Filtrate Culture of Trichoderma, Gliocladium, Penicilium and Aspergillus flavus as Inhibitor on Downy Mildew on Maize

Nur Ilmi, Sogandi Sogandi, Hikmahwati Hikmahwati

Abstract


Controlling downy mildew caused by Perenosclerospora philipinensis in maize plants is currently dependent on the use of synthetic pesticides; therefore, it is necessary to develop biocontrol agents as a sustainable alternative control. Filtrate cultures of biocontrol agent fungi consist of antibiotics, enzymes, and secondary metabolites that are antimicrobial in nature. We conducted the research from July to August 2024 at the Plant Disease Laboratory of the Department of Protection, Faculty of Agriculture, and the Biochemistry Laboratory of the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University. This study had four parts: (1) identifying the morphology of biocontrol agent isolates and pathogenic fungi; (2) measuring growth diameter of antagonist fungi;(3) testing filtrate cultures for their ability to inhibit pathogens; and (4) testing filtrate cultures for their ability in vigor of sprouting on corn seeds. Based on morphological identification, the four biocontrol agent isolates are Trichoderma, Gliocladium, Penicillium, and Aspergillus flavus, with the best growth diameter on the Trichoderma, which is 8.5 cm on seven days. The filtrate cultures performed the best in the inhibition test on P. philipinensis, exhibiting spore damage percentages of 70-74%. The vigor test performed best on    Trichoderma and Aspergillus flavus, with growth potential (GP) of 100% and 63%, respectively. Filtrate culture with biological agents has good potential for P. philipinensis spore inhibition and corn seed germination.

Keywords


Peronosclerospora phipinenesis, Vigor, Konidia, Benih

References


Abdelaziz, AM, Kalaba MH, Hashem AH, Sharaf M. H, & Attia MS. (2022). Biostimulation of tomato growth and biocontrol of Fusarium wilt disease using certain endophytic fungi. Botanical Studies, 63(1). https://doi.org/10. 1186/ s40529-022-00364-7

Adhi, S. R., Widiantini, F., & Yulia, E. (2019). Metode inokulasi buatan untuk menguji infeksi Peronosclerospora maydis penyebab penyakit bulai tanaman jagung. Jurnal Agro, 6(1), 77–85. https://doi.org/10.15575/4409

Amin, N., Daha, L., Nasruddin, A., Junaed, M., & Iqbal, A. (2013). The use of endophytic fungi as biopesticide against downy mildew Peronosclerospora spp. On maize. Natural and Applied Science. www.savap.org.pkwww. journals.savap.org.pk

Ammar, H. A. M., Awny, N. M., & Fahmy, H. M. (2017). Influence of environmental conditions of atoxigenic Aspergillus flavus HFB1 on biocontrol of patulin produced by a novel apple contaminant isolate, A. terreusHAP1, in vivo and in vitro. Biocatalysis and Agricultural Biotechnology, 12(August), 36–44. https://doi.org/10.1016/j.bcab.2017.08.011

Anugrah, F. M., & Widiantini, F. (2018). The Effect Of Metalaxyl, Fenamidone, And Dimetomorf Fungicide Towards Conidia Peronosclerospora spp. Isolated From Klaten). Jurnal Penelitian Saintek, 3(1), 21–31.

Behiry, S., Soliman, S. A., Massoud, M. A., Abdelbary, M., Kordy, A. M., Abdelkhalek, A., & Heflish, A. (2023). Trichoderma pubescens Elicit Induced Systemic Resistance in Tomato Challenged by Rhizoctonia solani. Journal of Fungi, 9(2), 167. https://doi.org/10.3390/jof9020167

Drott, M. T., A, H. S., & Milgroom, M. G. (2019). crossm Fitness Cost of Aflatoxin Production in Aspergillus flavus When. Ecological and Evolutionary Science, 10(1), 1–13.

