Consortium of Bacillus sp. BTH22 and Trichoderma sp. to Control Bacterial Leaf Blight Disease in Rice Plants
Abstract
Rice is a major food commodity in Indonesia that is currently experiencing a decline in production due to several factors, one of which is bacterial leaf blight (BLB) caused by the bacterium Xanthomonas sp. The use of chemicals in controlling this disease has many limitations, so alternatives are needed, such as antagonistic microbes. This study aims to evaluate the effectiveness of Bacillus sp. BTH22 and Trichoderma sp. individually and in consortium in suppressing BLB disease in rice plants in vitro and in vivo. The study was conducted at the Plant Health Laboratory of Universitas Pembangunan Nasional “Veteran” Jawa Timur and Green House of Kebun Bibit Wonorejo from March to June 2025. The methods used included in vitro diffusion well test and in vivo disease intensity observation with a completely randomized design consisting of 5 treatments, namely control and spraying of antagonistic microbes Bacillus sp. BTH22 and Trichoderma sp. alone or in consortium with 4 replicates. Data analysis used analysis of variance (ANOVA) and post-hoc HSD tests at a 5% significance level. The results show that the treatment with the Bacillus sp. BTH22 and Trichoderma sp. consortium was the most effective, with an inhibition zone of 13 mm, which is classified as strong, and the lowest disease intensity of 30.02% at 55 HST with a suppression rate of 69.98%. These results indicate that the consortium of the two antagonistic microorganisms has high potential as an effective and sustainable biological control agent for BLB based on the observed parameters of inhibition zone and disease attack intensity.
Keywords
Full Text:
PDF (Bahasa Indonesia)References
Arsi, Lailaturrahmi, Suparman SHK, Hamidson H, Pujiastuti Y, Gunawan B, Pratama R, & Umayah A. 2022. Inventarisasi spesies dan intensitas serangan hama tanaman terung (Solanum melongena L.) pada dua sistem kultur teknis di daerah Kabupaten Agam, Sumatera Barat. Jurnal Agrikultura, 33(2): 126-137. https://jurnal.unpad.ac.id/agrikultura/article/view/40249/18200.
Badan Pusat Statistik. 2024. Luas Panen, produksi, dan produktivitas padi menurut provinsi, 2024. Badan Pusat Statistik.
Hersanti A. Arisaputri, & Istifadah N. 2024. Uji kitosan nano dan silika nano untuk menekan pertumbuhan Rhizoctonia solani dan penyakit rebah semai padi. Jurnal Agrikultura, 35(1): 30–38. https://doi.org/10.24198/agrikultura.v35i1.50758
Istiqomah, Kusumawati DE, & Serdani AD. 2022. Inovasi aplikasi asap cair dan agens hayati sebagai upaya pengendalian serangan hama dan penyakit pada padi (Oryza sativa L.). Jurnal Buana Sains, 22(1): 1–10. https://doi.org/10.33366/bs.v22i1.3083
Masnilah R, Wahyuni WSNSD, Majid A, Addy HS, & Wafa A. 2020. Insidensi dan keparahan penyakit penting tanaman padi di kabupaten Jember. Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science), 18(1): 1–12. https://doi.org/10.32528/agritrop.v18i1.3103
Mendrofa YT, & Lase NK. 2025. Peranan bakteri Bacillus sp. Sebagai agen biofertilizer dalam meningkatkan kesuburan tanah dan produktivitas tanaman : kajian literatur. Hidroponik: Jurnal Ilmu Pertanian Dan Teknologi Dalam Ilmu Tanaman, 2(1): 79-85. https://doi.org/10.62951/hidroponik.v2i1.227
Nikko, Ramdan EP, Risnawati, & Sugeru H. 2023. Suppression of Xanthomonas oryzae pv. oryzae infection in rice seeds: investigating the optimal temperature and packaging conditions for enhanced pathogen control and seed quality. Jurnal Ilmiah Pertanian, 20(2): 109-117. https://doi.org/10.31849/jip.v20i2.13205
Putri Doo, SR, Meitiniarti I, Kasmiyati S, & Kristiani EBE. 2023. Trichoderma spp., si jamur multi fungsi. Tropical Microbiome Journal, 1(1), 73–89. https://ejournal.uksw.edu/jtm/article/view/10659/2593/42428
Putri UD, & Anhar A. 2024. Trichoderma sp: solusi ramah lingkungan untuk pengendalian patogen dan peningkatan pertumbuhan tanaman. Prosiding Seminar Nasional Biologi, 4(1): 222-229. https://doi.org/10.24036/prosemnasbio/vol4/939
Sayekti NA, Purnawati A, & Lestari SR. 2025. The Inhibitory Ability of Endophytic Bacteria Bacillus sp. BTH-22 and BTH-31a to the Growth of Xanthomonas oryzae in Vitro. Nusantara Science and Technology Proceedings, 2025(49): 108-111. https://doi.org/10.11594/nstp.2025.4914
Senol, M., Nadaroglu, H., Dikbas, N., & Kotan, R. (2014). Purification of chitinase enzymes from Bacillus subtilis bacteria TV-125, investigation of kinetic properties and antifungal activity against Fusarium culmorum. Annals of Clinical Microbiology and Antimicrobials, 13(35), 1-7.
Setyowati L, Purnawati A, Mujoko T, & Mukaromah F. (2024). Secondary metabolites of Bacillus sp. as antifungal of seed-borne pathogen fungi on maize seed using blotter test method. BIOEDUSCIENCE, 8(1): 116–125. https://doi.org/10.22236/jbes/12588
Wally P, Marwah AS, & Warang AF. 2022. Efektivitas Ekstrak Myristica fragrans Houtt Terhadap Bakteri Patogen Pseudomonas aeruginosa dan Methicilin Resistensi Staphylococcus aureus. Jurnal Biotek, 10(2): 224-239. https://doi.org/10.24252/jb.v10i2.31930
Wardahni H, Mujoko T, & Purnawati A. 2022. Potensi Metabolit Sekunder Trichoderma harzianum terhadap Xanthomonas campestris pv. vesicatoria Secara In Vitro. Agrohita, 7(3): 539-546. DOI:10.31604/jap.v7i3.7046
Yanti Y, Nurbailis, Dwipa i, & Suhendra D. 2025. Potensi Bacillus spp. sebagai agens biokontrol pengendali penyakit layu fusarium (Fusarium oxysporum f. sp. cepae) dan pengaruhnya terhadap pertumbuhan bawang merah (Allium ascalonicum L.). Jurnal Agrikultura, 36(1): 105-114. https://jurnal.unpad.ac.id/agrikultura/article/view/55165/25489
DOI: https://doi.org/10.24198/cropsaver.v8i2.65958
Refbacks
- There are currently no refbacks.
Copyright (c) 2026 CROPSAVER - Journal of Plant Protection
2019 © Publisher by Department of Plant Pests and Diseases, Universitas Padjadjaran
In Collaboration With
Centre for Product Development and Partnership Study (Puspromit)
Indonesian Entomological Society (PEI) and
Indonesian Phytopathological Society (PFI)
Chapter Bandung
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
