistically mediate plant development and abiotic stress responses. Frontiers in Plant Science, 9: 416.

Shtin M, Ioio, RD, Del Bianco M. 2022. It’s time for a change: The role of gibberellin in root meristem development. Frontiers in Plant Science, 13: 882517.

Singh V, Sergeeva L, Ligterink W, Aloni R, Zemach H, Yang J, Zhang P, Shabtai S, Firon N. 2019. Gibberellin promotes sweet potato root vascular lignification and reduces storage-root formation. Frontiers in Plant Science, 10: 1320.

Singh G, Saini A, Thakur Y, Batish DR, Sharma A. 2024. Efficacy of Click derived 1,2,3-Bistriazole on selective recognition of Co (II) and its application in seed germination. Journal of Molecular Structure, 135: 138806.

Sun X, Shu J, Mohamed AAM, Deng X, Zhi X, Bai J, Cui Y, Lu X, Du Y, Wang X, Huang Z, Guo Y, Liu L, Li J. 2019. Identification and characterization of EI (Elongated Internode) gene in tomato (Solanum lycopersicum). International Journal of Molecular Sciences, 20(9): 2204.

Sun LR, Wang YB, He SB, Hao FS. 2018. Mechanisms for abscisic acid inhibition of primary root growth. Plant signaling & behavior, 13(9): e1500069.

Tang M, Zhao W, Xing M, Zhao J, Jiang Z, You J, Ni B, Ni Y, Liu C, Li J, Chen X. 2021. Resource allocation strategies among vegetative growth, sexual reproduction, asexual reproduction, and defense during the growing season of Aconitum kusnezoffii Reichb. The Plant Journal, 105(4): 957–977.

Tuan PA, Kumar R, Rehal PK, Toora PK, Ayele BT. 2018. Molecular mechanisms underlying abscisic acid/gibberellin balance in the control of seed dormancy and germination in cereals. Frontiers in plant science, 9: 668.

Wang GL, Que F, Xu ZS, Wang F, Xiong AS. 2015. Exogenous gibberellin altered morphology, anatomic, and transcriptional regulatory networks of hormones in carrot root and shoot. BMC Plant Biology, 15: 1-12.

Wang Y, Zhao J, Lu W, Deng D. 2017. Gibberellin in plant height control: old player, new story. Plant Cell Reports, 36: 391-398.

Xie Q, Essemine J, Pang X, Chen H, Cai W. 2020. Exogenous application of abscisic acid to shoots promotes primary root cell division and elongation. Plant Science, 292: 110385.

Xu Q, Krishnan S, Merewitz E, Xu J, Huang B. 2016. Gibberellin-regulation and genetic variations in leaf elongation for tall fescue in association with differential gene expression controlling cell expansion. Scientific Reports, 6(1): 30258.

Yalamalle VR, Arunachalam T, Kumari R, Ithape DM, Ghosh S, Singh M. 2020. Ethephon reduces lodging and enhances seed yield and quality in onion. International Journal of Bio-Resource and Stress Management, 11(6): 601-606.

Zhang S, Gottschalk C, van Nocker S. 2019. Genetic mechanisms in the repression of flowering by gibberellins in apple (Malus x domestica Borkh.). BMC Genomics, 20: 1-15.

Zhang Y, Li Q, Jiang L, Kai W, Liang B, Wang J, Leng P. 2018. Suppressing type 2C protein phosphatases alters fruit ripening and the stress response in tomato. Plant and Cell Physiology, 59(1): 142-154.

Zhang R, Zheng D, Feng N, Qiu QS, Zhou H, Meng F, Huang X, Huang A, Li Y. 2023. Prohexadione-calcium alleviates the leaf and root damage caused by salt stress in rice (Oryza sativa L.) at the tillering stage. PLoS One, 18(3): e0279192.

Zhang J, Zhou T, Zhang C, Zheng W, Li J, Jiang W, Xiao C, Wei D, Yang C, Xu R, Gong A, Bi Y. 2021. Gibberellin disturbs the balance of endogenous hormones and inhibits adventitious root development of Pseudostellaria heterophylla through regulating gene expression related to hormone synthesis. Saudi Journal of Biological Sciences, 28(1): 135-147.

Zheng S, Su M, Shi Z, Gao H, Ma C, Zhu S, Zhang T. 2022. Exogenous sucrose influences KEA1 and KEA2 to regulate abscisic acid-mediated primary root growth in Arabidopsis. Plant Science, 317: 111209.




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