Efri, Prasetyo J., & Suharjo R. (2009). Skrining Dan Uji Antagonis Mejamur Trichoderma harzianum yang Mampu bertahan Di Filosfer Tanaman Jagung. HPT Tropika, 9(2), 121–129. https://doi.org/10.23960/j.hptt.29121-129

Espeso, E. A., Villarino, M., Carreras, M., Alonso-Guirado, L., Alonso, J. M., Melgarejo, P., & Larena, I. (2019). Altered nitrogen metabolism in biocontrol strains of Penicillium rubens. Fungal Genetics and Biology, 132(April), 103263. https://doi.org/10.1016/j.fgb.2019.103263

Ginting, C., Prasetyo, J., Dirmawati, S. R., Ivayani, ., Timotiwu, P. B., Maryono, T., Widyastuti, ., Chafisa, D. I. R., Asyifa, A., Setyowati, E., & Pasaribu, A. H. Z. (2020). Identification of Maize Downy Mildew Pathogen in Lampung and the Effects of Varieties and Metalaxyl on Disease Incidence. Annual Research & Review in Biology, 23–35. https://doi.org/10.9734/arrb/2020/v35i730244

Gowda, P. S. B., & Bhat, S. S. (1988). In vitro effects of metalaxyl on Peronosclerospora sorghi and sorghum callus. Transactions of the British Mycological Society, 91(3), 403–408. https://doi.org/10.1016/S0007-1536(88)80115-3

Hikmahwati, Fitrianti, & Karim, H. A. (2021). Rhizosphere Mushrooms : Antagonistic Exploration of Rhizospere Mushrooms in Shallot ( Allium ascolonicum L ) Specific Location of Enrekang Regency. International Journal of Scientific Research in Science and Technology Print, 8(6), 428–433. https://doi.org/10.32628/IJSRST218520

Hikmahwati, Kuswinanti, T., & Melina. (2011). Karakterisasi Morofologi Peronosclerospora spp. Penyebab Penyakit Bulai pada tanaman Jagung di Beberapa Daerah di Indonesia. Fitomedika, 7(3), 159–161.

Hikmahwati, Suharman, & Fitrianti. (2022). Rhizospheric Fungus : Morphological Characterization Of Rhizosphere Flower On Onion Plant In Enrekang District. Juatika Jurnal Agronomi Tanaman Tropika, 4(1).

Houbraken, J., Frisvad, J. C., & Samson, R. A. (2011). Taxonomy of Penicillium section Citrina. Studies in Mycology, 70, 53–138. https://doi.org/10.3114/sim.2011.70.02

Howell, C. R. (2003). Mechanisms employed by Trichoderma species in the biological control of plant diseases: The history and evolution of current concepts. Plant Disease, 87(1), 4–10. https://doi.org/10.1094/PDIS.2003.87.1.4

Hunter, H. L. B. and B. B. (2006). llustrated Genera of Imperfect Fungi (fourth) . The American Phytopatological Society.

Jatnika, W., Abadi, A., & Aini AQ. (2013). Pengaruh aplikasi bacillus sp. Dan Pseudomonas sp. Terhadap perkembangan penyakit bulai yang disebabkan oleh jamur patogen Peronosclerospora maydis pada tanaman jagung. HPT, 1(4), 19–29.

Kamble, M. V., Joshi, S. M., Hadimani, S., & Jogaiah, S. (2021a). Biopriming with rhizosphere Trichoderma harzianum elicit protection against grapevine downy mildew disease by triggering histopathological and biochemical defense responses. Rhizosphere, 19. https://doi.org/10.1016/j.rhisph.2021.100398

Keinath, A. P., & de Figueiredo Silva, F. (2022). Economic impacts of reduced fungicide efficacy against downy mildew on slicing cucumber. Crop Protection, 155, 105934. https://doi.org/10.1016/J.CROPRO.2022.105934

Khan, I., Qayyum, S., Ahmed, S., Haleem, K. S., Mujaddad-ur-Rehman, M.-R., Liu, G.-L., & Chi, Z.-M. (2017). Isolation and Characterization of Medicinally Important Marine Penicillium Isolates. Pakistan Journal of Zoology, 49(2), 435–441. https://doi.org /10.17582/journal.pjz/2017.49.2.435.441

Larekeng, G. dan S. H. (2020). Karakterisasi Cendawan Rhizosfer Pada Tegakan Mahoni Di Hutan Pendidikan Universitas Hasanuddin Characterization of Rhizosphere Fungi Mahogany at Experimental Forest Hasanuddin University. Galung Tropika, 9(3), 276–285.

