Biodegradation Potential of Streptomyces sp. Against propineb-Based 70% Fungicide Under In Vitro Conditions
Abstract
The intensive use of propineb-based fungicides in agriculture can negatively affect the environment due to toxic and persistent residues in soil. An environmentally friendly approach to reduce these impacts is bioremediation using microorganisms such as Streptomyces sp. This study aimed to examine the tolerance and biodegradation potential of Streptomyces sp. toward 70% propineb fungicide under in vitro conditions. The research was conducted at the Plant Health 1 Laboratory, Universitas Pembangunan Nasional “Veteran” East Java, from June to August 2025. The experiments were carried out in two stages: (1) a tolerance assay on Glucose Nutrient Agar containing propineb at 0; 0,50; 1,00; 1,50; and 2,00 g/L, and (2) a degradation assay using a dual-culture method with Colletotrichum sp. as a bioindicator. Observation variables included Total Plate Count (TPC) of Streptomyces sp., colony diameter of Colletotrichum sp., and the relative inhibition level (THR). Results showed that Streptomyces sp. was able to grow at all fungicide concentrations. The highest population was found in P0 (0 g/L) with 1,42×1013 CFU/mL with growth decreasing as propineb concentration increased, reaching the lowest level of 3,22×10¹⁰ CFU/mL in P4 (2.00 g/L). In the degradation assay, Colletotrichum sp. colony diameter decreased from 3,38 cm in P0 (0 g/L) to 2,24 cm in P4 (2,00 g/L). Meanwhile, THR values increased consistently with fungicide concentration, rising from 3,17% in P0 (0 g/L) to 35,48% in P4 (2,00 g/L).
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Arunachalam, S. K., Reddy, N., Reddy, K., Devi, M., Kumar, A., Reddy, N., & Reddy, K. (2007). Evaluation of fungicidal resistance among Colletotrichum gloeosporioides isolates causing mango anthracnose in agri export zone of andhra pradesh, india. Plant Pathol. Bull., 16.
Awalliyah, A., Yulianty, Y., Salman, F., & Bambang, I. (2024). Pengaruh ekstrak etanol daun mahoni (Swietenia mahogani L.) sebagai fungisida nabati dalam mengendalikan Colletotrichum acutatum penyebab antraknosa pada cabai merah (Capsicum annuum L.). Jurnal Ilmiah Hijau Cendekia, 9(1), 8. https://doi.org/10.32503/hijau.v9i1.4127
Bose, S., Kumar, P. S., Vo, D. V. N., Rajamohan, N., & Saravanan, R. (2021). Microbial degradation of recalcitrant pesticides: a review. In Environmental Chemistry Letters (Vol. 19, Issue 4, pp. 3209–3228). Springer Science and Business Media Deutschland GmbH. https://doi.org/10.1007/s10311-021-01236-5
Elnahas, M. O., Hou, L., Wall, J. D., & Majumder, E. L. W. (2021). Bioremediation potential of Streptomyces sp. MOE6 for toxic metals and oil. Polysaccharides, 2(1), 47–68. https://doi.org/10.3390/polysaccharides2010004
Fitriana, I., Suryaminarsih, P., Mindari, W., & Wiyatiningsih, S. (2020). Studi pertumbuhan multiantagonis Trichoderma sp. dan Streptomyces sp. dalam suspensi akar, humat cair dan ekstrak kentang gula. Berkala Ilmiah Agroteknologi - Plumula, 7, 25–32. https://doi.org/10.33005/plumula.v7i1.19
Hajijah, H., Mariana, M., & Pramudi, M. I. (2022). Uji resistensi Colletotrichum sp. asal cabai hiyung terhadap fungisida berbahan aktif klorotalonil dan mankozeb. Jurnal Proteksi Tanaman Tropika, 5(2), 455–465. https://doi.org/10.20527/jptt.v5i2.1250
Hartati, S., Rahmah, S. A., & Nasahi, C. (2025). Inhibition of the in vitro growth of Colletotrichum sp. the cause of anthracnose on avocado fruit by yeast. CROPSAVER - Journal of Plant Protection, 8(1), 19–27. https://doi.org/10.24198/cropsaver.v8i1.63318
Indrawan, A. D., Suryaminarsih, P., & Mujoko, T. (2021, December 7). Prospect of Utilization of Microorganisms Streptomyces sp. and Trichoderma sp. in Supporting Sustainable Agriculture in the Age of Modern Agriculture. https://doi.org/10.11594/nstp.2021.1506
Laili, N. H., Abida, I. W., & Junaedi, A. S. (2022). Nilai total plate count (TPC) dan jumlah jenis bakteri air limbah cucian garam (bittern) dari tambak garam desa banyuajuh kecamatan kamal kabupaten bangkalan. Juvenil:Jurnal Ilmiah Kelautan Dan Perikanan, 3(1), 26–31. https://doi.org/10.21107/juvenil.v3i1.15075
Maeswari, D. N. D., Mujoko, T., Rahmadhini, N., & Raharjo, L. A. (2025). Uji antagonis Streptomyces spp. terhadap Sporisorium scitamineum penyebab luka api secara in vitro. Jurnal Agrotek Tropika, 13(1), 38. https://doi.org/10.23960/jat.v13i1.8532
Mahendra, M. I., Martosudiro, M., & Choliq, F. A. (2022). Eksplorasi jamur tanaj yang berpotensi sebagai bioremediator fungisida berbahan aktif propineb pada tanaman jeruk (Citrus reticulata L.). Jurnal Hama Dan Penyakit Tumbuhan, 10(4), 174–186. https://doi.org/10.21776/ub.jurnalhpt.2022.010.4.3
Noviana, D. P. D. (2018). Eksplorasi dan uji potensi khamir sebagai agens pendegradasi fungisida berbahan aktif metil tiofanat secara in vitro. Universitas Brawijaya.
