Pengujian Ekstrak Etanol Daun Manggu Leuweung (Garcinia celebica L.) terhadap Proliferasi Lini Sel CCL-171

Putri Maharani, Elma N. M. R. Sahila, Raden Maya Febriyanti, Melisa Intan Barliana

Abstrak


Sistem imun merupakan suatu sistem kompleks yang bertindak sebagai pertahanan tubuh yang secara umum bekerja dengan mempertahankan homeostasis, mempersiapkan tubuh dalam melawan infeksi, dan penyembuhan. Namun, beberapa faktor seperti faktor endogen atau eksogen dapat mengubah efisiensi dan efektivitas dari sistem imun. Imunomodulator merupakan suatu senyawa yang dapat digunakan untuk membantu mempertahankan homeostasis dengan mengatur sistem sistem imun serta mengoptimalkannya. Pada penelitian terdahulu, telah ditunjukkan bahwa senyawa flavonoid yang terkandung dalam daun G. celebica L. dapat berpotensi sebagai agen anti inflamasi yang baik dengan mereduksi ekspresi sitokin proinflamasi. Selain itu, daun G. celebica L. juga mengandung senyawa terpenoid yang berperan sebagai antioksidan yang dapat menangkal radikal bebas. Pada penelitian ini dilakukan pengujian terhadap ekstrak etanol dan fraksi etil asetat daun manggu leuweung terhadap respon imun dengan menggunakan sel fibroblas paru normal CCL-171. Hasil penelitian menunjukkan bahwa ekstrak etanol mampu meningkatkan proliferasi dengan mempertahankan viabilitas sel yang ditunjukkan dengan % survival rate pada sel CCL-171 pada konsentrasi 7,8125; 15,625 dan 31,25 ppm secara berturut-turut adalah sebagai berikut 92,34 ± 5,77%; 90,20 ± 3,05%; 82,96 ± 4,58%


Kata Kunci


Manggu leuweung, Garcinia celebica L., Imunomodulator, Aktivitas proliferasi, WST-8

Teks Lengkap:

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Referensi


Soares MP, Teixeira L, Moita LF. Disease Tolerance and Immunity in Host Protection Against Infection. Nat Rev Immunol. 2017 Feb 3;17(2):83–96.

Chauhan S, Jena KK, Mehto S, Chauhan NR, Sahu R, Dhar K, et al. Innate Immunity and Inflammophagy: Balancing the Defence and Immune Homeostasis. FEBS J. 2022 Jul 14;289(14):4112–31.

Chowdhury MA, Hossain N, Kashem MA, Shahid MdA, Alam A. Immune Response in COVID-19: A Review. J Infect Public Health. 2020 Nov;13(11):1619–29.

Hirayama D, Iida T, Nakase H. The Phagocytic Function of Macrophage-Enforcing Innate Immunity and Tissue Homeostasis. Int J Mol Sci. 2017 Dec 29;19(1):92.

Peter J. Delves, Seamus J. Martin, Dennis R. Burton, Ivan M. Roitt. Roitt’s Essential Immunology. 13th ed. Oxford: John Wiley and Sons; 2017.

Brunton LL, Lazo JS, Parker KL, editors. Goodman & Gilman’s The Pharmacological Basis of Therapeutics. 11th ed. New York: McGraw-Hill; 2006.

Catanzaro M, Corsini E, Rosini M, Racchi M, Lanni C. Immunomodulators Inspired by Nature: A Review on Curcumin and Echinacea. Molecules. 2018 Oct 26;23(11):2778.

Shukla S, Bajpai VK, Kim M. Plants as potential sources of natural immunomodulators. Vol. 13, Reviews in Environmental Science and Biotechnology. 2014. p. 17–33.

Jantan I, Ahmad W, Bukhari SNA. Plant-derived immunomodulators: An insight on their preclinical evaluation and clinical trials. Vol. 6, Frontiers in Plant Science. Frontiers Research Foundation; 2015.

Pasaribu YP, Fadlan A, Fatmawati S, Ersam T. Biological activity evaluation and in silico studies of polyprenylated benzophenones from garcinia celebica. Biomedicines. 2021 Nov 1;9(11).

Latief M. KARAKTERISASI SENYAWA DARI FRAKSI AKTIF ANTIBAKTERI DAUN Garcinia celebica linn (Kandis). 2015;1(1):1–6.

Subarnas A, Diantini A, Abdulah R, Zuhrotun A, Nugraha PA, Hadisaputri YE, et al. Apoptosis-mediated antiproliferative activity of friedolanostane triterpenoid isolated from the leaves of Garcinia celebica against MCF-7 human breast cancer cell lines. Biomed Rep. 2016 Jan 1;4(1):79–82.

