PENGARUH KAFEIN TERHADAP KUALITAS SPERMATOZOA IKAN MAS (Cyprinus carpio L) YANG DIIRADIASI ULTRAVIOLET

Abstrak

The research had been carried out to know about effect of caffeine on spermatozoa quality of common carp (Cyprinus carpio L) with UV - Irradiated by using experimental laboratory methods with a single factor of a complited randomized design (one group of control and five groups of treatment), had taken four times for each parameter. Semen samples diluted into MSS-Caffeine extender (1:1000) with variety of concentration (3mM, 4mM, 5mM, 6mM and 7mM) afterward irradiated during 5 minutes. Spermatozoa motility, viability and abnormality were counted from each 25 ml semen samples whereas for fertility used semen sample which had been diluted 100x.The result of this research indicated that caffeine caused increasing percentages of spermatozoa motility and fertility whereas  spermatozoa viablity and abnormality were not significantly to control group (Anova p<0.05). Duncan test indicated that variety of caffeine concentration giving was not significant for each treatment. Regression-correlation test indicated that caffeine giving  strongly correlation on spermatozoa motility and fertility. Thus, all concentration of caffeine can be used to increase spermatozoa quality which marked with increased spermatozoa motility and fertility where their viability and abnormality still on normal limit.