The Ability of Three Species of Yeast in Inhibiting the in vitro Growth of Sclerotium rolfsii Sacc., the cause of damping off on soybean plants (Glycine max L.)

Sri Hartati, Cahya Setiani, Rika Meliansyah, Endah Yulia, Tri Mayanti

Abstract


Damping off disease caused by Sclerotium rolfsii is one of the important diseases of soybean plants. Biocontrol is considered as more environmentally friendly control method. Yeast is one of the biocontrol agents that can be used to control plant pathogens. This study was objected to test the potential of three species of yeast in inhibiting the growth of S. rolfsii. The experiment was arranged in the completely randomized design. The treatments were dual culture of the pathogen vs the yeasts on PDA and double dishes of S. rolfsii against three yeast isolates i.e. Rhodotorula minuta Dmg 16 BEP, Candida tropicalis Lm 13 BE, Aureobasidium pullulans Dmg 11 DEP, and a control. The results of the dual culture antagonism test showed that the three tested yeasts could inhibit the colony diameter of S. rolfsii by 23.30% – 40.00%, and the sclerotia formation by 46,33% – 98,05%. The results of the double dishes antagonism test showed that the three tested yeast isolates could inhibit the colony diameter of S. rolfsii by 19.60% – 28.20% and could inhibit 100% of sclerotia formation. The treatment of A. pullulans Dmg 11 DEP produced the highest inhibition in both the dual culture and double dishes antagonism tests.

Keywords


Aureobasidium pullulans, Biocontrol, Double dish system, Dual culture, Sclerotia

References


Adhi, SR, & Suganda T. 2020. Potensi jamur rizosfer bawang merah dalam menekan Fusarium oxysporum f.sp. cepae penyebab penyakit busuk umbi bawang merah. Jurnal Kultivasi. 19(1): 1015-1022. DOI:10.24198/kultivasi.v19i1.22877

Agirman, B, & Erten H. 2020. Biocontrol ability and action mechanisms of Aureobasidium pullulans GE17 and Meyerozyma guilliermondii KL3 against Penicillium digitatum DSM2750 and Penicillium expansum DSM62841 causing postharvest diseases. Yeast. 37(9-10): 437-448. DOI: https://doi.org/10.1002/yea.3501

Amanupunyo, HR, Tahitu NE, & Tuhumury GN. 2021. Efektivitas Limbah Cengkih dalam Menekan Perkembangan In Vitro Sclerotium rolfsii, Jamur Penyebab Damping Off Kacang Tanah. Jurnal Budidaya Pertanian, 17(1): 36-42.

Arrarte, E, Garmendia G, Rossini C, Wisniewski M, & Vero S. 2017. Volatile organic compounds produced by Antarctic strains of Candida sake play a role in the control of postharvest pathogens of apples. Biological Control. 109: 14–20. DOI: https://doi.org/10.1016/j.biocontrol.2017.03.002

[BSIP] Balai Pengujian Standar Instrumen Tanaman Aneka Kacang Badan Standardisasi Instrumen Pertanian. 2023. Berita BSIP Aneka Kacang Balai Pengujian Standar Instrumen Tanaman Aneka Kacang [internet]. [diacu 2024 Juli 8]. Tersedia dari https://anekakacang.bsip. pertanian.go.id/berita/bsip-aneka-kacang-siap-dukung-peningkatan-produktivitas-kedelai-di-jawa-timur#

Cai, T, Shi P, Zhang S, Xiang W, Liu J, Lin Z, & Tang J. 2023. Inhibition of perilla frutescens essential oil on pellicle formation of Candida tropicalis and Pichia kluyveri and its effect on volatile compounds in sichuan pickles. Foods. 12(8): 1593. DOI: 10.3390/foods12081593

Chaurasia, B, Pandey A, Palni LMS, Trivedi P, Kumar PB, & Colvin N. 2005. Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro. Microbiological research. 160(1): 75-81.

Dalilla, CR, Mauricio BF, Simone CB, Silvia B, & Sergio FP. 2015. Antimicrobial activity of volatile organic compounds and their effect on lipid peroxidation and electrolyte loss in Colletotrichum gloeosporioides and Colletotrichum acutatum mycelia. African Journal of Microbiology Research. 9 (23): 1527–1535. DOI: https://doi.org/10.5897/ajmr2015.7425

Díaz, MA , Pereyra MM, Picón-Montenegro E, Meinhardt F, & Dib JR. 2020. Review: Killer yeasts for the biological control of postharvest fungal crop diseases. Microorganisms. 8(1680): 1-14. DOI: 10.3390/microorganisms8111680

Di Francesco, A, Zajc J, Gunde-Cimerman N, Aprea E, Gasperi F, Placì N, Caruso F, & Baraldi E. 2020. Bioactivity of volatile organic compounds by Aureobasidium species against gray mold of tomato and table grape. World Journal of Microbiology and Biotechnology. 36(11): 171. DOI: 10.1007/s11274-020-02947-7.

