Effect of meta-topolin and kinetin at various concentrations on shoot multiplication in white turmeric (Curcuma zedoaria Rosc.)
Abstract
White turmeric is a medicinal plant widely used to treat various diseases. The limited availability of white turmeric seedlings is influenced by the scarcity of quality seeds due to the long dormancy of the rhizomes and the high incidence of pathogen attacks. Tissue culture techniques are one alternative to address the problem of white turmeric seedling availability. This study aims to determine the best concentrations of meta-topolin and kinetin for in vitro shoot multiplication of white turmeric. The research was conducted from January to June 2022 at the tissue culture laboratory, Faculty of Agriculture, Universitas Padjadjaran. This study used a completely randomized design consisting of 7 treatments: control (0 mg L-1), meta-topolin (1 mg L-1; 2 mg L-1; 3 mg L-1), kinetin (1 mg L-1; 2 mg L-1; 3 mg L-1). Analysis of variance (ANOVA) and Duncan’s multiple range test were used to analyze the statistical effects of meta-topolin and kinetin application. Applying 1 mg L-1 meta-topolin yielded the best results for shoot number, indicating that 1 mg L-1 meta-topolin is a potential cytokinin for the propagation of Curcuma zedoaria Rosc.
Keywords
Full Text:
PDFReferences
Maxiselly Y, Humaira DS, Sari DN, Suherman C. 2023. Morpho-physiological traits and phytochemical compositions of Coffea canephora beans from Lampung for various harvesting stages and soaking durations. International Journal of Plant Biology, 14: 746-754.
Budiarto R, Poerwanto R, Santosa E, Efendi D, Agusta A. 2023. Agro-physiological traits of kaffir lime in response to pruning and nitrogen fertilizer under mild shading. Plants, 12: 1-14.
Ahmad N, & Strnad M. 2021. Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture. Springer Nature, Singapore.
Al-Zubaidi BAQ., Makki BED, Al-Rubaie F. 2020. Effect of citric acid on some morphological characteristics and the content of major nutrient of chamomile (Matricaria chamomilla L.) at increasing concentrations of kinetin. Plant Archives, 20(1): 592–596.
Arnon D. I. 1949. Copper enzymes in isolated chloroplast, Polyphenoloxidase in Beta vulgari. Plant Physiol, 24(1): 1–15.
Badan Pusat Statistik. 2020. Produksi Hortikultura Tanaman Biofarmaka Provinsi Jawa Barat. Badan Pusat Statistik, Jakarta.
Batubara I, Kartika Y, Darusman L. K. 2016. Relationship between Zingiberaceae leaves compounds and its tyrosinase activity. Biosaintifika: Journal of Biology & Biology Education, 8(3): 371.
Bella DRS, Suminar E, Nuraini A, Ismail A. 2016. pengujian efektifitas berbagai jenis dan konsentrasi sitokinin terhadap multiplikasi tunas mikro pisang (Musa paradisiaca L.) secara In Vitro. Jurnal Kultivasi. 15(2): 74 – 80.
Bimantara DS., Maghfoer D, Barunawati N, Syahrian A. 2018. Multiplikasi kultur meristem stroberi kultivar earlibrite dengan pengambahan konsentrasi hormon BAP dan kinetin. J. Prod. Tanam. 6(3): 432–437.
Budiono R, Setiawati T, Pitaloka GG, Anggreini L, Nurzaman M, Mutaqin AZ. 2016. Mikropropagasi stroberi (Fragaria x ananassa Var. earlibrite) dengan penambahan BA (Benzyl adenine) dan IBA (Indole butyric acid) pada media MS (murashige and skoog). Pros. Semin. Nas. II Tahun 2016(3): 1126–1138.
Chuengpanya R, Chuenboonngarm N, Sakchaichanchol K, Muangkroot A, Thammasiri K. 2020. In vitro propagation and callus induction of Hedychium longicornutum Griff. ex Baker using different explants. Acta Horticulturae, 1298: 323–330.
Dwiyan, R. 2015. Kultur Jaringan Tanaman. Pelawa Sari, Yogyakarta.
Espinosa-Leal CA, Puente-Garza CA, García-Lara S. 2018. In vitro plant tissue culture: means for production of biological active compounds. Planta, 248(1): 1–18.
Falster DS, & Westoby M. 2003. Plant height and evolutionary games. Trends in Ecology and Evolution, 18(7): 337–343.
Gawande S, Nagre P, Nikam C. 2020. Effect of planting material and plant growth regulators on growth of turmeric. International Journal of Chemical Studies, 8(6): 198–201.
Geismann TA. 1962. The Chemistry of Flavonoid Counpound. New York: Pergamon Press Oxford.
Gentile A, Jàquez Gutiérrez M, Martinez J, Frattarelli A, Nota P, Caboni E. 2014. Effect of meta-Topolin on micropropagation and adventitious shoot regeneration in Prunus rootstocks. Plant Cell, Tissue and Organ Culture, 118(3): 373–381.
Haque SM, & Ghosh B. 2018. Micropropagation of Kaempferia angustifolia Roscoe - an aromatic, essential oil yielding, underutilized medicinal plant of Zingiberaceae family. Journal of Crop Science and Biotechnology, 21(2): 147–153.
Hashiguchi A, San Thawtar M, Duangsodsri T, Kusano M, Watanabe KN. 2022. Biofunctional properties and plant physiology of Kaempferia spp.: status and trends. Journal of Functional Foods, 92(2): 105029.
Heriansyah P, & Jumin HB. 2020. In-vitro rooting induction on the embryo somatic of Dendrobium. PASPALUM : Jurnal Ilmiah Pertanian, 8(2): 93–98.
