Senyawa Non-aromatik Herba Centella asiatica menggunakan Metode Baru melalui Karbon Aktif, dan Analisis Farmakologi Jejaring pada Luka Bakar

Rizki Awaluddin; Rafli Mulkan Mustaqim; Dayar Arbain; Farida Hayati; Siti Khaerunnisa

doi:10.24198/ijpst.v11i2.50623

Senyawa Non-aromatik Herba Centella asiatica menggunakan Metode Baru melalui Karbon Aktif, dan Analisis Farmakologi Jejaring pada Luka Bakar

Rizki Awaluddin, Rafli Mulkan Mustaqim, Dayar Arbain, Farida Hayati, Siti Khaerunnisa

Abstrak

Prevalensi luka bakar masih menjadi perhatian kaitannya dengan perubahan fisik pasca luka bakar yakni contractures, hypertrophic scar dan keloid yang menurunkan fungsi, kualitas hidup dan estetika kulit. Herba pegagan (Centella asiatica) melalui senyawa triterpen telah dikenal untuk mempercepat luka bakar. Namun, sifat senyawa triterpen yang polar identik dengan keberadaan klorofil dan senyawa lainnya yang sulit dipisahkan. Masih adanya kontroversi terkait protein yang berperan dalam kesembuhan luka bakar membutuhkan kajian untuk menentukan protein potensial. Penelitian ini bertujuan untuk mengeksplorasi metode baru pada ekstraksi herba pegagan menggunakan karbon aktif serja kajian farmakologi jejaring untuk menentukan protein potensial. Herba pegagan diekstraksi menggunakan metode maserasi pada pelarut metanol dan dilanjutkan dengan penambahan karbon aktif. Hasil purifikasi diidentifikasi menggunakan KLT dengan fase gerak kloroform: asam asetat glasial: metanol: akuades (60:32:12:8 v/v) dan pereaksi semprot anisaldehid-asam sulfat. Kajian farmakologi jejaring dilakukan secara in silico untuk mengkaji interaksi protein-protein dan senyawa-protein berkaitan dengan luka bakar. Hasil skrining fitokimia menunjukkan keberadaan asaticoside pada ekstrak terpurifikasi dan maserat dengan nilai Rf yang relatif identik. Senyawa non aromatik berperan dalam mempercepat kesembuhan luka bakar dengan mempengaruhi protein IL1β, IL6, MAPK8, PTGS2, NOX4, IFN-γ, NFKB1, TGFβ1, MMP13, dan TNF.

Kata Kunci

luka bakar, ekstrak terpurifikasi, karbon aktif, triterpen, Centella asiatica

Teks Lengkap:

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Referensi

Wardhana A, Basuki A, Prameswara ADH, Rizkita DN, Andarie AA, Canintika AF. The epidemiology of burns in Indonesia’s national referral burn center from 2013 to 2015. Burn Open [Internet]. 2017;1(2):67–73. Available from: https://doi.org/10.1016/j.burnso.2017.08.002

Utami AM, Elfiah U, Wisudanti DD. Assessments of quality of life in burn patients after hospital treatment in Jember. J Kedokt dan Kesehat Indones. 2020;11(2):157–64.

Spronk I, Legemate CM, Dokter J, van Loey NEE, van Baar ME, Polinder S. Predictors of health-related quality of life after burn injuries: A systematic review. Crit Care. 2018;22(1):1–13.

Spronk I, Legemate C, Oen I, van Loey N, Polinder S, van Baar M. Health related quality of life in adults after burn injuries: A systematic review. PLoS One. 2018;13(5):1–21.

Somboonwong J, Kankaisre M, Tantisira B, Tantisira MH. Wound healing activities of different extracts of Centella asiatica in incision and burn wound models : an experimental animal study. BMC Complement Altern Med [Internet]. 2012;12(1):1. Available from: BMC Complementary and Alternative Medicine

Awaluddin R, Nugrahaningsih DAA, Solikhah EN. The concentration-dependent pro-fibrotic effect of metformin on LPS and high glucose induced fibroblast NIH 3T3 and macrophage raw 264.7 cell co-culture. Med J Malaysia. 2020;75(May):10–3.