[DJTP] Direktorat Jenderal Tanaman Pangan. 2024. Laporan Tahun 2023 Direktorat Jenderal Tanaman Pangan [internet]. [diacu 2024 Juli 8]. Tersedia dari https://tanamanpangan.pertanian. go.id/assets/front/uploads/document/LAPORAN%20TAHUNAN%202023

Don, SMY, Schmidtke LM, Gambetta JM, & Steel CC. 2021. Volatile organic compounds produced by Aureobasidium pullulans induce electrolyte loss and oxidative stress in Botrytis cinerea and Alternaria alternata. Research in Microbiology. 172(1): 103788. DOI: 10.1016/j.resmic.2020.10.003

Dwiastuti, ME, Soesanto L, Aji TG, Devy NF, & Hardiyanto. 2021. Biological control strategy for postharvest diseases of citrus, apples, grapes and strawberries fruits and application in Indonesia. Egyptian Journal of Biological Pest Control. 31: 141. DOI: 10.1186/s41938-021-00488-1

Ferraz, LP, da Cunha T, da Silva AC, & Kupper KC. 2016. Biocontrol ability and putative mode of action of yeasts against Geotrichum citri aurantii in citrus fruit. Microbiological Research. 4(12): 1-28. DOI: 10.1016/j.micres.2016.04.012

Freimoser, FM, Rueda-Mejia MP, Tilocca B, & Migheli Q. 2019. Biocontrol yeasts: mechanisms and applications. World Journal of Microbiology and Biotechnology. 35: 154. DOIi: 10.1007/s11274-019-2728-4

Haggag, WM, & HALA Mohamed. 2007. Biotechnological Aspects of Microorganisms Used in Plant Biological Control. American Eurasian journal of sustainable agriculture. 1(1): 7-12.

Hartati, S, Utari ED, Rasiska S, & Istifadah N. 2022. Capability of Three Yeast Species in Suppressing Green Mold (Penicillium digitatum) on Siam Citrus Fruit (Citrus nobilis). Cropsaver-Journal of Plant Protection. 5(2), 61-70.

Hendriadi A, Sulistiyorini S, & Devilana MR. 2021. Pesticides residues in fresh food of plant origin: case study in Indonesia. Agrivita Journal of Agricultural Science. 43:285–299. DOI: 10.17503/agrivita.v43i2.2570

Huang, R, Li GQ, Zhang J, Yang L, Che HJ, Jiang DH, & Huang HC. 2011. Disease control and pest management control of postharvest botrytis fruit rot of strawberry by volatile organic compounds of Candida intermedia. Phytopathology. 101, 859-869.

Idris, H, Agustien A, & Mansyurdin M. 2023. Pengendalian Athelia rolfsii Penyebab Busuk Pangkal Batang Pada Kacang Tanah Arachis hypogea. L dengan Fungisida Nabati Dan Agensia Hayati. Jurnal Agrosains dan Teknologi. 8(2): 87-93.

Intan, RMT, Cholil A, & Sulistyowati L. 2014. Potensi antagonis jamur endofit dan khamir pada tanaman pisang (Musa accumunata) terhadap jamur Mycosphaerella musicola penyebab penyakit bercak kuning sigatoka. Jurnal HPT (Hama Penyakit Tumbuhan). 2(4): 110-118.

Iqbal, M, Jamshaid M, Zahid MA, Andreasson E, Vetukuri RR, & Stenberg JA. 2021. Biological control of strawberry crown rot, root rot and grey mould by the beneficial fungus Aureobasidium pullulans. BioControl. 66(4): 535-545.

Janisiewicz, WJ, & Korsten L. 2002. Biological control of postharvest diseases of fruits. Annual Review of Phytopathology. 40:411–41. DOI: 10.1146/annurev.phyto.40.120401.130158

Khoerunnisa, R, & Handayani THW. 2018. Mini Tart Kedelai sebagai Pengembangan Produk Patiseri Berbahan Substitusi Tepung Kedelai Lokal. Prosiding Pendidikan Teknik Boga Busana. 13(1): 1-5.

Kowalska, J, Krzymińska J, & Tyburski J. 2022. Yeasts as a potential biological agent in plant disease protection and yield improvement a short review. Agriculture (Switzerland): 12(9). https://doi.org/10.3390/agriculture12091404

Mori, M, Aoyama M, Doi S, Kanetoshi A, & Hayashi T. 1997. Antifungal activity of bark extracts of deciduous trees. European Journal of Wood and Wood Products. 55(2-4): 130-132.

Nasahi, C, Yusuf AR, Hartati S, Kurniadie D, & Subekti-Putri SN. 2023. Yeast potential in controlling Aspergillus sp. causing fruit rot disease in dekopon oranges (Citrus reticulata ‘Shiranui’). Research on Crops. 24(2): 407-415. DOI: 10.31830/2348-7542.2023.ROC-922

Oufensou, S, Hassan UIZ, Balmas V, Jaoua S, & Migheli Q. 2023. Perfume guns: Potential of yeast volatile organic compounds in the biological control of mycotoxin-producing fungi. Toxins. 15(1): 45.