Khandaker MM, Liyana N, Majrashi A, Dalorima T, Alias N, Mat N. 2018. Effects of kinetin on the morpho-physiological and biochemical characteristics of stevia (Stevia rebaudiana). Bulgarian Journal of Agricultural Science, 24(4): 638–647.
Krishna AG, Ansary P, Oommen S. M. 2020. Kaempferia rotunda Linn.-phytochemical profile. International Research Journal of Pharmacy and Medical Sciences, 3(6): 21–24.
Krishnakumar P, Varghese M, Joe MG, Rajagopal A, Varghese L. 2021. Identification and bioactivities of endophytic fungi from Lagenandra toxicaria dalz. and kaempferia rotunda L. Journal of Applied Biology and Biotechnology, 9(4): 117–125.
Li Q, Wang H, Wang H, Zheng W, Wu D, Wang Z. 2018. Effects of kinetin on plant growth and chloroplast ultrastructure of two Pteris species under arsenate stress. Ecotoxicology and Environmental Safety, 158(12): 37–43.
Mawaddah SK, Saputro NW, Lestari A. 2021. Pemberian naphthalene acetic acid (NAA) dan kinetin terhadap multiplikasi tunas tanaman jahe (Globba leucantha var. bicolor Holttum) pada kultur In vitro. Bioma : Berkala Ilmiah Biologi, 23(1): 43–50.
Morzadec JM, & Hourmant A. 1997. In vitro rooting improvement of globe artichoke (cv. Camus de Bretagne) by GA3. Scientia Horticulturae, 72(1): 59–62.
Munadi E, Rose S, Salim Z. 2021. Delivering the vision. In Learning Disability Practice, 5(4).
Naznin S, Ferdous M, Uddin MS., Yeasmin F, Daula ASU, Basher MA. 2019. Phytochemical investigation and evaluation of antioxidant activities of methanolic extract of Globba marantina leaves. Journal of Medicinal Plants Studies, 7(6): 147–153.
Novita I, Miftah H, Sunaryo MA. 2020. Preferensi konsumen dalam pembelian obat herbal kunyit putih. Jurnal Agribisains, 6(2): 81–88.
Prabhukarthikeyan SR, Manikandan R, Durgadevi D, Keerthana U, Harish S, Karthikeyan G, Raguchander T. 2017. Bio-suppression of turmeric rhizome rot disease and understanding the molecular basis of tripartite interaction among Curcuma longa, Pythium aphanidermatum and Pseudomonas fluorescens. Biological Control, (111): 23–31.
Pramanik D, Shintiavira H, Winarto B. 2018. Studi kualitas regeneran phalaenopsis hasil kultur in vitro dari eksplan tangkai infloresen, tunas pucuk, dan empulur. Jurnal Hortikultura, 28(1): 13-24.
Rodinah, Nina C, Rohmayanti E. 2012. Inisiasi pisang talas (Musa paradisiaca var sapientum L.) dengan pemberian sitokinin secara in vitro. Agroscientiae 19(2): 107-111.
Salvi ND, George L, Eapen S. 2002. Micropropagation and field evaluation of micropropagated plants of turmeric Plant Cell, Tissue and Organ Culture, 68(2): 153-151.
Seow-neng C, Bakar NA, Mahmood M, Chai-ling H. 2017. Crop Improvement. Crop Improvement, (10): 253–270.
Setiawati T, Ayalla A, Nurzaman M, Kusumaningtyas VA, Bari I. 2020. Analysis of secondary metabolites of shoot, callus culture and field plant of Chrysanthemum morifolium Ramat. Jurnal Ilmu Dasar, 21(1): 1.
Spíchal L, Rakova N, Riefler M. (2004). Two cytokinin receptors of Arabidopsis thaliana, CRE1/AHK4 and AHK3, differ in their ligand specificity in a bacterial assay. Plant Cell Physiol, 45(9): 1299–1305
Van der Westhuizen A. 2014. The use of meta-topolin as an alternative cytokinin in the tissue culture of Eucalyptus species. Acta Horticulturae, 1055(1): 25–28.
Wahyudi E, Ernita, Faturrahman. 2013. Uji konsentrasi kinetin dan naa terhadap multiplikasi embrio aren (Arenga pinnata (W) Merr) secara In vitro. Jurnal Dinamika Pertanian, 28(1): 51–62.
Waman AA, Bohra P, Devi K, Pixy J. 2021. In vitro multiplication protocol for Curcuma mangga: Studies on carbon, cytokinin source and explant size. Journal of Horticultural Sciences, 16(1): 69–76.
Wybouw B, De Rybel B. 2019. Cytokinin – A developing story. Trends in Plant Science, 24(2): 177–185.
Yaowachai W, Saensouk S, Saensouk P. 2020. In vitro propagation and determination of total phenolic compounds, flavonoid contents and antioxidative activity of Globba globulifera gagnep. Pharmacognosy Journal, 12(6): 1740–1747.
Yasin NA, Khan WU, Ahmad SR, Ali A, Ahmad A, Akram W. 2018. Imperative roles of halotolerant plant growth-promoting rhizobacteria and kinetin in improving salt tolerance and growth of black gram (Phaseolus mungo). Environmental Science and Pollution Research, 25(5): 4491–4505.
Zulkarnain Z. 2018. Pengaruh IAA dan BAP terhadap proliferasi kalus serta vitrifikasi dan nekrosis pucuk pada kultur jaringan Guichenotia macrantha TURCZ. Buletin Agronomi Universitas Jambi, 1: 11-15.
DOI: https://doi.org/10.24198/kultivasi.v24i1.61664
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Kultivasi Indexed by:
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.