Oktavia, N. D., Moelyaningrum, A. D., & Pujiati, R. S. (2015). Penggunaan pestisida dan kandungan residu pada tanah dan buah semangka (Citrullus vulgaris, Schard) (studi di kelompok tani subur jaya desa mojosari kecamatan puger kabupaten jember). Artikel Ilmiah Hasil Penelitian Mahasiswa.
Prajawahyudo, T., Asiaka, F. K. P., & Ludang, E. (2022). Peranan keamanan pestisida di bidang pertanian bagi petani dan lingkungan. J-SEA (Journal Socio Economics Agricultural), 17(1), 1.
Prakoso, N. B., Aprilina, V., & Qintharah, Y. N. (2023). Pemanfaatan bahan dapur sebagai penanggulangan hama untuk mengurangi ketergantungan terhadap pestisida kimia. Pena Dimas: Jurnal Pengabdian Masyarakat, 1(2), 51–58. https://doi.org/10.33474/p
Rahmansyah, M., Agustiyani, D., Julistiono, H., & Dewi, T. (2013). Growth and adaptation of four Streptomyces isolates in the media containing propxur. Journal of Agriculturen and Biological Science, 7(9), 773–781.
Sinambela, B. R. (2024). Dampak penggunaan pestisida dalam kegiatan pertanian terhadap lingkungan hidup dan kesehatan. AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 8(2), 178–187. https://doi.org/10.33096/agrotek.v8i2.625
Sulistinah, N., Antonius, S., & Rahmansyah, M. (2016). Pengaruh residu pestisida terhadap pola populasi bakteri dan fungi tanah di rumahkaca. Jurnal Teknologi Lingkungan, 12(1), 43. https://doi.org/10.29122/jtl.v12i1.1261
Suyanto, A., Oktarianti, S., Astar, I., & Tutik Purwani Irianti, A. (2022). Penggunaan Streptomyces ambofaciens sebagai bioaktivator dalam pembuatan pupuk organik cair dari limbah organik. Jurnal Teknotan, 16(1), 1. https://doi.org/10.24198/jt.vol16n1.1
Titalianingtyas, E., & Ratnasari, E. (2023). Aktivitas biofungisida ekstrak daun jarak pagar (Jatropha curcas L.) terhadap pertumbuhan Fusarium sp. secara in vitro. LenteraBio : Berkala Ilmiah Biologi, 12(2), 107–114. https://doi.org/10.26740/lenterabio.v12n2.p107-114
Utami, D. S., Priyani, N., & Munir, E. (2013). Isolasi dan uji potensi bakteri tanah pertanian berastagi sumatera utara dalam mendegradasi fungisida antracol berbahan aktif propineb. Saintia Biologi, 1(2), 8–14.
Wisnujatia, N. S., & Sangadji, S. S. (2021). Pengelolaan penggunaan pestisida dalam mendukung pembangunan berkelanjutan di Indonesia. SEPA: Jurnal Sosial Ekonomi Pertanian Dan Agribisnis, 18(1), 92. https://doi.org/10.20961/sepa.v18i1.47297
Yuka, R. A., Setyawan, A., & Supono, S. (2021). Identifikasi bakteri bioremediasi pendegradasi total amonia nitrogen (TAN). Jurnal Kelautan: Indonesian Journal of Marine Science and Technology, 14(1), 20–29. https://doi.org/10.21107/jk.v14i1.8499
DOI: https://doi.org/10.24198/cropsaver.v8i2.66871
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