Crystallization and Biological Studies of Nypa fruticans Wurmb Sap

Sukairi A. Hafizi, Asaruddin M. Razip, Wan S. W. M. Aliana, Wan Y. S. A. Tarmizi

Abstrak


Nypa fruticans is a standout amongst the most broadly appropriated and helpful palm in the mangrove backwoods in the Southeast Asia. The sap of this plant is produced at the fruit stalks or called as ower stalks or in orescence. Nipa sap contains a lot of chemical constituents that can be used other alter- native to produce sugar that give a lot bene ts. The crystallization process of this sap was conducted in controlled temperature, pressure and humidity. As the crystal formed, remaining liquid was tested for biological studies which are antidiabetic, antioxidant and toxicity test. The antidiabetic properties were determined by inhibition of α-amylase and the result was compared to the synthetic drug which is acarbose. The sample showed that a high property of antidiabetic (50 mg/mL= 78.50% inhibition) compared to the acarbose (50 mg/mL= 80.90% inhibition). Meanwhile, the antioxidant properties of the sample were determined by2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. The sample also showed a positive result which is EC50 112.90 mg/mL. Lastly, the toxicity test was tested to brine shrimp (Artemia salina) to get the LC50. The result (LC50=271.7 mg/mL) showed low toxic- ity at different concentration of the sample. From this study, the sample had potential antioxidant and antidiabetic properties, with low toxicity.

Key words: α-amylase, antioxidant, Artemia salina, Nypa fruticans, toxicity test


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Referensi


Tamunaidu P, Saka S. Chemical charac- terization of various parts of nipa palm (Nypa fruticans). Industrial Crops and Products. 2011 Nov 30;34(3):1423-8.

Bandaranayake WM. Traditional and me- dicinal uses of mangroves. Mangroves and salt marshes. 1998 Sep 1;2(3):133- 48.

Yusoff NA, Yam MF, Beh HK, Razak KN, Widyawati T, Mahmud R, Ahmad M, As- mawi MZ. Antidiabetic and antioxidant activities of Nypa fruticans Wurmb. vin- egar sample from Malaysia. Asian Paci c journal of tropical medicine. 2015 Aug 31;8(8):595-605.

Triastuti A, Park HJ, Choi JW. Phaleria- macrocarpa suppress nephropathy by in- creasing renal antioxidant enzyme activity in alloxan-induced diabetic rats. Natural Products Sciences. 2009;15(3):167-72.

Tamil IG, Dineshkumar B, Nandhakumar M, Senthilkumar M, Mitra A. In vitro study on α-amylase inhibitory activity of an Indian medicinal plant, Phyllanthus

7

amarus. Indian journal of pharmacology.

Oct;42(5):280.

Lugemwa FN, Snyder AL, Shaikh K. De-

termination of radical scavenging activity and total phenols of wine and spices: A randomized study. Antioxidants. 2013 Jul 25;2(3):110-21.

Moshi MJ, Innocent E, Magadula JJ, Otieno DF, Weisheit A, Mbabazi PK, Nondo RS. Brine shrimp toxicity of some plants used as traditional medicines in Kagera region, north western Tanzania. Tanzania Journal of Health Research. 2010;12(1):63-7.

Sim L, Jayakanthan K, Mohan S, Nasi R, Johnston BD, Pinto BM, Rose DR. New glucosidase inhibitors from an ayurvedic herbal treatment for type 2 diabetes: struc- tures and inhibition of human intestinal maltase-glucoamylase with compounds from Salacia reticulata. Biochemistry. 2009 Dec 29;49(3):443-51.

Oyedemi S, Koekemoer T, Bradley G, van de Venter M, Afolayan A. In vitro an- ti-hyperglycemia properties of the aque- ous stem bark extract from Strychnoshen- ningsii (Gilg). International Journal of Diabetes in Developing Countries. 2013 Jun 1;33(2):120-7.

Kim JS, Kwon CS, Son KH. Inhibition of alpha-glucosidase and amylase by luteo- lin, a avonoid. Bioscience, biotechnolo- gy, and biochemistry. 2000;64(11):2458- 61.

Lugemwa FN, Snyder AL, Shaikh K. De- termination of radical scavenging activity and total phenols of wine and spices: A randomized study. Antioxidants. 2013 Jul 25;2(3):110-21.

Al Kazaz M, Desseaux V, Marchis- Mouren G, Prodanov E, Santimone M. The mechanism of porcine pancreatic al- pha-amylase. Inhibition of maltopentaose hydrolysis by acarbose, maltose and mal- totriose. European journal of biochemis- try. 1998 Feb;252(1):100-7.

Sowndhararajan K, Kang SC. Free radi- cal scavenging activity from different ex- tracts of leaves of Bauhinia vahlii Wight &Arn. Saudi journal of biological scienc- es. 2013 Oct 31;20(4):319-25.

Wu C. An important player in brine shrimp lethality bioassay: The solvent. Journal of advanced pharmaceutical tech- nology & research. 2014 Jan;5(1):57.

Mbwambo ZH, Moshi MJ, Masimba PJ, Kapingu MC, Nondo RS. Antimicrobial activity and brine shrimp toxicity of ex- tracts of Terminaliabrownii roots and stem. BMC complementary and Alterna- tive Medicine. 2007 Mar 30;7(1):9.

randomized study. Antioxidants. 2013 Jul 25;2(3):110-21.

Al Kazaz M, Desseaux V, Marchis- Mouren G, Prodanov E, Santimone M. The mechanism of porcine pancreatic al- pha-amylase. Inhibition of maltopentaose hydrolysis by acarbose, maltose and mal- totriose. European journal of biochemis- try. 1998 Feb;252(1):100-7.

Sowndhararajan K, Kang SC. Free radi- cal scavenging activity from different ex- tracts of leaves of Bauhinia vahlii Wight &Arn. Saudi journal of biological scienc- es. 2013 Oct 31;20(4):319-25.

Wu C. An important player in brine shrimp lethality bioassay: The solvent. Journal of advanced pharmaceutical tech- nology & research. 2014 Jan;5(1):57.

Mbwambo ZH, Moshi MJ, Masimba PJ, Kapingu MC, Nondo RS. Antimicrobial activity and brine shrimp toxicity of ex- tracts of Terminaliabrownii roots and stem. BMC complementary and Alterna- tive Medicine. 2007 Mar 30;7(1):9.




DOI: https://doi.org/10.24198/ijpst.v1i1.16122

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