The effect of oligosaccharide chitosan irrigating agent on penetration of two type endodontic sealers into the dentinal tubules: a confocal laser scanning microscopy study

Trimurni Abidin; Gieska Lailarahma Darmawan; Fatimah Dewi Dalimunthe

doi:10.24198/pjd.vol34no3.39692

The effect of oligosaccharide chitosan irrigating agent on penetration of two type endodontic sealers into the dentinal tubules: a confocal laser scanning microscopy study

Trimurni Abidin, Gieska Lailarahma Darmawan, Fatimah Dewi Dalimunthe

Abstract

ABSTRACT

Introduction: Irrigation during root canal treatment is one of the important factors that play a role in determining the quality of sealer penetration into the dentinal tubules, especially in the apical third of the tooth root. This is inseparable from the selection of the used irrigation agent. Currently, oligosaccharide chitosan has been developed which is easier to manipulate as an alternative irrigation agent. This study aimed to analyze the effect of the chitosan oligosaccharide irrigating agent on the penetration of two types of endodontic sealers into the dentinal tubules. Methods: This study used 60 mandibular premolars which were randomly divided into 3 groups (n=3). Group 1 was irrigated with 2% chitosan oligosaccharides; Group 2 with a combination of 2.5% NaOCl and 0.2% chitosan nanoparticles; and group 3 was irrigated with a combination of 2.5% NaOCl and 17% EDTA. At the time of obturation, each group was divided into two groups, for sealer treatment using CeraSeal Bioceramic and AH Plus sealer. The sealer’s penetration depth was measured using a confocal laser scanning microscope. Results: The highest mean on the penetration depth of the sealer was seen in the irrigation group using chitosan oligosaccharide solution with CeraSeal bioceramic sealer (2575,151 AU) (p>0.05). Conclusion: There is an effect of 2% oligosaccharide chitosan solution on sealer penetration into dentinal tubules. CeraSeal bioceramic sealer showed better penetration than AH Plus sealer.

Keywords: oligosaccharide chitosan; ceraseal bioceramic sealer; AH plus sealer; confocal laser scanning microscope

Keywords

oligosaccharide chitosan; ceraseal bioceramic sealer; AH plus sealer; confocal laser scanning microscope

Full Text:

PDF

References

REFERENCES

Cohenca N. Disinfection of Root Canal Systems The Treatment of Apical Periodontitis. 1st Ed, UK: John Willey Blackwell. 2014. p. 110-89.

Ratih DN, Sari NI, Santosa P, Kaswati NMN. Time-dependent effect of chitosan nanoparticles as final irrigation on the apical sealing ability and push-out bond strength of root canal obturation.Int J of Dent 2020; 1(1): 1-6. DOI: 10.1155/2020/8887593

Singh H, Markan S, Kaur M, Gupta G. “Endodontic Sealers”: Current Concepts and Comparative Analysis. Dent - Open J. 2015;2(1):32–7. DOI: 10.17140/DOJ-2-107

Sonu KR, Girish TN, Ponnappa KC, Kishan K V, Thameem PK. “Comparative evaluation of dentinal penetration of three different endodontic sealers with and without smear layer removal” Scanning electron microscopic study. Saudi Endod J. 2016; 6(1): 16–20. DOI: 10.4103/1658-5984.171996

Jardine AP, Rosa RA da, Santini MF, Wagner M, Só MVR, Kuga MC, et al. The effect of final irrigation on the penetrability of an epoxy resin-based sealer into dentinal tubules: a confocal microscopy study. Clin Oral Investig. 2016;20(1):117–23. DOI: 10.1007/s00784-015-1474-8

Deviyanti S. Potensi larutan chitosan 0,2% sebagai alternatif bahan irigasi dalam perawatan saluran akar gigi. JITEKGI 2018; 14(1): 6-10. DOI: 10.32509/jitekgi.v14i1.642

Ayu FS, Trimurni A. Effect of irrigation with chitosan high molecule, sodium hypoclorite and EDTA to remove smear layer (in vitro). Dalam: Universitas Sumatera Utara, ed. Seminar Ilmiah Nasional 2015 dalam Rangka Dies Natalis USU 63, Medan, 2015: 48-55.