Ansori A, Fadholly A, Hayaza S, Susilo R, Inayatilllah B, Winarni D, et al. A Review on Medicinal Properties of Mangosteen (Garcinia mangostana L.). Research Journal of Pharmacy and Technology . 2020;13:974–82.

Purbowati R, Ersam T. Exploration of Phenolic Compound from The Stem Bark of Garcinia latissima Miq. Jurnal Sains dan Seni ITS. 2019;8(2):12–4.

Martel J, Ojcius DM, Chang CJ, Lin CS, Lu CC, Ko YF, et al. Anti-obesogenic and antidiabetic effects of plants and mushrooms. Vol. 13, Nature Reviews Endocrinology. Nature Publishing Group; 2017. p. 149–60.

Kurniawan A, Parikesit. Tree species distribution along the environmental gradients in Pananjung Pangandaran Nature Reserve, West Java. Biodiversitas [Internet]. 2008 Sep 16;9(4). Available from: https://smujo.id/biodiv/article/view/357

Tan WN, Tan ZH, Zulkifli NI, Nik Mohamed Kamal NNS, Rozman NAS, Tong WY, et al. Sesquiterpenes rich essential oil from Garcinia celebica L. and its cytotoxic and antimicrobial activities. Nat Prod Res. 2020;34(23):3404–8.

Widyowati R, Rahman A. Kandungan Kimia dan Aktivitas Antimikroba Ekstrak Garcinia Celebica l. terhadap Staphylococcus Aureus, Shigella Dysenteriae dan Candida Albicans. Majalah Farmasi Airlangga. 2010;8(2):23–7.

Guiotto M, Raffoul W, Hart AM, Riehle MO, Di Summa PG. Human platelet lysate to substitute fetal bovine serum in hMSC expansion for translational applications: A systematic review. Vol. 18, Journal of Translational Medicine. BioMed Central Ltd; 2020.

ECACC. CO2 concentration and pH control in the cell culture laboratory [Internet]. 2019. Available from: https://www.culturecollections.org.uk/news/ecacc-news/co2-concentration-and-ph-control-in-the-cell-culture-laboratory.aspx#:~:text=CO2%20is%20not%20a,stable%20physiological%20pH%20through%20the

ECACC. General Cell Collection Detail ECACC General Cell Collection: MRC-5 pd13 European Collection of Authenticated Cell Cultures (ECACC) ECACC General Collection MRC-5 pd13 [Internet]. 2022. Available from: www.nibsc.org

Merck. Cell Viability and Proliferation Assays [Internet]. Merck. 2022 [cited 2022 Sep 2]. Available from: https://www.sigmaaldrich.com/ID/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/imaging-analysis-and-live-cell-imaging/cell-viability-and-proliferation

Dojindo. Cell Counting Kit-8 Technical Manual Cell Proliferation Assay and Cytotoxicity Assay [Internet]. 2016. Available from: http://www.dojindo.eu.com/

Karakaya G, Ercan A, Öncül S, Aytemir MD. Kojic acid derivatives as potential anticancer agents: Synthesis and cytotoxic evaluation on A375 human malignant melanoma cells. Journal of Research in Pharmacy. 2019;23(4):596–607.

Da Costa JP, Rodrigues APD, Farias LHS, Frade PCR, Da Silva BJM, Do Nascimento JLM, et al. Biological effects of kojic acid on human monocytes in vitro. Biomedicine and Pharmacotherapy. 2018 May 1;101:100–6.

Nguyen NH, Hoai Ta QT, Pham QT, Han Luong TN, Phung VT, Duong TH, et al. Anticancer activity of novel plant extracts and compounds from Adenosma bracteosum (Bonati) in human lung and liver cancer cells. Molecules. 2020 Jun 1;25(12).

Haryoto, Muhtadi, Indrayudha P, Azizah T, Suhendi A, Pos T. Aktivitas Sitotoksik Ekstrak Etanol (Haryoto dkk) AKTIVITAS SITOTOKSIK EKSTRAK ETANOL TUMBUHAN SALA (Cynometra ramiflora Linn) TERHADAP SEL HeLa, T47D dan WiDR. Jurnal Penelitian Saintek. 2013;18(2):21–8.

Serafini M, Peluso I, Raguzzini A. Flavonoids as anti-inflammatory agents. In: Proceedings of the Nutrition Society. 2010. p. 273–8.

Singh N, Tailang M, Mehta SC. A REVIEW ON HERBAL PLANTS AS IMMUNOMODULATORS. Int J Pharm Sci Res [Internet]. 2016;7(9):3602. Available from: http://dx.doi.org/10.13040/IJPSR.0975-8232.7




DOI: https://doi.org/10.24198/ijbp.v3i2.47089

DOI (PDF): https://doi.org/10.24198/ijbp.v3i2.47089.g21239

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