Immunomodulatory Activity of Purple Button Herb (Borreria laevis Lamk.) on Macrophage Phagocytosis

Mulyadi Prasetyo; Wa Ode Yuliastri; Jasria Pusmarani; Selpirahmawati Saranani; Putri Handayani

doi:10.24198/ijpst.v6i3.53924

Immunomodulatory Activity of Purple Button Herb (Borreria laevis Lamk.) on Macrophage Phagocytosis

Mulyadi Prasetyo, Wa Ode Yuliastri, Jasria Pusmarani, Selpirahmawati Saranani, Putri Handayani

Abstrak

Immunomodulators operate through various mechanisms, one of which involves enhancing phagocytic activity. The purple button herb (Borreria laevis Lamk.) is recognized for containing secondary metabolites that may function as immunomodulators; however, its influence on phagocytosis has not been previously assessed. Consequently, a study explored the immunomodulatory effects of distinct fractions of the purple button herb on macrophage phagocytosis in male mice. Over seven days, the mice were orally administered ethanolic extract, ethyl acetate, and n-hexane fractions of the purple button herb. On the eighth day, the subjects were infected with Staphylococcus aureus. After euthanization, intraperitoneal fluid was collected to analyze macrophage phagocytosis activity. The study's results suggested that the ethanolic extract, ethyl acetate, and n-hexane fractions of the purple button herb tended to enhance macrophage activity, with the n-hexane fraction achieving the highest phagocytosis rate of 84.7%. The corresponding p-value > 0.05 in the LSD test indicated no statistically significant difference when compared to the positive control. In conclusion, the n-hexane fraction of the purple button herb demonstrates potential as an immunostimulant by augmenting macrophage activity.

Kata Kunci

Immunomodulator; Macrophage Phagocytosis; Purple Button Herb

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Referensi

Purkon DB, Nuraisyah ZAN, Namina DR, Roseno MH, Haerussana AENM, Fadhlillah FM, et al. Immunomodulatory activity of Ananas comosus (L.) Merr. flesh and stem fruit juice on mice with carbon clearance and Staphylococcus aures-induced methods. Journal of Research in Pharmacy. 2023;27(6): 2497-510.

Rengganis I and Baratawidjaja KG. Imunologi dasar. Badan Penerbit Fakultas Kedokteran Universitas Indonesia; 2018.

Amalia L and Hiola F. Analisis gejala klinis dan peningkatan kekebalan tubuh untuk mencegah penyakit covid-19. Jambura Journal of Health Sciences and Research. 2020; 2(2): 71-76.

Li N, Li S, Wang Y, Wang Q, Zhou J, Liu J, et.al. Compositional analysis and immunomodulatory activity of blue pigment fraction (BPF) from Laba garlic. Food Chemistry. 2023;406, 134976.

Ulvia R. Pengaruh Ekstrak Air Dan Fraksi Herba Sambiloto [Andrographis paniculata (Burm. F) Nees] Terhadap Respon Imun Melalui Produksi Nitrit Oksida Dan Sitokin Il-1beta Pada Sel RAW 264.7 (Disertasi). Yogyakarta: Universitas Gadjah Mada; 2022.

Istini dan Santoso B. Pengaruh Penambahan Serum dan lama Waktu Inkubasi Lateks terhadap Aktivitas Fagositosis Makrofag Tikus Sprague Dawley (SD) dalam Menunjang Kegiatan Penelitian. Indonesian Journal of Laboratory. 2018;1(1): 16-22.

Kirana IDAA, Bodhi W, Lebang JS, and Fatimawali F. Uji Aktivitas Fagositosis Makrofag Dari Ekstrak Kulit Buah Jeruk Nipis (Citrus aurantifolia) Sebagai Imunomodulator. Jurnal Kesehatan Tambusai. 2023; 4(3): 3021-3027.

Martinus AT, Dachlan AS, and Halim E. Penggunaan Imunostimulan Dalam Bidang Dermatovenerologi. Media Dermato Venereologica Indonesiana. 2019;46(2): 111–5.

