POTENSI LIMBAH KULIT JERUK NIPIS (Citrus auronfolia) SEBAGAI INHIBITOR TIROSINASE

Siti Hindun, Taofik Rusdiana, Marline Abdasah, Reti Hindritiani

Abstrak


Hiperpigmentasi merupakan kelainan kulit wajah yang umum terjadi, terutama karena adanya peningkatan melanogenesis, dengan gambaran berupa warna kulit menjadi hitam atau coklat kehitaman. Kelainan ini terdapat pada beberapa macam penyakit kulit diantaranya melasma, melanoderma paska inflamasi, lentigo solaris dan freckles. Salah satu prinsip penanganan hiperpigmentasi yaitu menghambat sintesis melanin yang dapat dilakukan dengan menggunakan agen depigmentasi yang mekanisme kerjanya menghambat aktivitas enzim tirosinase. Penelitian ini bertujuan untuk menguji potensi kulit jeruk nipis sebagai inhibitor tirosinase. Kulit jeruk nipis diektraksi dengan etanol 96 %, identifikasi flavonoid, menghitung flavonoid total, kemudian diuji inhibisi tirosinase menggunakan instrumen microplate reader (ELISA). Hasil penelitian ekstrak kulit jeruk mengandung flavonid, dengan total flavonid totalnya 0,667 % b/b dan inhibition concentration (IC) 50 42,11 mg/mL, Kulit jeruk berpotensi sebagai inhibisi tirosinase.

Kata kunci:    Citrus auronfolia, Hiperpigmentasi, IC50, Tirosinase


Teks Lengkap:

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Referensi


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DOI: https://doi.org/10.15416/ijpst.v4i2.12642

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