Ernani E, Abidin T, Indra I. Experimental comparative study and fracture resistance simulation with irrigation solution of 0.2% chitosan, 2.5% NaOCl and 17% EDTA. Dent J (Majalah Kedokteran Gigi) 2015; 48(3): 154-8. DOI: 10.20473/j.djmkg.v48.i3.p154-158

Suzuki S, Masuda Y, Morisaki H, Yamada Y, Kuwata H, Miyazaki T. The study of chitosan-citrate solution as a root canal irrigant: A preliminary report. J of Oral Hyg Health 2014; 2(4):1- 4. DOI: 10.4172/2332-0702.1000142

Naveed M, Phil L, Sohail M, Hasnat M, Muhammad M, Ashraf F, et al. International Journal of Biological Macromolecules Chitosan oligosaccharide (COS): An overview. Int J Biol Macromol. 2019; 1(1):827–43. DOI: 10.1016/j.ijbiomac.2019.01.192

Muanprasat C., Chatsudthipong V. Chitosan Olisaccharide: Biological Activities and Potential Therapeutic Applications. J. Pharm Thera. 2016:10:13. DOI: 10.1016/j.pharmthera.2016.10.013

Wang Y, Liu S, Dong Y. In vitro study of dentinal tubule penetration and filling quality of bioceramic sealer. PLOS ONE 2018; 13(2): 1-11. DOI: 10.1371/journal.pone.0192248

Al-Haddad A, Aziz ZACA. Bioceramic-based root canal sealers: A review. Int J of Biomaterials 2016; 1-10. DOI: 10.1155/2016/9753210

Kaur G, Reddy TVK, Ventakesh KV, Mahalakshmi. Effects of chitosan oligosaccharide and calcium hypochlorite on e. Faecalis dentinal biofilm and smear layer removal - SEM analysis. IJDR 2020; 31(04): 550-6. DOI: 10.4103/ijdr.IJDR_334_20

Pasricha SK, Makkar S, Gupta P. Pressure alteration techniques in endodontics- A review of literature. J of Clin and Diagnostic Research 2015; 9(3): 1-6. DOI: 10.7860/JCDR/2015/9249.5613

Saad AS. Physicochemical, cytotoxicity, and biological properties of calcium silicate-based root canal sealers: A literature review. Saudi Endodontic J 2020; 10(3): 173-80. DOI: 10.4103/sej.sej_160_19

Hosseini S, Kassaee M Z, Elahi S H, Bolhari B. A new nano-chitosan irrigant with superior smear layer removal and penetration. Nanochem Research 2016; 1(2): 150-6. DOI: 10.7508/NCR.2016.02.002

Yonathan, Ratnasari D. The effect of resin and bioceramic sealer on microleakage after root canal obturation. J of Indonesian Dent Association 2020; 3(2): 97-102. DOI: 10.32793/jida.v3i2.456

Camilleri, J. Characterization and hydration kinetics of tricalciumsilicate cementfor use as a dental biomaterial. Dent Material 2011;27(8): 836-844. DOI: 10.1016/j.dental.2011.04.010

Chellapandian K, Reddy TVK, Venkatesh V, Annapurani A. Bioceramic root canal sealers: A review. Int J Heal Sci, 2022; 6(S3), 5693–5706. DOI: 10.53730/ijhs.v6nS3.7214

Widjiastuti I, Rudyanto D, Yuanita T, Bramantoro T, Widodo WA. Cleaning efficacy of root canal irrigation with positive and negative pressure system. Iranian Endodontic J 2018; 13(3): 398- 402. DOI: 10.22037/iej.v13i3.20875

Hargraves Kenneth M, Berman Louis H, Rotstein I. Cohen’s pathways of the pulp. 11th ed, Canada: Elsevier. 2016: 132, 607-10

Marissa C, Usman M, Suprastiwi E, Erdiani A, Meidyawati R. Comparison of dentinal tubular penetration of three bioceramic sealers. Int J of Applied Pharmaceutics 2020; 12(Special Issue 2): 23-6. DOI: 10.22159/ijap.2020.v12s2.OP-05

DOI: https://doi.org/10.24198/pjd.vol34no3.39692