Zahrah Z and Cahyani YD. Pelayanan Kefarmasian Pada Pasien Imunosupresif Sebagai Tindakan Preventif di Masa Pandemi COVID-19. Farmaka. 2020;18(2) : 122-31.

Rilansia NPA. Efektivitas dan Keamanan Terapi Imunosupresan pada Pasien Penyakit Autoimun Systemic Lupus Erythematosus dengan Manifestasi Klinis pada Mukokutaneus, Muskoleskeletal, Ginjal, Dan Neuropsychiatric (Disertasi). Bali: Universitas Mahasaraswati Denpasar; 2023.

Shahbazi S and Bolhassani A. Immunostimulants: types and functions. Journal of Medical Microbiology and Infectious Diseases. 2016;4(3): 45–51.

Asep M, Meilawati L, and Hanafi M. Karakterisasi Ekstrak Etanol Herba Katumpangan Air (Peperomia pellucida L. Kunth). Jurnal Biopropal Industri. 2014;6(2): 53–61.

Sidarima JIS. Skrining Fitokimia Dan Penentuan Kadar Polifenol Total, Flavonoid Total, Saporan Total, Dan Tannin Total Herba Kancing Ungu (Borreria laevis Lamk.). (Skripsi). Kendari: Universitas Mandala Waluya; 2020.

Uthia R, Arifin H, and Efrianti F. Pengaruh hasil fraksinasi ekstrak daun kemangi (Ocimum sanctum L.) terhadap aktivitas susunan saraf pusat pada mencit putih jantan. Farmasi Higea. 2017; 9(1): 85–95.

Kasminah. Antivitas Antioksidan Rumpur Laut (Halymenia durvillaei) dengan Pelarut Non Polar, Semi Polar dan Polar. (Skripsi). Surabaya: Universitas Airlangga; 2016.

Nugroho AE, Andrie M, Warditiani NK, Siswanto E, Pramono S, and Lukitaningsih E. Antidiabetic and antihiperlipidemic effect of Andrographis paniculata (Burm. f.) Nees and andrographolide in high-fructose-fat-fed rats. Indian journal of pharmacology. 2012; 44(3): 377-81.

Rauf A, Haeria H, Anas D D. Imunostimulan Fraksi Daun Katuk (Sauropus androgynus L. Merr.) terhadap Aktivitas dan Kapasitas Fagositosis Makrofag pada Mencit Jantan (Mus musculus). Jurnal farmasi UIN Alauddin Makassar. 2017;4(1): 9-15.

Handayani N. Uji aktivitas fagositosis makrofag ekstrak etanol daun suji (Dracaena angustifolia (medik.) Roxb.) Secara in vitro. Jurnal Farmasi Medica/Pharmacy Medical Journal (PMJ). 2018;1(1).

Tong SY, Davis JS, Eichenberger E, Holland TL, and Fowler Jr VG. Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clinical microbiology reviews. 2015;28(3): 603-61.

Weiss G and Schaible UE. Macrophage defense mechanisms against intracellular bacteria. Immunological Reviews. 2015; 264(1): 182–203.

Yuliastri, W. O., Mahmudah, R. A., Fauziah, R., Ridwan, B. A., & Salsyafirah, W. (2023). Formulasi Sediaan Lip Balm Kombinasi Ekstrak Etanol 96% Herba Kancing Ungu (Borreria laevis Lamk.) dan Ekstrak Etanol 96% Kulit Buah Manggis (Garcinia mangostana Linn.) Sebagai Antioksidan. Jurnal Mandala Pharmacon Indonesia, 9(2), 352-363.

Hosseinzade A, Sadeghi O, Naghdipour Biregani A, Soukhtehzari S, Brandt GS, and Esmaillzadeh A. Immunomodulatory effects of flavonoids: possible induction of T CD4+ regulatory cells through suppression of mTOR pathway signaling activity. Frontiers Immunology. 2019; 10:51.

Mendes LF, Gaspar VM, Conde TA, Mano JF, and Duarte IF. Flavonoid-mediated immunomodulation of human macrophages involves key metabolites and metabolic pathways. Scientific Reports. 2019; 9(1):14906.

DOI: https://doi.org/10.24198/ijpst.v6i3.53924