Awaluddin R, Nugrahaningsih DAA, Solikhah EN, Chabib L. The Effect of Asiatic Acid and Metformin on the Viability Percentage of Mouse Macrophage Cell Lines RAW264.7 and Mouse Fibroblast Cell Lines NIH3T3. IOP Conf Ser Earth Environ Sci. 2020;448(1).

Wu F, Bian D, Xia Y, Gong Z, Tan Q, Chen J, et al. Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs. 2012;2012.

Abbas manal mohammad, Ar-rawi N, Abbas M ahmad, Al-khateeb I. Naringenin potentiated β-sitosterol healing effect on stracth wound assay. Res Pharm Sci. 2019;14(6):566–73.

Jewo PI, Fadeyibi IO, Babalola OS, Saalu LC, Benebo AS, Izegbu MC, et al. A Comparative Study of the Wound Healing Properties of Moist Exposed Burn Ointment (MEBO) and Silver Sulphadiazine. Ann Burns Fire Disasters [Internet]. 2009;22(2):79–82. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21991159%0Ahttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC3188221

Küpeli Akkol E, Renda G, İlhan M, Bektaş NY. Wound healing acceleration and anti-inflammatory potential of Prunella vulgaris L.: From conventional use to preclinical scientific verification. J Ethnopharmacol. 2022 Sep 15;295:115411.

Seong E, Heo H, Sang Jeong H, Lee H, Lee J. Enhancement of bioactive compounds and biological activities of Centella asiatica through ultrasound treatment. Ultrason Sonochem [Internet]. 2023;94(March):106353. Available from: https://doi.org/10.1016/j.ultsonch.2023.106353

Oesterle P, Lindberg RH, Fick J, Jansson S. Extraction of active pharmaceutical ingredients from simulated spent activated carbonaceous adsorbents. Environ Sci Pollut Res. 2020;27(20):25572–81.

Chong NJ, Aziz Z. A systematic review on the chemical constituents of Centella asiatica. Res J Pharm Biol Chem Sci. 2011;2(3):445–59.

James J, Dubery I. Identification and quantification of triterpenoid centelloids in Centella asiatica (L.) Urban by densitometric TLC. J Planar Chromatogr - Mod TLC. 2011;24(1):82–7.

Nursamsiar N, M. Mangande M, Awaluddin A, Nur S, Asnawi A. In Silico Study of Aglycon Curculigoside A and Its Derivatives as α-Amilase Inhibitors. Indones J Pharm Sci Technol. 2020;7(1):29.

Rubianto Tjandrawinata R, Winda Amalia A, Tuna H, Novalinda Saidi V, Tan S, Tradisional Tiongkok P, et al. Mekanisme Molekuler Imunomodulasi oleh Huangqi (Astragali Radix) Menggunakan Sistem Farmakologi Jejaring (Molecular Mechanisms of Network Pharmacology-Based Immunomodulation of Huangqi (Astragali Radix)). J Ilmu Kefarmasian Indones [Internet]. 2022;20(2):184–95. Available from: https://www.ncbi.nlm.nih.gov/gene/

Fan J, Zhou J, Qu Z, Peng H, Meng S, Peng Y, et al. Network Pharmacology and Molecular Docking Elucidate the Pharmacological Mechanism of the OSTEOWONDER Capsule for Treating Osteoporosis. Front Genet. 2022;13(February):1–13.

Gaydukova AM, Nenasheva AS, Kolesnikov VA, Vetlugin NA. The Extraction of Activated Carbon from Aqueous Solutions in the Presence of Organic and Inorganic Compounds by the Electroflotation Method. J Water Chem Technol 2021 432 [Internet]. 2021 Jun 8 [cited 2023 Oct 19];43(2):116–22. Available from: https://link.springer.com/article/10.3103/S1063455X21020077

Sun B, Wu L, Wu Y, Zhang C, Qin L, Hayashi M, et al. Therapeutic Potential of Centella asiatica and Its Triterpenes: A Review. Front Pharmacol. 2020;11(September):1–24.