Mirsam, H., Kalqutny, S. H., Suriani, Aqil, M., Azrai, M., Pakki, S., Muis, A., Djaenuddin, N., Rauf, A. W., & Muslimin. (2021a). Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn. Heliyon, 7(9). https://doi.org/10.1016/j.heliyon.2021.e07926

Mirsam, H., Suriani, Muis, A., Pakki, S., Nonci, N., Kurniawati, S., Purwanto, O., & Azrai, M. (2024). Plant growth-promoting indigenous fungi from maize as biological control agents of Fusarium verticillioides and its role to improve maize seed germination. International Conference on Food and Agricultural Sciences, 1–10. https://doi.org/https://doi.org/10.1063/ 5.0183937

Muis, A., Pabendon, M. B., Nonci, N., Wahyu Purbowasito Setyo Waskito, dan, Penelitian Tanaman Serealia Jl Ratulangi, B., & Selatan, S. (2013). Keragaman Genetik Peronosclerospora maydis Penyebab Bulai pada Jagung Berdasarkan Analisis Marka SSR. Penelitian Pertanian Tanaman Pangan, 32(3), 139–147. www.powermarker.net

Nandhini, M., Rajini, S. B., Udayashankar, A. C., Niranjana, S. R., Lund, O. S., Shetty, H. S., & Prakash, H. S. (2018). Diversity, plant growth promoting and downy mildew disease suppression potential of cultivable endophytic fungal communities associated with pearl millet. Biological Control, 127, 127–138. https://doi.org/10.1016/j.biocontrol.2018.08.019

Nguyen, P. A., Strub, C., Fontana, A., & Schorr-Galindo, S. (2017). Crop molds and mycotoxins: Alternative management using biocontrol. In Biological Control (Vol. 104, pp. 10–27). Academic Press Inc. https://doi.org/ 10.1016/j.biocontrol.2016.10.004

Nofal, A. M., El-Rahman, M. A., Abdelghany, T. M., & Abd El-Mongy, M. (2021). Mycoparasitic nature of Egyptian Trichoderma isolates and their impact on suppression Fusarium wilt of tomato. Egyptian Journal of Biological Pest Control, 31(1), 1–8. https://doi.org/10.1186/s41938-021-00450-1

Nofal, A. M., Hamouda, R. A., Rizk, A., El-Rahman, M. A., Takla, A. K., Galal, H., Alqahtani, M. D., Alharbi, B. M., Elkelish, A., & Shaheen, S. (2024). Polyphenols-Rich Extract of Calotropis procera Alone and in Combination with Trichoderma Culture Filtrate for Biocontrol of Cantaloupe Wilt and Root Rot Fungi. Molecules, 29(1). https://doi.org/10.3390/molecules29010139

Nurulita, Y., Yuharmen, Y., Nenci, N., Mellani, A. O., & Nugroho, T. T. (2020). Metabolit Sekunder Sekresi Jamur Penicillium spp. Isolat Tanah Gambut Riau sebagai Antijamur Candida albicans. Chimica et Natura Acta, 8(3), 133. https://doi.org/10.24198/cna.v8.n3.32452

Pereira-Dias, L., Oliveira-Pinto, P. R., Fernandes, J. O., Regalado, L., Mendes, R., Teixeira, C., Mariz-Ponte, N., Gomes, P., & Santos, C. (2023). Peptaibiotics: Harnessing the potential of microbial secondary metabolites for mitigation of plant pathogens. Biotechnology Advances, 68(August). https://doi.org/10.1016/j.biotechadv.2023.108223

