In vivo histomorphological evaluation of geopolymer-carbonated apatite nanocomposites implanted on rabbit tibia  at early bone healing

Dahlia Sutanto; Mieke Hemiawati Satari; Bethy Suryawathy Hernowo; Bambang Pontjo Priosoeryanto; Rifki Septawendar; Lia Amelia Tresna Wulan Asri; Bambang Sunendar Purwasasmita

doi:10.24198/pjd.vol33no1.28899

In vivo histomorphological evaluation of geopolymer-carbonated apatite nanocomposites implanted on rabbit tibia at early bone healing

Dahlia Sutanto, Mieke Hemiawati Satari, Bethy Suryawathy Hernowo, Bambang Pontjo Priosoeryanto, Rifki Septawendar, Lia Amelia Tresna Wulan Asri, Bambang Sunendar Purwasasmita

Abstract

Introduction: Dental implants have become a more desirable treatment for replacing missing teeth. The mechanical properties and biocompatibility of titanium and zirconia are excellent, but they are less bioactive. The chemical composition of the carbonate apatite is similar to enamel and dentin. Geopolymers are inorganic polymers, and they are similar to ceramics. They have excellent mechanical properties, bioactivity, biocompatibility. The purpose of this study was to assess histomorphological evaluation of geopolymer-carbonated apatite nanocomposites implanted on rabbit tibia at early bone healing in vivo. Methods: Geopolymer-CHA nanocomposites with a diameter of 3 mm and length of 6 mm was placed in the tibia of eight male New Zealand White rabbit whose body weight is 3 to 3.5 kg and six-month ages. Experimental subjects were randomly assigned to 2 groups for assessing the bone healing capability around samples to 14 and 28 days histomorphologically. Wilcoxon test was performed, and p<0.05 was considered significant, using Minitab software version 13. Results: Granulation tissue, woven, and lamellar bone was analysed. A reactive bone formation was revealed in the 14^th day. Osteoblasts, osteoids, and osteocytes showed more mature and woven bone became denser on the 28^th day. Conclusion: Geopolymer-CHA nanocomposites could be considered a candidate for dental implant material from this histomorphological evaluation.

Keywords

histomorphological; geopolymer-CHA nanocomposites; dental implant; early bone healing

Full Text:

PDF

References

Carlo S Di, Angelis F De, Brauner E, Rosella D, Papi P, Pompa G, et al. Histological and immunohistochemical evaluation of mandibular bone tissue regeneration. Int J Immunopathol Pharmacol. 2018;32:1–8.

Villar CC, Huynh-ba GUY, Mills MP, Cochran DL. Wound healing around dental implants. Endodontic Topics. 2012;25:44–62.

Colombo JS, Satoshi S, Okazaki J, Crean S, Sloan AJ, Waddington RJ. In vivo monitoring of the bone healing process around different titanium alloy implant surfaces placed into fresh extraction sockets. J Dent. 2012;40(4):338–46.

Almola BH, Al-ghaban N, Taher A. Deposition at amelogenin coated Ti implant surface. Smile Dental Journal. 2016;9(1):12-17.

Saini M, Singh Y,Arora P, Arora V JK. Implant biomaterial: A comprehensive review. World J Clin Cases. 2015;3(1):52–7.

Shi J, Li Y, Gu Y, Qiao S, Zhang X, Lai H. Effect of titanium implants with strontium incorporation on bone apposition in animal models : A systematic review and meta- analysis. Scientific Reports. 2017; (11):1–10.

Apratim A, Eachempati P, Salian KKK, Singh V, Chhabra S, Shah S. Zirconia in dental implantology: A review. J Int Soc Prev Community Dent. 2015;5(3):147-56.

Chun KJ, Lee JY. Comparative study of mechanical properties of dental restorative materials and dental hard tissues in compressive loads. Journal of Dental Biomechanics. 2014;(5):1–6.

Cui WF, Liu N, Qin GW. Microstructures , mechanical properties and corrosion resistance of the Zr e x Ti ( Ag ) alloys for dental implant application. Materials Chemistry and Physics. 2016; 1-6

Zhang BGX, Myers DE, Wallace GG, Brandt M. Choong PFM. Bioactive coatings for orthopaedic implants — Recent trends in development of implant coatings. Int J Mol Sci. 2014;15:11878–921.

Wang H, Li H, Yan F. Synthesis and mechanical properties of metakaolinite-based geopolymer. Colloids and Surfaces A: Physicochem.Eng.Aspects. 2005;268(9):1–6.

Catauro M, Bollino F, Papale F, Lamanna G. Investigation of the sample preparation and curing treatment effects on mechanical properties and bioactivity of silica rich metakaolin geopolymer. Mater Sci Eng. 2014;(12):20–4.

Sauffi AS, Ibrahim WMW, Mustafa M, Abdulla MMAB, Ahmad R, Zaidi FA,et al. A review of carbonate minerals as an additive to geopolymer materials. Mater Sci Eng. 2019; 551:1-5.

Chen L, Wang Z, Wang Y, Feng J. Preparation and Properties of Alkali Activatedmetakaolin-based geopolymer. Materials.2016;9 (9):1–12.

Rovnanik P. Effect of curing temperature on the development of hard structure of metakaolin- based geopolymer. Constr Build Mater. 2014;24(7):1176–83.