Sujiono EH, Zabrian D, Zurnansyah, Mulyati, Zharvan V, Samnur, et al. Fabrication and characterization of coconut shell activated carbon using variation chemical activation for wastewater treatment application. Results Chem [Internet]. 2022;4(November 2021):100291. Available from: https://doi.org/10.1016/j.rechem.2022.100291

Jawad AH, Abdulhameed AS, Mastuli MS. Mesoporous Crosslinked Chitosan-Activated Charcoal Composite for the Removal of Thionine Cationic Dye: Comprehensive Adsorption and Mechanism Study. J Polym Environ [Internet]. 2020 Mar 1 [cited 2023 Oct 20];28(3):1095–105. Available from: https://link.springer.com/article/10.1007/s10924-020-01671-5

Agatonovic-Kustrin S, Kustrin E, Gegechkori V, Morton DW. High-performance thin-layer chromatography hyphenated with microchemical and biochemical derivatizations in bioactivity profiling of marine species. Mar Drugs. 2019;17(3):1–14.

Penatzer JA, Srinivas S, Thakkar RK. The role of macrophages in thermal injury. Int J Burns Trauma [Internet]. 2022 [cited 2023 Oct 20];12(1):1. Available from: /pmc/articles/PMC8918762/

Lateef Z, Stuart G, Jones N, Mercer A, Fleming S, Wise L. The cutaneous inflammatory response to thermal burn injury in a Murine model. Int J Mol Sci. 2019;20(3).

Diniz LRL, Calado LL, Duarte ABS, de Sousa DP. Centella asiatica and Its Metabolite Asiatic Acid: Wound Healing Effects and Therapeutic Potential. Metabolites. 2023;13(2).

Xu X, Si L, Xu J, Yi C, Wang F, Gu W, et al. Asiatic acid inhibits cardiac hypertrophy by blocking interleukin-1β-activated nuclear factor-κB signaling in vitro and in vivo. J Thorac Dis. 2015;7(10):1787–97.

Lee JH, Kim HL, Lee MH, You KE, Kwon BJ, Seo HJ, et al. Asiaticoside enhances normal human skin cell migration, attachment and growth in vitro wound healing model. Phytomedicine [Internet]. 2012 Oct 15 [cited 2023 Oct 20];19(13):1223–7. Available from: https://pubmed.ncbi.nlm.nih.gov/22939261/

Singkhorn S, Tantisira MH, Tanasawet S, Hutamekalin P, Wongtawatchai T, Sukketsiri W. Induction of keratinocyte migration by ECa 233 is mediated through FAK/Akt, ERK, and p38 MAPK signaling. Phyther Res [Internet]. 2018 Jul 1 [cited 2023 Oct 20];32(7):1397–403. Available from: https://onlinelibrary.wiley.com/doi/full/10.1002/ptr.6075

Korkmaz HI, Flokstra G, Waasdorp M, Pijpe A, Papendorp SG, de Jong E, et al. The Complexity of the Post-Burn Immune Response: An Overview of the Associated Local and Systemic Complications. Cells. 2023;12(3).

Bian D, Zhang J, Wu X, Dou Y, Yang Y, Tan Q, et al. Asiatic Acid Isolated From Centella Asiatica Inhibits TGF- β 1-induced Collagen Expression in Human Keloid Fibroblasts via PPAR- γ Activation. Int J Biol Sci. 2013;9:1032–42.

Ruan JS, Zhou H, Yang L, Wang L, Jiang ZS, Sun H, et al. Ursolic acid attenuates TGF-b1-induced epithelial-mesenchymal transition in NSCLC by targeting integrin Avb5/MMPs signaling. Oncol Res. 2019;27(5):593–600.

DOI: https://doi.org/10.24198/ijpst.v11i2.50623