Indonesian Journal of Pharmaceutical Science and Technology
Antioxidant Activity, Sun Protection Factor and Phytochemical Properties of Different Parts Sawo Walanda (Pouteria Campechiana (Kunth.) B.) Extract
Abstrak
Abstract
UV radiation can lead production of Reactive Oxygen Species (ROS) on the skin and can cause negative effects1. Sun protector and antioxidant ingredients are needed to protect the skin2. Phenolic compounds and flavonoids have been used as sun protectors and as antioxidants3. Pouteria campechiana is known for its high abundance of phenolic compounds4. This study reported the phytochemical group, total phenol and total flavonoid content, antioxidant activity, and sun protector factor of ethanol extracts of pulp, seed, leaves, and twigs of Pouteria campechiana. Determination of antioxidant activity, sun protection factor, and total phenol and total flavonoid content were carried out using UV-visible spectrophotometry. Crude drugs and extract of pulp, seed, twig, and leaves P.campechiana containing phenolic group, tannin, and flavonoid. The leaf extract had the highest total phenolic content, while the highest total flavonoid content was in the seed extract. All of the extracts tested had very strong antioxidant activity indicated by the Antioxidant Activity Index (AAI) to DPPH value, >2. At a concentration of 1000 µg/mL, the leaf extract showed the highest SPF value, 16.01 ± 0.38.The conclusion, the leaf extract had the potential to further as a natural antioxidant, and sun protector.
Abstrak
Paparan radiasi UV dapat meningkatkan produksi Reactive Oxygen Species (ROS) sebagai radikal bebas dan dapat menimbulkan efek negatif pada kulit. Dibutuhkan senayawa aktif sebagai fotoprotektor dan senyawa penangkal radikal bebas yang disebut antioksidan untuk melindungi kulit. Golongan senyawa fenol dan flavonoid dapat menyerap radiasi UV dan menangkal radikal bebas. Pouteria campechiana dilaporkan mengandung senyawa fenol. Penelitian ini melaporkan golongan senyawa kimia, kadar fenol dan flavonoid total, aktivitas antioksidan terhadap DPPH dan aktivitas fotoprotektor yang menggunakan parameter nilai Sun Protector Factor (SPF) dari ekstrak etanol daging buah, biji, ranting dan daun Poteria campechiana. Ativitas antioksidan, nilai SPF, kadar fenol total, dan flavonoid total diukur secara spektrofotometri UV-Sinar tampak. Hasil penelitin menunjukkan simplisia dan ekstrak etanol daging buah, biji, ranting dan daun P.campechiana mengandung golongan senyawa fenoli, tanin, dan flavonoid. Ekstrak etanol daun memiliki kadar fenol total tertinggi, sedangkan kadar flavonoid total tertinggi ada pada ekstrak etanol biji. Keempat ekstrak tergolong pada antioksidan sangat kuat, dengan parameter nilai AAI >2. Pada konsentrasi 1000 µg/mL, ekstrak daun menunjukkan nilai SPF tertinggi yaitu sebesar 16,01 ± 0,38. Kesimpulan, ekstrak etanol daun P.campechiana lebih berpotensi untuk dikembangkan sebagai bahan baku aktif antioksidan alami dan fotoprotektor.
Kata Kunci
Teks Lengkap:
PDFReferensi
Poudel B, Gurung A, Subedi HP, Babu S, and Prajuli ATK (2022) In vitro sun protection factor determination of selected medicinal plants and formulation of sunscreen cream. Systematic Review Pharmacy 13(7): 755-762.
Petruk G, Giudice RD, Rigano MM, Monti DM (2018) Antioxidants from plants protect against skin photoaging. Oxidative Medicine and Cellular Longevity. 18: 1-11. http://doi:10.1155/2018/1454936
Yang Y and Li S. Dandelion extracts protect human skin fibroblasts from UVB damage and cellular senescence. Oxidative Medicine and Cellular Longevity. 2015; 15: 12-22. http://doi:10.1155/2015/619560
Hawryluk EB, Oztan A, and Fisher DE. Effects of ultraviolet exposure behaviors on skin pigmentation and melanoma. Journal of Pigmentary Disorder. 2014; 1(2): 2-4. http://doi: 10.4172/jpd.1000113
Lohani A, Miasra AK, Verma A. Cosmeceutical potential of geranium and calendula essential oil: Determination of antioxidant ac-tivity and in vitro sun protection factor. Journal of Cosmetic Dermatology. 2018; 18: 163-172.
Elsayed A, El-tanbouly N, Moustafa S, Abdou R, Sally A, and Awdan W. Chemical composition and biological activities of Pouteria campechiana (Kunth.) Baehni. Academic Journal. 2016; 10(16): 209–215. http/:doi: 10.5897/JMPR2015.6031
Aseervatham G, Manthra V, Ireen C, Thilagameena S, Akshaya S, Clara MA, Giriprashanthini S, and Sivasudha T. Free radical scavenging potential and antihaemolytic activity of methanolic extract of Pouteria campechiana (Kunth) Baehni. and Tricosanthes tricuspidate. Biocatalysis and Agricultural Biotechnology. 2005; 18: 101031. http/:doi: 10.1016/j.bcab.2019.101031.