Prasetyo, J., . S., Ginting, C., & Permatasari, Y. C. (2019). The Effectiveness of Trichoderma spp. against Downy Mildew Disease of Corn. Annual Research & Review in Biology, 1–10. https://doi.org/10.9734/arrb/2019/v31i630068

Prasetyo, J., Ginting, C., Akin, H. M., Suharjo, R., Niswati, A., Afandi, A., Adiwijaya, R., Sudiono, & Nurdin, M. (2021). The effect of biological agent and botanical fungicides on maize downy mildew. Biodiversitas, 22(4), 1652–1657. https://doi.org/10.13057/biodiv/d220409

Pudjiwati, E. H., Kuswanto, Basuki, N., & Noor Sugiharto, A. (2013). Path analysis of some leaf characters related to downy mildew resistance in maize. Agrivita, 35(2), 167–173. https://doi.org/10.17503/agrivita-2013-35-2-p1 67-173

Richa, Saxena, R., Srivastava, M., & Singh, H. V. (2025). Influence of native microbial biocontrol agent on radial growth of Fusarium oxysporum f. sp. ciceri isolates. Sustainability, Agri., Food and Environmental Research, 13, 1–18.

Rini Widiati, B., Herwati, A., & Sofyan. (2022). Identification of Rhizosphere Fungi in Corn (Zea Mays L.) and Test of the Effectiveness of Trichoderma sp. Propagation Media. Jurnal Galung Tropika, 11(3), 262–274. https://doi.org/10.31850/jgt.v11i3.1000

Silaban, I. C., Luqman Qurata Aini, & Muhammad Akhid Syib’li. (2015). pengujian konsorsium mikroba antagonis untuk mengendalikan jamur sclerotiumrolfsii penyebab penyakit rebahsemai pada kedelai(Glycine MaxL.). Jurnal HPT, 3(2), 100–107.

Soares Guimarães, L. H., Segura, F. R., Tonani, L., von-Zeska-Kress, M. R., Rodrigues, J. L., Calixto, L. A., Silva, F. F., & Batista, B. L. (2019). Arsenic volatilization by Aspergillus sp. and Penicillium sp. isolated from rice rhizosphere as a promising eco-safe tool for arsenic mitigation. Journal of Environmental Management, 237(February), 170–179. https://doi.org/10.1016/j.jenvman.2019.02.060

Sonhaji, Y. M., Surahman, M., Ilyas, S., Giyanto, dan, Sumber Agung, D., Plosoklaten, K., & Timur, J. (2013). Seed Treatment Improved Seed Quality, Seed Production and Controlled Downey Mildew Disease on Sweet Corn. J. Agron. Indonesia, 41(3), 242–248.

Syamsulhadi, M., Ramadhan, V. T., & Widjayanti, T. (2023). Pertumbuhan Jamur Beauveria bassiana Pada Beberapa Tingkat Keasaman Media Dan Suhu Penyimpanan Serta Efektivitasnya Terhadap Hama Spodoptera litura. Jurnal Hama Dan Penyakit Tumbuhan, 11(1), 28–41. https://doi.org/10.21776/ub.jurna lhpt.2023.011.1.4

Yassin, M. T., Mostafa, A. A. F., & Al-Askar, A. A. (2021). In vitro antagonistic activity of Trichoderma harzianum and T. viride strains compared to carbendazim fungicide against the fungal phytopathogens of Sorghum bicolor (L.) Moench. Egyptian Journal of Biological Pest Control, 31(1). https://doi.org/10.1186/s41938-021-00463-w

Zhu, G. Y., Shi, X. C., Herrera-Balandrano, D. D., Wang, S. Y., & Laborda, P. (2022). Non-aflatoxigenic kojic acid-producing Aspergillus flavus NJC04 reduces the symptoms of Sclerotinia sclerotiorum in soybean. Biological Control, 176(June). https://doi.org/10.1016/j.biocontrol.2022.105064




DOI: https://doi.org/10.24198/cropsaver.v7i2.57771

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