KORELASI KADAR SERUM IFN-γ, EKSPRESI DAN FUNGSI RESEPTOR IFN-γ, DENGAN KEJADIAN TUBERKULOSIS PARU (The Correlation Of Ifn-γ Serum Level, Ifn-γ Receptor Expression And Function, With Pulmonary Tuberculosis)

Abstract

Abstrak

Untuk dapat bekerja dengan efektif menghadapi M. tuberculosis, sistim imunitas seluler memerlukan kadar IFN-γ dan reseptor IFN-γ di sel-sel mononuklear yang bekerja optimal. Tujuan penelitian ini adalah menganalisis korelasi antara kadar serum IFN-, ekspresi dan fungsi reseptor IFN-γ, dengan kejadian penyakit Tuberkulosis Paru. Observasi analitik dengan rancangan potong silang yang membandingkan kadar serum IFN-γ, fungsi dan ekspresi reseptor IFN-γ, pada pasien TB Paru dengan pasangannya yang sehat, serta menganalisis korelasi antara variabel-variabel tersebut dengan kejadian penyakit TB Paru. Penelitian dilakukan pada Juli 2009 sampai September 2010, di RS Immanuel Bandung dan laboratorium Stem Cell & Cancer Institute, Jakarta. Dibandingkan pasangan hidupnya, kadar serum IFN-γ pasien TB tidak berbeda bermakna, ekspresi reseptor IFN-γ pasien TB lebih tinggi (p=0,041), sedangkan fungsi reseptor IFN-γ pasien TB lebih rendah (p=0,011). Analisis korelasi mendapatkan satusatunya variabel yang mempunyai korelasi bermakna dengan kejadian tuberkulosis paru adalah fungsi reseptor IFN-γ yang rendah (p=0,026, OR 5,56). Pada pasien TB Paru ekspresi reseptor IFN-γ lebih tinggi, tetapi fungsi reseptor IFN-γ lebih rendah dari pasangan hidup sehat. Fungsi reseptor IFN-γ yang rendah ini mempunyai korelasi bermakna dengan kejadian tuberkulosis paru.

Kata kunci: tuberkulosis, kadar serum IFN-γ, ekspresi dan fungsi reseptor IFN-γ.

Abstract

In order to work effectively against M. tuberculosis, the cell mediated immune system needs serum level of IFN-γ and its receptors in the surface of mononuclear cells to function optimally. The objective of this study is to analyze the correlation of IFNγ serum level, expression and function of IFN-γ receptor, with pulmonary tuberculosis. Analytical descriptive method with cross sectional design that compared the serum level of IFN-γ, function and expression of IFN-γ receptor in pulmonary tuberculosis patients with their healthy spouses, and analyzing the correlation between these variables with pulmonary tuberculosis. Study was done from July 2009 until September 2010, in Immanuel hospital Bandung and Stem Cell & Cancer Institute Jakarta. Compared with their healthy spouses the IFN-γ serum level in TB patients was not different statistically, the IFN-γ receptor expression in TB patients was higher (p=0,041), however, the IFN-γ receptor function of TB patients was lower (p=0,011). Correlation analysis showed that the only variable had correlation significantly with pulmonary tuberculosis was low function of IFN-γ receptor (p=0,026,OR 5,56). The expression of IFN-γ receptor in pulmonary tuberculosis patients was higher, while the function of IFN-γ receptor was lower than their healthy spouses. The low function of IFN-γ receptor had significantly correlation with pulmonary tuberculosis.

Key Words: Tuberculosis, IFN-γ serum level, IFN-γ receptor expression and function.