Fitriansyah SN, Fidrianny I, and Hartati R. Pharmacological activities and phytochemical compounds: Overview of Pouteria genus. Pharmacognosy Journal. 2021; 3(2): 577–584. http//doi: 10.5530/PJ.2021.13.72
Farnsworth NR. Biological and phytochemical screening of plants. Journal of Pharmaceutical Sciences. 1966; 55: 874–875. https://doi. org/10.1002/jps.2600550302
Sarker SD, Nahar L. Methods in Molecular BiologyTM Series Editor. Humana press, US, 350–358 pp. 2013
Pourmorad F, Hosseinimehr SJ and Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. African Journal of Biotechnology. 2006; 5(11): 1142-1145. http://www.academicjournals.org/AJB
Chang CC, Yang MH, Wen HM and Chem JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 2002; 10(3): 178-182. http://doi:0.38212/2224-6614.2748
Fidrianny I, Sari E, and Ruslan K. Phytochemical content and antioxidant activities in different organs of Pamelo (Citrus maxima (Burm.) Merr.) using 2,2-diphenyl-1-picrylhydrazyl and phosphomolydbenum assay. Asian Journal of Pharmaceutical and Clinical Research. 2016; 9(2): 185-190. http//doi: 10.22159/AJPCR.2016.V9S2.13526
Fitriansyah NF, Hartati R, Fidriannya I. Effect of different solvent on phytochemical content, tyrosinase inhibition and antioxidant activities of campolay (Pouteria campechiana Kunth. Baehni). Open Access Macedonian Journal of Medical Science. 2022; 10(A): 158-163.
Scherer R and Godoy HT. Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method. Food Chemistry. 2009; 12: 654-658. http://doi:10.1016/j.foodchem.2008.06.026
More BH, Sakharwade SN, Tembhurne SV, Sakarkar DM. Evaluation of sunscreen activity of cream containing leaves extract of Butea monosperma for topical application. International Jurnal Cosmetic Science. 2013; 3:1-6
Permana, A.D.; Utami, R.N.; Courtenay, A.J.; Manggau, M.A.; Donnelly, R.F.; Rahman, L. Phytosomal Nanocarriers as Platforms for Improved Delivery of Natural Antioxidant and Photoprotective Compounds in Propolis: An Approach for Enhanced Both Dissolution Behaviour in Biorelevant Media and Skin Retention Profiles. Journal of Phytochemistry and Photobiology B: Biology. 2020; 205: 111846
Ebrahimzadeh MA, Enayatifard R, Khalili M, Ghaffarloo M, Saeedi M, and Charati JY. Correlation between sun protection factor and antioxidant activity, phenol and flavonoid contents of some medicinal plants. Iranian Journal of Pharmaceutical Research. 2014; 13(3): 1041-1047, http:// ijpr.sbmu.ac.ir
Costa SC, Detoni CB, Branco CR, Botura MB, Branco A. In vitro photoprotective effects of Marcetia taxifolia ethanolic extract and its potential for sunscreen formulations. Revista Brasileira de Farmacognosia. 2015; 25: 413-418. https://doi.org/10.1016/j.bjp.2015.07.013
Ikram EHK, Eng KHK, Jalil AMM, Ismail A, Idris S, Azlan A, et al. Antioxidant capacity and total phenolic content of Malaysian underutilized fruits. Journal of Food Composition and Analysis. 2009; 22(5): 388–393.https://doi.org/10.1016/j.jfca.2009.04.001
Kubola J, Siriamornpun S, Meeso N. Phytochemicals, vitamin C and sugar content of Thai wild fruits. Food Chemistry. 2011; 126(3): 972-981.https://doi.org/10.1016/j.foodchem.2010.11.104
Adiyaman P, Kanchana S, Usharani T, Ilaiyaraja N, Kalaiselvan A, Kumar KRA. Identification and quantification of polyphenolic compounds in underutilized fruits (star fruit and egg fruit) using HPLC. Indian Journal of Traditional Knowledge. 2016; 5(03): 487–493.
Fernandez-Panchon MS, Villano D, Troncoso AM, and Garcia-Parrilla MC. Antioxidant activity of phenolic compounds: From in vitro results to in vivo evidence. Critical Review in Food Science and Nutrition. 2008; 48(7): 649–671. http//doi: 10.1080/10408390701761845.
Treml J and Smejkal K. Flavonoids as potent scavengers of hydroxyl radicals. Comprehensive Reviews in Food Science and Food Safety. 2016; 15(1): 720-738. http://doi:10.1111/1541-4337.12204
Baky MH, Kamal AK, Elgindi MR and Haggag EG. A review on phenolic compounds from family sapotaceae. Journal of Pharmacognosy and Phytochemistry. 2016; 5(2): 280-287
Ma J, Yang H, Basile MJ and Kennelly EJ. Analysis of polyphenolic antioxidants from the fruit of three Pouteria species by selected ion monitoring liquid chromatography-mass spectrometry. Journal of Agricultural and Food Chemistry. 2004; 52(19): 5873-5878. http://doi: 10.1021/jf049950k
Schalka S, Manoel V, and dos Reis S. Sun protection factor: meaning and controversies. Anais Brasileiros de Dermatologia. 2011; 86(3): 507-15
DOI: https://doi.org/10.24198/ijpst.v0i0.50584
Refbacks
- Saat ini tidak ada refbacks.
| Switch to English Back to Top |
| View My Stats Penerbit Universitas Padjadjaran
Jurnal ini terindeks di : Creative Commons Attribution :
Based on a work at http://jurnal.unpad.ac.id/ijpst/ |
