PENGARUH BERBAGAI KONSENTRASI DAN LAMA WAKTU PERENDAMAN GLIFOSAT TERHADAP MITOSIS DAN ABERASI KROMOSOM AKAR BAWANG BOMBAY (Allium cepa cv GLADALAN BROWN).

Abstrak

The research  was carried out to study the effect of various concentration  (0, 30, 50, 80 and 100 ppm )  and duration of soaking time (3, and 6 hours)  to mitotic index and chromosomal aberration  on  onion root (Allium cepa cv Gladalan Brown ). This research used experimental method with completely randomized design 2X5  factorial patterns. Experiment were repeated four times for every concentration and soaking time. The data was analyzed by using ANOVA test and The Newman- Keuls test. Permanent root squash preparation used Mc Clintock’s fixative solution and stained with aceto orcein. The result showed that various glyphosate  concentration and the duration of soaking time significantly influenced the  reduction of mitotic index value. The lowest mitotic index  on glyphosate concentration 100 ppm i.e. 10. 73% and 7.19% for the duration  of soaking time 3 and 6 hours. The highest mitotic index on  glyphosate concentration 0 ppm i.e.  37.71% and 32.76% for the duration  of soaking time 3 and 6 hours. The result also showed that the chromosomal aberration were increased significantly. The lowest  chromosomal aberration  obtained from the glyphosate concentration 0 ppm i.e. 2.55% and 2.96% for the duration of soaking time 3 and 6 hours. The highest chromosomal aberration obtained from  the glyphosate concentration 100 ppm i.e. 21.71% and 36.26% for the duration of soaking time 3 and 6 hours. The type of chromosomal aberration were abnormal prophase, stickiness, bridge, abnormal anaphase, clumping chromosome, c- metaphase,  change of nucleous  shape and size