Pinto, C, Custódio V, Nunes M, Songy A, Rabenoelina F, Courteaux B, & Fontaine F. 2018. Understand the potential role of Aureobasidium pullulans, a resident microorganism from grapevine, to prevent the infection caused by Diplodia seriata. Frontiers in Microbiology. 9: 3047. DOI: 10.3389/fmicb.2018.03047

Podgórska-Kryszczuk, I. 2023. Biological control of Aspergillus flavus by the yeast Aureobasidium pullulans in vitro and on tomato fruit. Plants. 12(2): 236. DOI: 10.3390/plants12020236

Prasongsuk, S, Lotrakul P, Ali I, Bankeeree W, & H Punnapayak. 2018. The current status of Aureobasidium pullulans in biotechnology. Folia Microbiologica. 63: 129-140. DOI: 10.1007/s12223-017-0561-4

Raspor, P, Miklič-Milek D, Avbelj M, & Čadež N. 2010. Biocontrol of Grey Mould Disease on Grape Caused by Botrytis cinerea with Autochthonous Wine Yeasts. Food Technology & Biotechnology. 48(3).

Ruiz-Moyano, S, Hernández A, Galvan AI, Córdoba MG, Casquete R, Serradilla MJ, & Martín A. 2020. Selection and application of antifungal VOCs-producing yeasts as biocontrol agents of grey mould in fruits. Food microbiology. 92: 1-6.

Semangun, H. 2004. Diseases of food crops in Indonesia. Gadjah Mada University Press, Yogyakarta. 850p.

Setiawan, A, Sastrahidayat IR, & Muhibuddin A. 2014. Upaya penekanan serangan penyakit rebah semai (Sclerotium roflsii) pada tanaman kedelai (Glycine max L.) dengan mikoriza yang diperbanyak dengan inang perantara tanaman kacang tanah. Jurnal HPT (Hama Penyakit Tumbuhan). 2(4): 36-43.

Setiawan, W, Wiyono S, Tondok ET, Kanti A, & Sudiana IM. 2020. In vitro Study of Action Mode of Rhodotorula minuta Dmg 16 BEP as Biocontrol Agents on Alternaria solani. Jurnal Perlindungan Tanaman Indonesia. 24: 28-33.

Sriram, S, & Poornachanddra SR. 2013. Biological control of postharvest mango fruit rot caused by Colletotrichum gloeosporioides and Diplodia natalensi with Candida tropicalis and Alcaligenes feacalis. Indian Phytopathology. 66(4): 375-380.

Tongsri, V, Sanosomneng K, Umrung S, & Montri N. 2022. Antagonistic activity of Candida utilis SCKU1 yeast against crown rot disease of 'Hom Thong' banana (Musa acuminata, AAA group). International Journal of Agricultural Technology 18(4): 1867-1868

Urbina, CT, Prieto VG, Lopez CG, Albores FV, Reyes DB, Muniz CA, & Barrios DO. 2016. Purification and characterization of b-1,3- glucanase from Candida oleophila for the biocontrol of Penicillium expansum. Research & Reviews: Journal of Botanical Sciences. 5 (1): 38-45.

Wang, P, Jia SL, Liu GI, Chi Z, & Chi ZM. 2022. Aureobasidium spp. and their applications in biotechnology. Process Biochemistry. 116: 72-83.

Yalage Don, SM, Schmidtke LM, Gambetta JM, & Steel CC. 2021. Volatile organic compounds produced by Aureobasidium pullulans induce electrolyte loss and oxidative stress in Botrytis cinerea and Alternaria alternata. Research in Microbiology. 172(1). https://doi.org/10.1016/j.resmic.2020.10.003

Zhang X, Li Y, T, u M X, Guo J, Zhang C, Feng Z, Peng, Li Z, Xing K, & Qin S. 2021. Antifungal effect of volatile organic compounds produced by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 on oxidative stress and mitochondrial dysfunction of ceratocystis fimbriata. Pesticide Biochemistry and Physiology. 173. https://doi.org/10.1016/j.pestbp.2021.104777

Zhou, J, Xiong K, Yang Y, Ye X, Liu J, & F Li. 2015. Deleterious effects of benomyl and carbendazim on human placental trophoblast cells. Reproductive Toxicology, 51, 64–71. https://doi.org/10.1016/j.reprotox.2014.12.008




DOI: https://doi.org/10.24198/cropsaver.v7i2.58059

Refbacks

  • There are currently no refbacks.


Copyright (c) 2024 CROPSAVER - Journal of Plant Protection

2019 © Publisher by Department of Plant Pests and Diseases, Universitas Padjadjaran

In Collaboration With

Centre for Product Development and Partnership Study (Puspromit)

Indonesian Entomological Society (PEI) and

Indonesian Phytopathological Society (PFI)

Chapter Bandung


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.