Effect of ultraviolet light irradiation on the growth of Candida albicans and the transversal strength of acrylic plate

Teguh Tri Widodo; Rachman Ardan; Eddy Prijono

doi:10.24198/pjd.vol22no3.26897

Effect of ultraviolet light irradiation on the growth of Candida albicans and the transversal strength of acrylic plate

Teguh Tri Widodo, Rachman Ardan, Eddy Prijono

Abstract

Introduction: Acrylic plates are the basis for several tools used in the oral cavity, including dentures and obturators. In its function, the acrylic plate is always in contact with the oral mucosa, so that good disinfection is needed without reducing the strength of the acrylic plate. Disinfection can be done by ultraviolet irradiation. This study is aimed to analyze the effect of ultraviolet light irraying on the growth of Candida albicans and the strength of transversal acrylic plate. Methods: This research is an experimental laboratory research with Replication Random Design. The disinfection success test was carried out by observing the population of the Candida albicans colony, while the acrylic plate transversal strength test was carried out on 35 acrylic heat curing plates tested according to ADA specifications. Results: Ultraviolet light can inhibit the growth of Candida albicans colonies with an optimum irradiation time of 20 minutes at a wavelength of 254nm. Meanwhile, ultraviolet irradiation on acrylic plates between 5-30 minutes shows a significant transverse strength. Conclusion: Ultraviolet light at appropriate wavelengths and times has been shown to significantly inhibit bacterial colony growth and significantly increase the transverse strength of acrylic plates.

Keywords

Ultraviolet light, irradiation, Candida albicans, transversal strength, acrylic plate.

References

Tortora GJ, Funke BR, Case CL. Microbiology an Introductions. 7th ed. Philadelpia: Benjamin Cummings. An Imprint of Addison Wesley Longman Inc. 2001. p. 12129.

Nichols RL. The Operating Room. In: Benneth JV, Brachman PS. Hospital Infection. 4th ed. Philadelpia: Lipincott-Raven. 1998. p. 421-30.

Fisher JE, Fegelman E, Joharmigman. 1999. Surgical complication. In: Schwartz SI, Shires GT, Spencer FC, Daly JM, Fisher JE, Galloway AC. Principles of Surgery. 7th ed. Vol 1. New York: Mc Graw-Hill. p. 448-51.

Ishida H, Nahara Y, Tammamoto M, Hamada T. The fungicidal effect of ultraviolet light on impression materials. J Prosthet Dent. 1991;65:532-7.

Boylan RJ, Goldstein GR, Schulman A. Evaluation of an ultraviolet unit. Prosthet Dent. 1987;58(4): 635-50.

Mahrus F. Pengaruh bahan pembersih sabun terhadap basis gigi tiruan lepasan. J PDGI. 1993;42(3):72-4.

Connors JNE. An evaluation of an enzyme denture cleanser. J Prosthet Dent. 1977;37(2):147-57.

Budtz-Jorgensen E. Clinical aspects of Candida albicans infection in denture wearers. J Prosthet Dent. 1978;42(6):619-23.

Shen C. Strength of denture repairs as influence by surface treatment. J Prosthet Dent. 1984;52(6):844-8.

Bhattacharya I, Cohen DM, Silverman S. Jr. Red and White Lesions of the Oral Mucosa. In: Greenberg M, Glick M. Burket’s Oral Medicine Diagnosis and Treatment. London: BC Decker Inc; 2011. p. 85-119.

Craig RG. Restorative Dental Materials. 11th ed. St. Louis: Mosby Company; 2002. p. 643-4.

Rossalina E. Pengaruh Perendaman Akrilik Heat Cured dalam Sodium Hipoklorit Terhadap Kekuatan Transversal. Skripsi. Bandung: Fakultas Kedokteran Gigi Universitas Padjadjaran; 2002. p. 82-9.

Shen C, Colaizzi FA, Birns B. Strength of denture repairs as influenced by surface treatment. J Prosthet Dent. 1984;52(6):844-8.

Annusavice KJ. Phillip’s Science of Dental Material. 6th ed. Philadelpia: WB Saunders Co; 1996. p. 230-50.

Munadziroh E, Indrasari M. Biokompabilitas bahan basis gigi tiruan resin akrilik. Dent J (Maj Ked Gi). 2000;7:116-20.

Rastogi RP, Richa, Kumar A, Tyagi MB, Sinha RP. Molecular mechanisms of ultraviolet radiation-induced dna damage and repair. J Nucleic Acids. 2010;2010:592980.

Truman RW, Gillis TP. The effect of ultraviolet light radiation on Mycobacterium leprae. Int J Lepr Other Mycobact Dis. 2000;68(1):11-7.

Friedberg EC, Walker GC, Siede W, Wood RD. DNA and Mutagenesis. 2nd ed. Washington DC: American Society for Microbiology Press; 2005:

Ketchum PA. Microbiology: Concepts and Application. 1st ed. Hoboken: Wiley; 1988.

Moat AG, Foster JW. Microbial Physiology. 3rd ed. Hoboken: John Wiley & Sons, Inc; 1996.

Kurtz MB, Marrinan J. Isolation of hem3 mutants from Candida albicans by sequential gene disruption. Mol Gen Genet. 1989;217(1):47-52.

Kochi JK, Wiley J. Handbook of Free Radical Initiators. J A Howard in Free Radicals. 1973;2:3.

Phillips RW, Skinner EW. Skinner’s science of dental materials. 9th ed. Philadelphia: Saunders-Elsevier; 1991.

Mattos BSC, de Sousa AA, de Magalhaes MHCG, Andre M, Brito R, Dias. Candida albicans in patients with oronasal communication and obturator prostheses. Braz Dent J. 2009;20(4):336-40.

Lung CY, Darvell BW. Minimization of the inevitable residual monomer in denture base acrylic. Dent Mater. 2005;21(12):1119-28.

Combe EC. Notes and Dental Material. 6th ed. London: Churchill Livingstone; 1992.

DOI: https://doi.org/10.24198/pjd.vol22no3.26897