Analysis of   FOXE1  rs4460498 and   GSTP-1 I105V   associated with non syndromic cleft lip and palate among Deutero Malay Subrace in Indonesia

Saskia Lenggogeni Nasroen; Florence Meliawaty; Tichvy Tammama; Putri Indriani Prasdiaz; Tias Choirunnisa; Ani Melani Maskoen

doi:10.24198/pjd.vol34no3.39296

**Analysis of FOXE1 rs4460498 and GSTP-1 I105V associated with non syndromic cleft lip and palate among Deutero Malay Subrace in Indonesia**

Saskia Lenggogeni Nasroen, Florence Meliawaty, Tichvy Tammama, Putri Indriani Prasdiaz, Tias Choirunnisa, Ani Melani Maskoen

Abstract

ABSTRACT

Introduction: FOXE1 rs4460498 and GSTP-1 I105V gene polymorphisms are suspected of having a role in some of the non-syndromic cleft lip and palate (NS CLP) populations worldwide. This study aims to analyze FOXE1 rs4460498 and GSTP-1 I105V polymorphisms associated with NS CLP as the risk factor among Deutero Malay Subrace in Indonesia. Methods: This study was a case-control design, using samples from the venous blood of 102 NS CLP subjects and 102 healthy control subjects. After DNA was extracted, the PCR-RFLPs method was performed using TasI restriction enzyme on 100 blood samples of FOXE1 rs4460498 group and Alw26I restriction enzyme on 105 blood samples of the GSTP-1 I105V group. The Chi-Square test was used with the Kolmogorov Smirnov and Exact Fisher alternatives. Results: T mutant allele (OR= 0.926, p>0.05) and CT genotype (OR= 0.0, p>0.05) of FOXE1 rs4460498 and the G mutant allele (OR= 0.988,p>0.05) and AG genotype (OR= 0.675,p>0.05) of the GSTP-1 I105V are not the risks of NS CLP. Conclusion: FOXE1 rs4460498 and GSTP-1 I105V gene polymorphisms are not associated with non-syndromic cleft lip and palate among Deutero Malay Subrace in the Indonesian population.

Key words: deutero malay; FOXE1 rs4460498; GSTP-1 I105V; NS CLP

Keywords

deutero malay; FOXE1 rs4460498; GSTP-1 I105V; NS CLP

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DOI: https://doi.org/10.24198/pjd.vol34no3.39296