Herwani, Pane I, Imran I, Budiono B. Compressive strength of fly ash-based geopolymer concrete with a variable of sodium hydroxide (NaOH) solution molarity. MATEC Web Conf. 2018; 147:1-5.

Blaszczynski TZ, Krol MR. Alkaline activator impact on the geopolymer binders. Mater Sci Eng. 2017;245: 1–11.

Tippayasam C, Sutikulsombat S, Kamseu E, Rosa R, Thavorniti P, Chindaprasirt P, Leonelli C, Hennes G, Chaysuwan D. In vitro surface reaction in SBF of a non-crystalline aluminosilicate (geopolymer) material. Aust Ceram Soc. 2018;(6):1–7.

Catauro M, Bollino F, Kansal I, Kamseu E, Lancellotti I, Leonelli C. Mechanical and biological characterization of geopolymers for potential application as biomaterials. Azo J Mater. 2012; (5):1–15.

Eliaz N, Metoki N. Calcium phosphate bioceramics : A review of their history, structure, properties, coating technologies and biomedical applications. Materials. 2017; (3):1-104.

Jonge LTD, Leeuwenburgh SCG, Wolke JGC, Jansen JA. Organic – inorganic surface modifications for titanium implant surfaces. Pham.Res. 2008;25(10):1-13.

Müller P, Bulnheim U, Diener A, Lüthen F, Teller M, Klinkenberg ED, et al. Calcium phosphate surfaces promote osteogenic differentiation of mesenchymal stem cells. .J Cell Mol Med. 2008;12(1):281–91.

Barradas AMC, Fernandes HAM, Groen N, Chai YC, Schrooten J, Peppel JVD, et al. A calcium-induced signaling cascade leading to osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells. Biomaterials. 2012;33(1):1-11.

Ohgushi H, Caplan AI. Stem cell technology and bioceramics : from cell to gene engineering. 1999;913–27.

Marsell R, Einhorn TA, The biology of fracture healing,. NIH Public Access. 2012;42(6):551–5.

Terheyden H, Lang NP. Bierbaum S, Stadlinger B. Osseointegration – communication of cells. Clin Oral Impl Res.2011;23:1127–35.

Loi F, Córdova LA, Pajarinen J, Lin T, Yao Z, Goodman SB. Inflammation , fracture and bone repair. Bone. 2016;86(3):119–30.

Ghiasi MS, Chen J, Vaziri A, Rodriguez EK, Nazarian A. Bone fracture healing in mechanobiological modeling : A review of principles and methods. Bone Reports. 2017;6(3):87–100.

Chun KJ, Choi HH, Lee JY. Comparison of mechanical property and role between enamel and dentin in human teeth. J Dent Biomech. 2014;6(2):1–7.

Marshall GW, Marshall SJ, Kinney JH BM. The dentin substrate:structure and properties related to bonding. J Dent. 1997;25(6):441–58.

Kanasan N, Adzila S, Rahman HA, Bano N, Panerselvan G, Hidayati NA. FTIR and XRD evaluation of magnesium doped hydroxyapatite / sodium alginate powder by precipitation method. Key Eng Mater. 2018;791:45–9.

Rehman I, Bonfield W. Characterization of hydroxyapatite and carbonated apatite by photo acoustic FTIR spectroscopy. J Mater Sci Mater Med. 1997;8:1–4.

Chug A, Shukla S, Mahesh L, Jadwani S. Osseointegration-molecular events at the bone-implant interface: A review. J Oral Maxillofac Surgery, Med Pathol. 2013;25(2):1–4.

Shapiro F, Wu JY. Woven bone overview : Structural classification based on its integral role in developmental, repair and pathological bone formation throughout vertebrate groups. European Cells and Materilas.2019;38:137–67.

Groot KD, Geesink R, Klein CPAT, Serekian P. Plasma sprayed coatings of hydroxylapatite. J Biomed Mater Res. 1987;21:1375–81.

Geesink RTG, De Groot K, Klein CPAT. Chemical implant fixation using hydroxyl-apatite coatings. Clin.Orthop.1987;225:147-169.

Toquet J, Rohanizadeh R, Guicheux J, Passuti N, Couillaud S, Passuti N, et al. Osteogenic potential in vitro of human bone marrow cells cultured on macroporous biphasic calcium. J Biomed Mater Res.1999; 44: 98-108.

Kawai N, Niwa S, Sato M, Sato Y, Suwa Y, Ichihara I. Bone formation by cells from femurs cultured among three-dimensionally arranged hydroxyapatite granules. J Biomed Mater Res. 1997;37:1–8.

Urist MR, Lietze A, Dawson E. Beta tricalcium phosphat delivery system for bone morphogenic protein. Clin Orthop. 1984;187:277-80.

Yoshinari M, Oda Y, Inoue T, Matsuzaka K, Shimono M. Bone response to calcium phosphate-coated and bisphosphonate- immobilized titanium implants. Biomaterials. 2002;23:2879–85.

Ramazanoglu M, Oshida Y. Osseointegration and bioscience of implant surface-current concepts at bone-implant interface. In: Implant Dentistry - A Rapidly Evolving Practice p. 57–82. [cited 2020 July 20] Available from: http://www.intechopen.com/books/implant-dentistry-a-rapidly-evolving-practice/osseointegration-and-bioscience-of-implant-surface-current-at-bone-implant-interface

DOI: https://doi.org/10.24198/pjd.vol33no1.28899