KARAKTERISASI SENYAWA ANTIMUTAGENIK DARI TUMBUHAN PAKAN PRIMATA, PUSPA (Schima wallichii Korth.)

Abstrak

The objective of this research is to find compound which has antimutagenic activity on primate-consumed plant, Puspa (Schima walichii Korth). In this research, antimutagenic activity tested on tested animal, mice, with micronucleus test and phytochemistry to know active compound that involved in this activity. Antimutagenic activity test was conducted by giving induction material, cyclophosphamide (50 mg/kg), to mice to induct the mutation or mutagenesis. The result showed that methanol extract at 1000 dan 2000 mg/kg, decreased micronucleus frequency significantly than the negative control. To trace the active compound that role in antimutagenic activity, metanol extract fracted and the effect of each fraction tested by the same method. The result indicated that etil acetate fraction at 450 and 900 mg/kg or water fraction at 700 and 1400 mg/kg decreased micronucleus frequency significantly than negative control. Its means that the both of fraction has antimutagenic activity, and based on the decreased number is knew that water fraction is stronger than etil acetate fraction activity. Then, water fraction fracted with  n-butanol until n­-butanol and water fraction obtained. Both of those fraction at 350 and 700 mg/kg doses give antimutagenic activity significantly, but water fraction has antimutagenic activity is stronger (percentage of micronukelus decrease frequency at 42,0% for n­-butanol 700 mg/kg, and 63,3% for water fraction 700 mg/kg). From etil acetate fraction was isolated one compound and so is the n-butanol fraction. To know its antimutagenic activity, the compound was tested with micronucleus method. But both of this structure compounds has not been determined yet. Thereby, this result gives information that primate-consumed plant potency to get new medicine for human health.