Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of mangosteen (Garcinia mangostana Linn.) rind extract against Aggregatibacter actinomycetemcomitans

Ina Hendiani, Agus Susanto, Dyah Nindita Carolina, Rahmat Ibrahim, Felisha Febriane Balafif

Abstract


Introduction: The main contents of mangosteen (Garcinia mangostana Linn.) is xanthone with antibacterial activity. Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) was a gram-negative, facultative anaerobic bacillus that causes periodontal diseases such as localised aggressive periodontitis (LAP). Mangosteen rind extract could inhibit the growth of Porphyromonas gingivalis and A. actinomycetemcomitans. This study was aimed to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of mangosteen rind extract against A. actinomycetemcomitans. Methods: This study was an experimental and in-vitro study of antimicrobial properties of mangosteen (Garcinia mangostana Linn.).The extract of mangosteen was made by the maceration method using 70% ethanol solvent. A. actinomycetemcomitans (ATCC 43718) obtained from Laboratorium of Microbiology Airlangga University Surabaya. This study used a microdilution method and analysed using the Enzyme Linked Immune Sorbent Assay (ELISA). The determination of the MIC was measured using the formula for cell inhibition percentage. Three concentrations higher than the MIC standard were cultured on the Mueller Hinton agar. The lowest concentration on agar which was not found any growth of the bacterial colonies was determined as the MBC. Results: The results of this study indicated that the MIC and MBC of mangosteen rind extract against A. actinomycetemcomitans were 195.3 ppm and 1562.5 ppm, respectively. Conclusion: Mangosteen rind extract can inhibit and bactericide the growth of A. actinomycetemcomitans.


Keywords


Antibacterial; Aggregatibacter actinomycetemcomitans; mangosteen rind extract; xanthone

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References


Yang R, Li P, Li N, Zhang Q, Bai X, Wang L, et al. Xanthones from the pericarp of Garcinia man-gostana. Molecules. 2017; 22(5): 683–93. DOI: 10.3390/molecules22050683

Gutierrez-Orozco F, Failla ML. Biological activities and bioavailability of mangosteen xanthones: A critical review of the current evidence. Nutrients. 2013; 5(8): 3163-83. DOI: 10.3390/nu5083163

Lim TK. Edible Medicinal and Non-Medicinal Plants: Volume 11 Modified Stems, Roots, Bulbs. New York: Springer; 2012. pp. 83-100.

Shan T, Ma Q, Guo K, Liu J, Li W, Wang F, et al. Xanthones from mangosteen extracts as natural chemopreventive agents: Potential anticancer drugs. Curr Mol Med. 2011; 11(8): 666‑77. DOI: 10.2174/156652411797536679

Torrungruang K, Vichienroj P, Chutimaworapan S. Antibacterial activity of mangosteen pericarp extract against cariogenic Streptococcus mutans. CU Dent J. 2007; 30: 1-10.

Nguyen PTM, Marquis RE. Antimicrobial actions of a-mangostin against oral Streptococci. Can J Microbiol. 2011; 57(3): 217-25. DOI: 10.1139/W10-122

Aizat WM, Jamil IN, Ahmad-Hashim FH, Noor NM. Recent updates on metabolite composition and medicinal benefits of mangosteen plant. PeerJ. 2019; 7: e6324. DOI: 10.7717/peerj.6324

Pribadi N, Yonas Y, Saraswati W. The inhibition of Streptococcus mutans glucosyltransferase enzyme activity by mangosteen pericarp extract. Dent J (Maj Ked Gi). 2017; 50(2): 97–101. DOI: 10.20473/j.djmkg.v50.i2.p97-101

Sodata P, Juntavee A, Juntavee N, Peerapattana J. Optimization of AdhesivePastes for dental caries prevention. AAPS PharmSciTech. 2017; 18(8): 3087–96. DOI: 10.1208/s12249-017-0750-0

Gholizadeh P, Pormohammad A, Eslami H, Shokouhi B, Fakhrzadeh V, Kafil HS. Oral pathogenesis of Aggregatibacter actinomycetemcomitans. Microb Pathog. 2017; 113: 303-11. DOI: 10.1016/j.micpath.2017.11.001

Shah R, Gayathri GV, Mehta DS. Application of herbal products in management of periodontal dis-eases: A mini review. Int J Oral Health Sci. 2015; 5(1): 38-44. DOI: 10.4103/2231-6027.171166

Hendiani I, Hadidjah D, Susanto A, Pribadi IMS. The effectiveness of mangosteen rind extract as additional therapy on chronic periodontitis (Clinical trials). Padjadjaran J Dent. 2017; 29(1): 64-70. DOI: 10.24198/pjd.vol29no1.12986

Hendiani I, Hadidjah D, Susanto A, Pribadi IMS. Inhibitor and bactericidal power of mangosteen rind extract towards Porphyromonas gingivalis and Actinobaccillus actinomycetemcomitans (Laboratory test). Padjadjaran J Dent. 2016; 28(2): 75-80. DOI: 10.24198/pjd.vol28no2.13605

Poeloengan M, Praptiwi P. Uji aktivitas antibakteri kulit buah manggis (Garcinia mangostana Linn). Media Health Res Dev. 2010; 20(2): 65-9.

Azhar IS, Kresnoadi U, Rahayu RP. Potency of Garcinia mangostana L rind extract combined with demineralized freeze-dried bovine bone xenograft on IL-1β expression, osteoblast, and osteoclasts in alveolar bone. Dent J (Maj Ked Gi). 2017; 50(3): 166-170. DOI: 10.20473/j.djmkg.v50.i3.p166-170

Suhartati R, Apriyani F, Khusnul, Virgianti DP, Fathurohman M. Antimicrobial activity test of Mangosteen leaves ethanol extract (Garcinia mangostana Linn) against Pseudomonas aeruginosa bacteria. J Phys. 2019; 1179: 012167. DOI: 10.1088/1742-6596/1179/1/012167

Dharmaratne HR, Sakagami Y, Piyasena KGP, Thevanesam V. Antibacterial activity of xanthones from Garcinia mangostana (l.) and their structure-activity relationship studies. Nat Prod Res. 2013; 27(10): 938-41. DOI: 10.1080/14786419.2012.678348

Geetha R, Roy A, Lakshmi T. Evaluation of antibacterial activity of fruit rind extract of Garcinia mangostana Linn on enteric phatogens-an in vitro study. Asian J Pharm Clin Res. 2011; 4(2): 115-8.




DOI: https://doi.org/10.24198/pjd.vol32no2.27366

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